Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102056
Name   oriT_kp10|unnamed1 KP10_27 in_silico
Organism   Klebsiella pneumoniae strain kp10
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MNCK01000027 (21623..21704 [+], 82 nt)
oriT length   82 nt
IRs (inverted repeats)      27..32, 35..40  (GCCACC..GGTGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_kp10|unnamed1 KP10_27
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1643 GenBank   WP_032440569
Name   Relaxase_BLA25_RS25400_kp10|unnamed1 KP10_27 insolico UniProt ID   A0A7G3L4G1
Length   642 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 642 a.a.        Molecular weight: 74384.24 Da        Isoelectric Point: 8.3203

>WP_032440569.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-307)


  Protein structure


Source ID Structure
AlphaFold DB A0A7G3L4G1


T4CP

ID   1272 GenBank   WP_032440593
Name   trbC_BLA25_RS25535_kp10|unnamed1 KP10_27 insolico UniProt ID   _
Length   730 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 730 a.a.        Molecular weight: 82679.78 Da        Isoelectric Point: 6.3201

>WP_032440593.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 30813..52854

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BLA25_RS25410 (BLA25_25420) 25960..26454 - 495 WP_032440571 molybdopterin-dependent oxidoreductase -
BLA25_RS25415 (BLA25_25425) 26447..27376 - 930 WP_032440581 hypothetical protein -
BLA25_RS25420 (BLA25_25430) 27436..28278 - 843 WP_032440572 helix-turn-helix domain-containing protein -
BLA25_RS25425 (BLA25_25435) 28888..29115 + 228 WP_032440573 hypothetical protein -
BLA25_RS25430 (BLA25_25440) 29847..30326 + 480 WP_032440574 lytic transglycosylase domain-containing protein -
BLA25_RS25435 (BLA25_25445) 30352..30816 + 465 WP_225546581 DotD/TraH family lipoprotein -
BLA25_RS25440 (BLA25_25450) 30813..31631 + 819 WP_032440576 type IV secretory system conjugative DNA transfer family protein traI
BLA25_RS25445 (BLA25_25455) 31609..32796 + 1188 WP_077255878 plasmid transfer ATPase TraJ -
BLA25_RS25450 (BLA25_25460) 32793..33059 + 267 WP_032440577 IcmT/TraK family protein traK
BLA25_RS25455 (BLA25_25465) 33077..36598 + 3522 WP_071182200 LPD7 domain-containing protein -
BLA25_RS25460 (BLA25_25470) 36632..36985 + 354 WP_032440584 hypothetical protein traL
BLA25_RS25465 (BLA25_25475) 37057..37725 + 669 WP_032440585 DotI/IcmL/TraM family protein traM
BLA25_RS30235 37736..38839 + 1104 WP_077264204 DotH/IcmK family type IV secretion protein traN
BLA25_RS25475 (BLA25_25485) 38843..40183 + 1341 WP_032440586 conjugal transfer protein TraO traO
BLA25_RS25480 (BLA25_25490) 40190..40909 + 720 WP_050597333 conjugal transfer protein TraP traP
BLA25_RS25485 (BLA25_25495) 40919..41452 + 534 WP_032440587 conjugal transfer protein TraQ traQ
BLA25_RS25490 (BLA25_25500) 41508..41909 + 402 WP_032440588 DUF6750 family protein traR
BLA25_RS31120 41953..42516 + 564 WP_045325070 hypothetical protein traT
BLA25_RS25500 (BLA25_25510) 42526..45573 + 3048 WP_032440589 conjugal transfer protein traU
BLA25_RS25505 (BLA25_25515) 45583..46779 + 1197 WP_032440590 conjugal transfer protein TraW traW
BLA25_RS25510 (BLA25_25520) 46776..47357 + 582 WP_050597335 conjugal transfer protein TraX -
BLA25_RS25515 (BLA25_25525) 47399..49543 + 2145 WP_032440591 DotA/TraY family protein traY
BLA25_RS25520 (BLA25_25530) 49609..50232 + 624 WP_032440592 plasmid IncI1-type surface exclusion protein ExcA -
BLA25_RS25525 (BLA25_25535) 50334..51752 + 1419 WP_063666705 hypothetical protein trbA
BLA25_RS25530 (BLA25_25540) 51763..52854 + 1092 WP_050597336 thioredoxin fold domain-containing protein trbB
BLA25_RS25535 (BLA25_25545) 52856..55048 + 2193 WP_032440593 F-type conjugative transfer protein TrbC -
BLA25_RS25540 (BLA25_25550) 55094..55627 + 534 WP_032440594 phospholipase D family protein -
BLA25_RS31125 55639..55890 + 252 WP_113394424 hypothetical protein -
BLA25_RS25545 (BLA25_25555) 56396..57091 + 696 WP_254910806 RepA family replication protein -

Host bacterium

ID   2500 GenBank   NZ_MNCK01000027
Plasmid name   kp10|unnamed1 KP10_27 Incompatibility group   IncFII
Plasmid size   61712 bp Coordinate of oriT [Strand]   21623..21704 [+]
Host baterium   Klebsiella pneumoniae strain kp10

Cargo genes

Drug resistance gene   qnrS1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -