Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 102010
• Name: oriT1_ISU 1010|unnamed3
• Organism: Staphylococcus aureus strain ISU 1010
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_LKVP01000004 (1..33 [-], 33 nt)
• oriT length: 33 nt
• IRs (inverted repeats): 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 33 nt

>oriT1_ISU 1010|unnamed3
CACTTTATGAATATAAAGTATAGTGTGTTATAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_APV50_RS01000_ISU 1010|unnamed3

• ID: 1604
• GenBank: WP_015382137
• Name: mobV_APV50_RS01000_ISU 1010|unnamed3
• UniProt ID: L8BAH8
• Length: 409 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 409 a.a.
• Molecular weight: 48447.75 Da
• Isoelectric Point: 7.0106

>WP_015382137.1 MULTISPECIES: MobV family relaxase [Bacilli]
MSMIVARMQKMKAENLVGIGNHNQRKTKNHSNPDIDTSLSKLNYDLVDRTQNYKTDIENFINENKTTTRA
VRKDAVLVNEWIISSDKDFFDNLTESEIENFFERSKDYFAEKFGEKNIRYATVHLDESTPHMHMGIVPFD
KDNKLSAKRVFNRQALRDVQEELPKYLQDFQFEIERGQKGSERKNLTVPEFKKLKEEEREIKKELEIKKD
ELMAYTKENKIDKEIDITPIKEMEDVEIETDEKSLFGITKTKTVRQWTGNIVLSEKDYLKLRQKVNKGKQ
AEGKLEAILETDVYQENKELKNELKDQIDKNDKDIDDYNDLVKRYNNLYEENTSLKNQIGDLKEEIKLIY
QSTKRFFKDRISDFKAFKEVFKELADSISNISREKGLDSSFKKEFDRENKKKRTKGMSR

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: L8BAH8

Host bacterium

• ID: 2454
• GenBank: NZ_LKVP01000004
• Plasmid name: ISU 1010|unnamed3
• Incompatibility group: -
• Plasmid size: 10392 bp
• Coordinate of oriT [Strand]: 1..33 [-]; 9262..9302 [-]
• Host baterium: Staphylococcus aureus strain ISU 1010

Cargo genes

• Drug resistance gene: tet(L), aadD, tet(T)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -