Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 102026 |
| Name | oriT_ISU 919|unnamed1  |
| Organism | Staphylococcus aureus strain ISU 919 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_LKWS01000078 (2747..2950 [+], 204 nt) |
| oriT length | 204 nt |
| IRs (inverted repeats) | 125..130, 143..148 (AAATCC..GGATTT) 66..72, 83..89 (ACCCCCC..GGGGGGT) |
| Location of nic site | 135..136 |
| Conserved sequence flanking the nic site |
TTTGGTTACA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 204 nt
>oriT_ISU 919|unnamed1
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGAT
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1617 | GenBank | WP_031919544 |
| Name | mobT_APV55_RS07820_ISU 919|unnamed1 |
UniProt ID | _ |
| Length | 401 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 401 a.a. Molecular weight: 47432.12 Da Isoelectric Point: 6.5091
>WP_031919544.1 MobT family relaxase [Staphylococcus aureus]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDFQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDFQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 1267 | GenBank | WP_000813488 |
| Name | tcpA_APV55_RS07810_ISU 919|unnamed1 |
UniProt ID | _ |
| Length | 461 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 461 a.a. Molecular weight: 53370.27 Da Isoelectric Point: 9.0687
>WP_000813488.1 MULTISPECIES: FtsK/SpoIIIE domain-containing protein [Bacteria]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 642..11825
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| APV55_RS07790 (APV55_00610) | 1..273 | + | 273 | WP_142296212 | transposase | - |
| APV55_RS07800 (APV55_00615) | 642..956 | + | 315 | WP_000420682 | YdcP family protein | orf23 |
| APV55_RS07805 (APV55_00620) | 972..1358 | + | 387 | WP_000985015 | YdcP family protein | orf23 |
| APV55_RS07810 (APV55_00625) | 1387..2772 | + | 1386 | WP_000813488 | FtsK/SpoIIIE domain-containing protein | virb4 |
| APV55_RS07815 (APV55_00630) | 2775..2927 | + | 153 | WP_000879507 | hypothetical protein | - |
| APV55_RS07820 (APV55_00635) | 2950..4155 | + | 1206 | WP_031919544 | MobT family relaxase | - |
| APV55_RS07825 (APV55_00640) | 4198..4419 | + | 222 | WP_001009056 | hypothetical protein | orf19 |
| APV55_RS07830 (APV55_00645) | 4536..5033 | + | 498 | WP_000342539 | antirestriction protein ArdA | - |
| APV55_RS07835 (APV55_00650) | 5122..5514 | + | 393 | WP_000723888 | conjugal transfer protein | orf17a |
| APV55_RS07840 (APV55_00655) | 5498..7945 | + | 2448 | WP_071938142 | ATP-binding protein | virb4 |
| APV55_RS07845 (APV55_00660) | 7948..10125 | + | 2178 | WP_000804748 | membrane protein | orf15 |
| APV55_RS07850 (APV55_00665) | 10122..11123 | + | 1002 | WP_000769868 | bifunctional lysozyme/C40 family peptidase | orf14 |
| APV55_RS07855 (APV55_00670) | 11120..11825 | + | 706 | WP_095303798 | conjugal transfer protein | orf13 |
Host bacterium
| ID | 2470 | GenBank | NZ_LKWS01000078 |
| Plasmid name | ISU 919|unnamed1 | Incompatibility group | - |
| Plasmid size | 11825 bp | Coordinate of oriT [Strand] | 2747..2950 [+] |
| Host baterium | Staphylococcus aureus strain ISU 919 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |