Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 102007
• Name: oriT_ISU 917|unnamed2
• Organism: Staphylococcus aureus strain ISU 917
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_LKWQ01000091 (507..710 [+], 204 nt)
• oriT length: 204 nt
• IRs (inverted repeats): 125..130, 143..148 (AAATCC..GGATTT) 66..72, 83..89 (ACCCCCC..GGGGGGT)
• Location of nic site: 135..136
• Conserved sequence flanking the nic site: TTTGGTTACA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 204 nt

>oriT_ISU 917|unnamed2
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGAT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobT_APV76_RS12395_ISU 917|unnamed2

• ID: 1601
• GenBank: WP_031919544
• Name: mobT_APV76_RS12395_ISU 917|unnamed2
• UniProt ID: _
• Length: 401 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 401 a.a.
• Molecular weight: 47432.12 Da
• Isoelectric Point: 6.5091

>WP_031919544.1 MobT family relaxase [Staphylococcus aureus]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDFQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK

Protein domains

Predicted by InterproScan.

• Replication initiation factor (Rep_trans) (169-373)

• Helix-turn-helix (HTH_3) (15-53)

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (73-161)

Protein structure

No available structure.

Host bacterium

• ID: 2451
• GenBank: NZ_LKWQ01000091
• Plasmid name: ISU 917|unnamed2
• Incompatibility group: -
• Plasmid size: 5147 bp
• Coordinate of oriT [Strand]: 507..710 [+]
• Host baterium: Staphylococcus aureus strain ISU 917

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -