Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102059
Name   oriT_FWSEC0527|unnamed2 in_silico
Organism   Escherichia coli strain FWSEC0527
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_RRRW01000071 (4661..4895 [+], 235 nt)
oriT length   235 nt
IRs (inverted repeats)      192..198, 206..212  (TATAAAA..TTTTATA)
 104..111, 117..124  (TTTAAATA..TATTTAAA)
 40..47, 50..57  (GCAAAAAC..GTTTTTGC)
 4..11, 16..23  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 235 nt

>oriT_FWSEC0527|unnamed2
AGGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTATCTTTAATATCATTGAGTTGTATTTGTGGATTTATATTGTTTAAATATGATTTATTTAAAGCAGCGTCGTTAACGCAGCTACAGCAACGCGCCGACACCGCTTTGTAGGGGTGGTACTGACTATTTTTATAAAAAACATTATTTTATATTAGGGGTGCTGCTAGCGGTGCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1644 GenBank   WP_136756055
Name   traI_C9296_RS26080_FWSEC0527|unnamed2 insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191936.83 Da        Isoelectric Point: 6.0538

>WP_136756055.1 conjugative transfer relaxase/helicase TraI [Escherichia coli]
MMSIAQVRSAGSAGNYYTDKDNYYVLSSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGRNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHQEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSAKVFVSVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLLQRSGETPNFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKADREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGGSPARFIAPGRKYPQPYVALPAFDSNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGTITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

TrwC relaxase (TrwC)

(10-283)

DNA helicase TraI (TraI_C)

(1434-1557)

AAA domain (AAA_30)

(969-1156)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-627)


  Protein structure



No available structure.




Auxiliary protein

ID   657 GenBank   WP_001151530
Name   WP_001151530_FWSEC0527|unnamed2 insolico UniProt ID   A0A385JKG6
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14525.54 Da        Isoelectric Point: 4.8662

>WP_001151530.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacteriaceae]
MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLELGLRVYEAQMERKESAFNQAEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSVEMERFFPKNDDE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-126)


  Protein structure


Source ID Structure
AlphaFold DB A0A385JKG6


T4CP

ID   1273 GenBank   WP_001064245
Name   traC_C9296_RS25975_FWSEC0527|unnamed2 insolico UniProt ID   _
Length   875 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 875 a.a.        Molecular weight: 99159.81 Da        Isoelectric Point: 5.8486

>WP_001064245.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacteriaceae]
MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAA
TQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNVADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIVLLDQYT
ANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR
LLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(289-446)

TraC protein (TraC_F_IV)

(38-276)

TraG P-loop domain (P-loop_TraG)

(467-771)

  Protein structure



No available structure.



ID   1274 GenBank   WP_001603683
Name   traD_C9296_RS26065_FWSEC0527|unnamed2 insolico UniProt ID   _
Length   717 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 717 a.a.        Molecular weight: 81448.92 Da        Isoelectric Point: 5.0504

>WP_001603683.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCTTLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAASLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQTRPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPVSPAI
NDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDIQQQMQRREEVN
INVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 4090..30228

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9296_RS25865 (C9296_25895) 55..489 + 435 WP_000845953 conjugation system SOS inhibitor PsiB -
C9296_RS25870 (C9296_25900) 486..1248 + 763 Protein_1 plasmid SOS inhibition protein A -
C9296_RS27010 1217..1405 - 189 WP_001299721 hypothetical protein -
C9296_RS27670 1427..1576 + 150 Protein_3 plasmid maintenance protein Mok -
C9296_RS25875 (C9296_25905) 1518..1643 + 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
C9296_RS27675 (C9296_25910) 1863..2093 + 231 WP_001426396 hypothetical protein -
C9296_RS27680 2091..2264 - 174 Protein_6 hypothetical protein -
C9296_RS27120 (C9296_25920) 2334..2540 + 207 WP_089649215 single-stranded DNA-binding protein -
C9296_RS25895 (C9296_25925) 2565..2852 + 288 WP_000107542 hypothetical protein -
C9296_RS25900 (C9296_25930) 2973..3794 + 822 Protein_9 DUF932 domain-containing protein -
C9296_RS25905 (C9296_25935) 4090..4737 - 648 WP_123004336 transglycosylase SLT domain-containing protein virB1
C9296_RS25910 (C9296_25940) 4967..5350 + 384 WP_001151530 conjugal transfer relaxosome DNA-binding protein TraM -
C9296_RS25915 (C9296_25945) 5542..6189 + 648 WP_000332528 transcriptional regulator TraJ family protein -
C9296_RS25920 (C9296_25950) 6309..6536 + 228 WP_000589558 conjugal transfer relaxosome protein TraY -
C9296_RS25925 (C9296_25955) 6570..6962 + 393 WP_024127870 type IV conjugative transfer system pilin TraA -
C9296_RS25930 (C9296_25960) 6977..7288 + 312 WP_000012106 type IV conjugative transfer system protein TraL traL
C9296_RS25935 (C9296_25965) 7310..7876 + 567 WP_000399792 type IV conjugative transfer system protein TraE traE
C9296_RS25940 (C9296_25970) 7863..8591 + 729 WP_061336220 type-F conjugative transfer system secretin TraK traK
C9296_RS25945 (C9296_25975) 8591..10018 + 1428 WP_061336221 F-type conjugal transfer pilus assembly protein TraB traB
C9296_RS25950 (C9296_25980) 10008..10598 + 591 WP_061336222 conjugal transfer pilus-stabilizing protein TraP -
C9296_RS25955 (C9296_25985) 10585..10782 + 198 WP_001324648 conjugal transfer protein TrbD -
C9296_RS25960 (C9296_25990) 10794..11045 + 252 WP_001038330 conjugal transfer protein TrbG -
C9296_RS25965 (C9296_25995) 11042..11557 + 516 WP_000809838 type IV conjugative transfer system lipoprotein TraV traV
C9296_RS25970 (C9296_26000) 11692..11913 + 222 WP_001278689 conjugal transfer protein TraR -
C9296_RS25975 (C9296_26005) 12073..14700 + 2628 WP_001064245 type IV secretion system protein TraC virb4
C9296_RS25980 (C9296_26010) 14697..15083 + 387 WP_000099690 type-F conjugative transfer system protein TrbI -
C9296_RS25985 (C9296_26015) 15080..15712 + 633 WP_001203717 type-F conjugative transfer system protein TraW traW
C9296_RS25990 (C9296_26020) 15709..16701 + 993 WP_000830183 conjugal transfer pilus assembly protein TraU traU
C9296_RS25995 (C9296_26025) 16710..17348 + 639 WP_000777695 type-F conjugative transfer system pilin assembly protein TrbC trbC
C9296_RS26000 (C9296_26030) 17345..19153 + 1809 WP_061336223 type-F conjugative transfer system mating-pair stabilization protein TraN traN
C9296_RS26005 (C9296_26035) 19180..19440 + 261 WP_000864348 conjugal transfer protein TrbE -
C9296_RS26010 (C9296_26040) 19433..20176 + 744 WP_001030396 type-F conjugative transfer system pilin assembly protein TraF traF
C9296_RS26015 (C9296_26045) 20192..20539 + 348 WP_061336225 conjugal transfer protein TrbA -
C9296_RS26020 (C9296_26050) 20541..20816 - 276 WP_001513501 hypothetical protein -
C9296_RS26025 (C9296_26055) 20897..21181 + 285 WP_000624108 type-F conjugative transfer system pilin chaperone TraQ -
C9296_RS26030 (C9296_26060) 21168..21713 + 546 WP_000059833 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
C9296_RS26035 (C9296_26065) 21643..21984 + 342 WP_001442101 P-type conjugative transfer protein TrbJ -
C9296_RS26040 (C9296_26070) 21971..22363 + 393 WP_032082924 F-type conjugal transfer protein TrbF -
C9296_RS26045 (C9296_26075) 22350..23723 + 1374 WP_136756054 conjugal transfer pilus assembly protein TraH traH
C9296_RS26050 (C9296_26080) 23720..26542 + 2823 WP_061336227 conjugal transfer mating-pair stabilization protein TraG traG
C9296_RS26055 (C9296_26085) 26558..27043 + 486 WP_000605868 hypothetical protein -
C9296_RS26060 (C9296_26090) 27092..27823 + 732 WP_000782451 conjugal transfer complement resistance protein TraT -
C9296_RS26065 (C9296_26095) 28075..30228 + 2154 WP_001603683 type IV conjugative transfer system coupling protein TraD virb4
C9296_RS26070 (C9296_26100) 30237..30635 - 399 WP_000911317 type II toxin-antitoxin system VapC family toxin -
C9296_RS26075 (C9296_26105) 30635..30862 - 228 WP_000450532 toxin-antitoxin system antitoxin VapB -

Host bacterium

ID   2503 GenBank   NZ_RRRW01000071
Plasmid name   FWSEC0527|unnamed2 Incompatibility group   -
Plasmid size   93340 bp Coordinate of oriT [Strand]   4661..4895 [+]
Host baterium   Escherichia coli strain FWSEC0527

Cargo genes

Drug resistance gene   -
Virulence gene   hlyC, hlyA, hlyB, hlyD, cnf1, cdtA, cdtB, cdtC
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -