Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   102038
Name   oriT_FWSEC0595|unnamed2 in_silico
Organism   Escherichia coli strain FWSEC0595
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_RRSY01000090 (44233..44356 [-], 124 nt)
oriT length   124 nt
IRs (inverted repeats)      92..99, 113..120  (ATAATGTA..TACATTAT)
 90..95, 107..112  (AAATAA..TTATTT)
 71..76, 83..88  (AATCAT..ATGATT)
 39..46, 49..56  (GCAAAAAC..GTTTTTGC)
 3..10, 15..22  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 124 nt

>oriT_FWSEC0595|unnamed2
GGTTGGTGGTTCTCACCACCAAAAGCACCACACACTACGCAAAAACAAGTTTTTGCTGATTTGATATTTGAATCATTAACTTATGATTTAAATAATGTATTTTAATTTATTTTACATTATAAAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1629 GenBank   WP_136750748
Name   traI_C9326_RS24340_FWSEC0595|unnamed2 insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191459.04 Da        Isoelectric Point: 5.9032

>WP_136750748.1 conjugative transfer relaxase/helicase TraI [Escherichia coli]
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQGSVDKDVFTRLLEGKLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIRAAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDVADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
AGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAVSVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTVLSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKIPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAPLPVSDSPFTALKL
ESGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISHQKSALHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELRETVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSLSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISRDDGLITLEDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPAQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAISKAVQKGTAHDVL
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAQEKPDQP
DGKTEQAVRAIAGQEQDRASSSERETALPESVLRESQRVQDAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

DNA helicase TraI (TraI_C)

(1434-1557)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)

AAA domain (AAA_30)

(968-1155)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)

TrwC relaxase (TrwC)

(10-283)


  Protein structure



No available structure.




Auxiliary protein

ID   654 GenBank   WP_032142519
Name   WP_032142519_FWSEC0595|unnamed2 insolico UniProt ID   _
Length   75 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 75 a.a.        Molecular weight: 9106.17 Da        Isoelectric Point: 10.3319

>WP_032142519.1 conjugal transfer relaxosome protein TraY [Escherichia coli]
MRRRNARGGTSRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIRLRDHLKRYPDFYNEEIFREVTEERES
TFKEL

  Protein domains


Predicted by InterproScan.

TraY domain (TraY)

(14-61)


  Protein structure



No available structure.



ID   655 GenBank   WP_021521311
Name   WP_021521311_FWSEC0595|unnamed2 insolico UniProt ID   _
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14520.47 Da        Isoelectric Point: 4.7279

>WP_021521311.1 conjugal transfer relaxosome DNA-binding protein TraM [Escherichia coli]
MAKVQAYVSDEIVYKINEIVERRRAEGAKSTDVSFSSISTMLLELGLRVYEAQMERKESSFNQTEFNKVL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPKNDEE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-126)


  Protein structure



No available structure.




T4CP

ID   1269 GenBank   WP_123129820
Name   traD_C9326_RS24345_FWSEC0595|unnamed2 insolico UniProt ID   _
Length   735 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 735 a.a.        Molecular weight: 83772.54 Da        Isoelectric Point: 5.1071

>WP_123129820.1 type IV conjugative transfer system coupling protein TraD [Escherichia coli]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCTTLEGMR
DLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQAEQPQQPQQPQQPQQ
PQQPQQPQQPQQPVFPVINDKKSDTGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVMDMAVYEAWQ
REQNPDIQQQMQRREEVNINVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-560)

  Protein structure



No available structure.



ID   1270 GenBank   WP_000069759
Name   traC_C9326_RS24450_FWSEC0595|unnamed2 insolico UniProt ID   _
Length   876 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 876 a.a.        Molecular weight: 99406.07 Da        Isoelectric Point: 5.6251

>WP_000069759.1 MULTISPECIES: type IV secretion system protein TraC [Escherichia]
MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAI
PINGANESIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAA
ETLFPLPEGLNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGAYITTQTVDAQAFIDIV
GEMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAF
LWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWG
ELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGK
GLFRQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSG
AGKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERIRDQLS
VMASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIVLLDQY
TANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGW
RLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKY
NQLFPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRQEG
LSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(468-771)

Family of unknown function (DUF5934) (DUF5934)

(290-447)

TraC protein (TraC_F_IV)

(39-277)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 15296..44928

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9326_RS24345 (C9326_24370) 15296..17503 - 2208 WP_123129820 type IV conjugative transfer system coupling protein TraD virb4
C9326_RS24350 (C9326_24375) 17785..18264 - 480 WP_001473214 hypothetical protein -
C9326_RS24355 (C9326_24380) 18468..19199 - 732 WP_000850428 conjugal transfer complement resistance protein TraT -
C9326_RS24360 (C9326_24385) 19213..19722 - 510 WP_047081613 conjugal transfer entry exclusion protein TraS -
C9326_RS24365 (C9326_24390) 19719..22544 - 2826 WP_047081612 conjugal transfer mating-pair stabilization protein TraG traG
C9326_RS24370 (C9326_24395) 22541..23914 - 1374 WP_000944332 conjugal transfer pilus assembly protein TraH traH
C9326_RS24375 (C9326_24400) 23901..24293 - 393 WP_024213667 F-type conjugal transfer protein TrbF -
C9326_RS24380 (C9326_24405) 24274..24621 - 348 WP_071531747 P-type conjugative transfer protein TrbJ -
C9326_RS24385 (C9326_24410) 24551..25096 - 546 WP_001477377 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
C9326_RS24390 (C9326_24415) 25083..25370 - 288 WP_000335419 type-F conjugative transfer system pilin chaperone TraQ -
C9326_RS24395 (C9326_24420) 25436..25837 - 402 WP_047081617 hypothetical protein -
C9326_RS25430 (C9326_24425) 25968..26332 - 365 Protein_29 hypothetical protein -
C9326_RS24405 (C9326_24430) 26346..27089 - 744 WP_001030385 type-F conjugative transfer system pilin assembly protein TraF traF
C9326_RS24410 (C9326_24435) 27082..27339 - 258 WP_000864355 conjugal transfer protein TrbE -
C9326_RS24415 (C9326_24440) 27353..29257 - 1905 WP_047081608 type-F conjugative transfer system mating-pair stabilization protein TraN traN
C9326_RS24420 (C9326_24445) 29351..29731 - 381 WP_000056343 hypothetical protein -
C9326_RS24425 (C9326_24450) 29728..30366 - 639 WP_047081603 type-F conjugative transfer system pilin assembly protein TrbC trbC
C9326_RS24430 (C9326_24455) 30472..30651 - 180 WP_000970212 hypothetical protein -
C9326_RS24435 (C9326_24460) 30672..31664 - 993 WP_000817328 conjugal transfer pilus assembly protein TraU traU
C9326_RS24440 (C9326_24465) 31661..32293 - 633 WP_047081601 type-F conjugative transfer system protein TraW traW
C9326_RS24445 (C9326_24470) 32290..32676 - 387 WP_047081599 type-F conjugative transfer system protein TrbI -
C9326_RS24450 (C9326_24475) 32673..35303 - 2631 WP_000069759 type IV secretion system protein TraC virb4
C9326_RS24455 (C9326_24480) 35428..35775 - 348 WP_000836684 hypothetical protein -
C9326_RS25225 (C9326_24485) 35803..36021 - 219 WP_071528664 hypothetical protein -
C9326_RS24465 (C9326_24490) 36101..36574 - 474 WP_000549599 hypothetical protein -
C9326_RS24470 (C9326_24495) 36585..36980 - 396 WP_000549593 hypothetical protein -
C9326_RS24475 (C9326_24500) 36973..37194 - 222 WP_001278977 conjugal transfer protein TraR -
C9326_RS24480 (C9326_24505) 37329..37844 - 516 WP_000809868 type IV conjugative transfer system lipoprotein TraV traV
C9326_RS24485 (C9326_24510) 37841..38092 - 252 WP_001038341 conjugal transfer protein TrbG -
C9326_RS24490 (C9326_24515) 38085..38405 - 321 WP_001057270 conjugal transfer protein TrbD virb4
C9326_RS24495 (C9326_24520) 38392..38979 - 588 WP_047081597 conjugal transfer pilus-stabilizing protein TraP -
C9326_RS24500 (C9326_24525) 38969..40399 - 1431 WP_000146622 F-type conjugal transfer pilus assembly protein TraB traB
C9326_RS24505 (C9326_24530) 40399..41127 - 729 WP_021573902 type-F conjugative transfer system secretin TraK traK
C9326_RS24510 (C9326_24535) 41114..41680 - 567 WP_000399759 type IV conjugative transfer system protein TraE traE
C9326_RS24515 (C9326_24540) 41702..42013 - 312 WP_000012099 type IV conjugative transfer system protein TraL traL
C9326_RS24520 (C9326_24545) 42028..42381 - 354 WP_021521309 type IV conjugative transfer system pilin TraA -
C9326_RS24525 (C9326_24550) 42416..42643 - 228 WP_032142519 conjugal transfer relaxosome protein TraY -
C9326_RS24530 (C9326_24555) 42731..43420 - 690 WP_047081595 PAS domain-containing protein -
C9326_RS24535 (C9326_24560) 43613..43996 - 384 WP_021521311 conjugal transfer relaxosome DNA-binding protein TraM -
C9326_RS24540 (C9326_24565) 44338..44928 + 591 WP_000252679 transglycosylase SLT domain-containing protein virB1
C9326_RS24545 (C9326_24570) 45225..46046 - 822 WP_001234463 DUF932 domain-containing protein -
C9326_RS24550 (C9326_24575) 46165..46452 - 288 WP_000107526 hypothetical protein -
C9326_RS24560 (C9326_24585) 46608..46910 - 303 WP_001272235 hypothetical protein -
C9326_RS24830 (C9326_24600) 47227..47506 + 280 Protein_61 hypothetical protein -
C9326_RS24580 (C9326_24605) 47806..47928 - 123 WP_223200913 Hok/Gef family protein -
C9326_RS25230 47873..48025 - 153 Protein_63 DUF5431 family protein -
C9326_RS24585 (C9326_24610) 48175..48489 - 315 WP_000872084 hypothetical protein -
C9326_RS24590 (C9326_24615) 48486..49205 - 720 WP_000116348 plasmid SOS inhibition protein A -
C9326_RS24595 (C9326_24620) 49202..49636 - 435 WP_032209731 conjugation system SOS inhibitor PsiB -

Host bacterium

ID   2482 GenBank   NZ_RRSY01000090
Plasmid name   FWSEC0595|unnamed2 Incompatibility group   IncFII
Plasmid size   52723 bp Coordinate of oriT [Strand]   44233..44356 [-]
Host baterium   Escherichia coli strain FWSEC0595

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -