Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102047
Name   oriT_FWSEC0527|unnamed1 in_silico
Organism   Escherichia coli strain FWSEC0527
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_RRRW01000067 (4926..5014 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_FWSEC0527|unnamed1
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1636 GenBank   WP_001354015
Name   nikB_C9296_RS25410_FWSEC0527|unnamed1 insolico UniProt ID   E8XLT1
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104018.47 Da        Isoelectric Point: 7.3526

>WP_001354015.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCLAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPENRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB E8XLT1


Auxiliary protein

ID   656 GenBank   WP_001283946
Name   WP_001283946_FWSEC0527|unnamed1 insolico UniProt ID   _
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12599.54 Da        Isoelectric Point: 10.7463

>WP_001283946.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSDVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure



No available structure.




T4CP

ID   1271 GenBank   WP_136756048
Name   trbC_C9296_RS25415_FWSEC0527|unnamed1 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86922.06 Da        Isoelectric Point: 6.6907

>WP_136756048.1 F-type conjugative transfer protein TrbC [Escherichia coli]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIHDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 10392..46605

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9296_RS25415 (C9296_25445) 8108..10399 - 2292 WP_136756048 F-type conjugative transfer protein TrbC -
C9296_RS25420 (C9296_25450) 10392..11462 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
C9296_RS25425 (C9296_25455) 11481..12689 - 1209 WP_061336178 IncI1-type conjugal transfer protein TrbA trbA
C9296_RS25430 (C9296_25460) 12981..13133 + 153 WP_024179514 Hok/Gef family protein -
C9296_RS25435 (C9296_25465) 13205..13456 - 252 WP_001291965 hypothetical protein -
C9296_RS27840 14580..14678 + 99 WP_022630883 DinQ-like type I toxin DqlB -
C9296_RS26995 14740..14916 - 177 WP_001054898 hypothetical protein -
C9296_RS25445 (C9296_25475) 15125..15334 - 210 WP_015059315 hemolysin expression modulator Hha -
C9296_RS25450 (C9296_25480) 15432..16046 - 615 WP_015059316 plasmid IncI1-type surface exclusion protein ExcA -
C9296_RS25455 (C9296_25485) 16122..18290 - 2169 WP_000698355 IncI1-type conjugal transfer membrane protein TraY traY
C9296_RS25460 (C9296_25490) 18387..18971 - 585 WP_061336176 IncI1-type conjugal transfer protein TraX -
C9296_RS25465 (C9296_25495) 19000..20202 - 1203 WP_061336175 IncI1-type conjugal transfer protein TraW traW
C9296_RS25470 (C9296_25500) 20169..20783 - 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
C9296_RS25475 (C9296_25505) 20783..23827 - 3045 WP_136756049 IncI1-type conjugal transfer protein TraU traU
C9296_RS25480 (C9296_25510) 23917..24717 - 801 WP_001493813 IncI1-type conjugal transfer protein TraT traT
C9296_RS25485 (C9296_25515) 24701..24889 - 189 WP_001277253 putative conjugal transfer protein TraS -
C9296_RS25490 (C9296_25520) 24953..25357 - 405 WP_061336173 IncI1-type conjugal transfer protein TraR traR
C9296_RS25495 (C9296_25525) 25409..25936 - 528 WP_001055569 conjugal transfer protein TraQ traQ
C9296_RS25500 (C9296_25530) 25936..26640 - 705 WP_061336172 IncI1-type conjugal transfer protein TraP traP
C9296_RS25505 (C9296_25535) 26640..27929 - 1290 WP_061336171 conjugal transfer protein TraO traO
C9296_RS25510 (C9296_25540) 27932..28915 - 984 WP_001191877 IncI1-type conjugal transfer protein TraN traN
C9296_RS25515 (C9296_25545) 28926..29618 - 693 WP_000138551 DotI/IcmL family type IV secretion protein traM
C9296_RS25520 (C9296_25550) 29615..29962 - 348 WP_001055900 conjugal transfer protein traL
C9296_RS25525 (C9296_25555) 29980..33747 - 3768 WP_061336170 LPD7 domain-containing protein -
C9296_RS25530 (C9296_25560) 33837..34388 - 552 WP_047666746 phospholipase D family protein -
C9296_RS25535 (C9296_25565) 34403..34693 - 291 WP_001314267 hypothetical protein traK
C9296_RS25540 (C9296_25570) 34690..35838 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
C9296_RS25545 (C9296_25575) 35835..36653 - 819 WP_021555364 IncI1-type conjugal transfer lipoprotein TraI traI
C9296_RS25550 (C9296_25580) 36650..37108 - 459 WP_061336168 IncI1-type conjugal transfer lipoprotein TraH -
C9296_RS25555 (C9296_25585) 37503..38087 - 585 WP_000977523 histidine phosphatase family protein -
C9296_RS25560 (C9296_25590) 38147..39349 - 1203 WP_061336167 conjugal transfer protein TraF -
C9296_RS25565 (C9296_25595) 39435..40259 - 825 WP_001238935 conjugal transfer protein TraE traE
C9296_RS27975 40410..40502 - 93 Protein_42 shufflon-specific DNA recombinase -
C9296_RS25575 (C9296_25605) 40506..40763 + 258 Protein_43 transposase -
C9296_RS25580 (C9296_25610) 40820..42112 - 1293 WP_001619585 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
C9296_RS25585 (C9296_25615) 42112..42768 - 657 WP_001401940 A24 family peptidase -
C9296_RS25590 (C9296_25620) 42753..43313 - 561 WP_061336166 lytic transglycosylase domain-containing protein virB1
C9296_RS25595 (C9296_25625) 43323..43937 - 615 WP_061336165 type 4 pilus major pilin -
C9296_RS25600 (C9296_25630) 43954..45039 - 1086 WP_061336164 type II secretion system F family protein -
C9296_RS25605 (C9296_25635) 45052..46605 - 1554 WP_061336163 ATPase, T2SS/T4P/T4SS family virB11
C9296_RS25610 (C9296_25640) 46616..47068 - 453 WP_001247333 type IV pilus biogenesis protein PilP -
C9296_RS25615 (C9296_25645) 47055..48350 - 1296 WP_061336162 type 4b pilus protein PilO2 -
C9296_RS25620 (C9296_25650) 48343..50025 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
C9296_RS25625 (C9296_25655) 50039..50476 - 438 WP_061336161 type IV pilus biogenesis protein PilM -
C9296_RS25630 (C9296_25660) 50476..51543 - 1068 WP_061336160 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   2491 GenBank   NZ_RRRW01000067
Plasmid name   FWSEC0527|unnamed1 Incompatibility group   IncI
Plasmid size   58444 bp Coordinate of oriT [Strand]   4926..5014 [-]
Host baterium   Escherichia coli strain FWSEC0527

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -