Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 101999
• Name: oriT_ISU 1001|unnamed5
• Organism: Staphylococcus aureus strain ISU 1001
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_LKVJ01000088 (8355..8395 [+], 41 nt)
• oriT length: 41 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGWGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 41 nt

>oriT_ISU 1001|unnamed5
CACTTTATGAATATAAAGTATAGTGWGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_APV37_RS06875_ISU 1001|unnamed5

• ID: 1596
• GenBank: WP_095264438
• Name: mobV_APV37_RS06875_ISU 1001|unnamed5
• UniProt ID: _
• Length: 343 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 343 a.a.
• Molecular weight: 40524.66 Da
• Isoelectric Point: 5.3292

>WP_095264438.1 MobV family relaxase, partial [Staphylococcus aureus]
MSMIVARMQKMKAENLVGIGNHNQRKTKNHSNPDIDTSLSKLNYDLVDRTQNYKTDIENFINENKTTTRA
VRKDAVLVNEWIISSDKDFFDNLTESEIENFFERSKDYFAEKFGEKNIRYATVHLDESTPHMHMGIVPFD
KDNKLSAKRVFNRQALRDVQEELPKYLQDFQFEIERGQKGSERKNLTVPEFKKLKEEEREIKKELEIKKD
ELMAYTKENKIDKEIDITPIKEMEDVEIETDEKSLFGITKTKTVRQWTGNIVLSEKDYLKLRQKVNKGKQ
AEGKLEAILETDVYQENKELKNELKDQIDKNDKDIDDYNDLVKRYNNLYEENTSLKNQIGDLK

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

No available structure.

Host bacterium

• ID: 2443
• GenBank: NZ_LKVJ01000088
• Plasmid name: ISU 1001|unnamed5
• Incompatibility group: -
• Plasmid size: 9464 bp
• Coordinate of oriT [Strand]: 8355..8395 [+]
• Host baterium: Staphylococcus aureus strain ISU 1001

Cargo genes

• Drug resistance gene: tet(T), aadD, tet(L)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -