Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 102000
• Name: oriT_ISU 1000|unnamed2
• Organism: Staphylococcus aureus strain ISU 1000
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_LKVI01000174 (1379..1417 [+], 39 nt)
• oriT length: 39 nt
• IRs (inverted repeats): 12..20, 27..35 (TAAAGTATA..TATACTTTA) 1..6, 11..16 (CTTTAT..ATAAAG)
• Location of nic site: 25..26
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 39 nt

>oriT_ISU 1000|unnamed2
CTTTATGAATATAAAGTATAGTGTGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_APV32_RS08680_ISU 1000|unnamed2

• ID: 1597
• GenBank: WP_015382137
• Name: mobV_APV32_RS08680_ISU 1000|unnamed2
• UniProt ID: L8BAH8
• Length: 409 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 409 a.a.
• Molecular weight: 48447.75 Da
• Isoelectric Point: 7.0106

>WP_015382137.1 MULTISPECIES: MobV family relaxase [Bacilli]
MSMIVARMQKMKAENLVGIGNHNQRKTKNHSNPDIDTSLSKLNYDLVDRTQNYKTDIENFINENKTTTRA
VRKDAVLVNEWIISSDKDFFDNLTESEIENFFERSKDYFAEKFGEKNIRYATVHLDESTPHMHMGIVPFD
KDNKLSAKRVFNRQALRDVQEELPKYLQDFQFEIERGQKGSERKNLTVPEFKKLKEEEREIKKELEIKKD
ELMAYTKENKIDKEIDITPIKEMEDVEIETDEKSLFGITKTKTVRQWTGNIVLSEKDYLKLRQKVNKGKQ
AEGKLEAILETDVYQENKELKNELKDQIDKNDKDIDDYNDLVKRYNNLYEENTSLKNQIGDLKEEIKLIY
QSTKRFFKDRISDFKAFKEVFKELADSISNISREKGLDSSFKKEFDRENKKKRTKGMSR

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: L8BAH8

Host bacterium

• ID: 2444
• GenBank: NZ_LKVI01000174
• Plasmid name: ISU 1000|unnamed2
• Incompatibility group: -
• Plasmid size: 9897 bp
• Coordinate of oriT [Strand]: 1379..1417 [+]
• Host baterium: Staphylococcus aureus strain ISU 1000

Cargo genes

• Drug resistance gene: tet(T), aadD
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -