Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101967 |
| Name | oriT_FWSEC0530|unnamed2  |
| Organism | Escherichia coli strain FWSEC0530 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_RRRY01000068 (42346..42399 [-], 54 nt) |
| oriT length | 54 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 54 nt
>oriT_FWSEC0530|unnamed2
CACAAGCATTGTAACATGCCCGGAACGGGCTTGTGTACAAAGCTTATCGTGCCT
CACAAGCATTGTAACATGCCCGGAACGGGCTTGTGTACAAAGCTTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 1248 | GenBank | WP_000338974 |
| Name | t4cp2_C9299_RS24150_FWSEC0530|unnamed2 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73347.89 Da Isoelectric Point: 9.1391
>WP_000338974.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2328..24777
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C9299_RS25375 (C9299_24150) | 1..76 | - | 76 | Protein_0 | prepilin | - |
| C9299_RS24105 (C9299_24160) | 390..1676 | - | 1287 | WP_015057162 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| C9299_RS24110 (C9299_24165) | 1689..2324 | - | 636 | WP_000934979 | A24 family peptidase | - |
| C9299_RS24115 (C9299_24170) | 2328..2810 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| C9299_RS24120 (C9299_24175) | 2876..3433 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| C9299_RS24125 (C9299_24180) | 3478..4587 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| C9299_RS24130 (C9299_24185) | 4578..6131 | - | 1554 | WP_000466228 | ATPase, T2SS/T4P/T4SS family | virB11 |
| C9299_RS24135 (C9299_24190) | 6156..6650 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| C9299_RS24140 (C9299_24195) | 6634..7944 | - | 1311 | WP_250205619 | type 4b pilus protein PilO2 | - |
| C9299_RS24145 (C9299_24200) | 7995..9638 | - | 1644 | WP_136758591 | PilN family type IVB pilus formation outer membrane protein | - |
| C9299_RS24150 (C9299_24205) | 9689..11647 | - | 1959 | WP_000338974 | type IV secretory system conjugative DNA transfer family protein | - |
| C9299_RS24155 (C9299_24210) | 11663..12718 | - | 1056 | WP_021575850 | P-type DNA transfer ATPase VirB11 | virB11 |
| C9299_RS24160 (C9299_24215) | 12769..13908 | - | 1140 | WP_000790641 | TrbI/VirB10 family protein | virB10 |
| C9299_RS24165 (C9299_24220) | 13898..14599 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| C9299_RS24170 (C9299_24225) | 14665..15399 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| C9299_RS24180 (C9299_24235) | 15565..17922 | - | 2358 | WP_000548953 | VirB4 family type IV secretion system protein | virb4 |
| C9299_RS24185 (C9299_24240) | 17928..18248 | - | 321 | WP_136758592 | VirB3 family type IV secretion system protein | virB3 |
| C9299_RS24795 | 18319..18609 | - | 291 | WP_000865478 | TrbC/VirB2 family protein | virB2 |
| C9299_RS24195 (C9299_24250) | 18609..19193 | - | 585 | WP_001177118 | lytic transglycosylase domain-containing protein | virB1 |
| C9299_RS24200 (C9299_24255) | 19214..19612 | - | 399 | WP_001153669 | hypothetical protein | - |
| C9299_RS24205 (C9299_24260) | 19731..20168 | - | 438 | WP_100033418 | type IV pilus biogenesis protein PilM | - |
| C9299_RS24210 (C9299_24265) | 20174..21409 | - | 1236 | WP_021575847 | toxin co-regulated pilus biosynthesis Q family protein | - |
| C9299_RS24215 (C9299_24270) | 21412..21699 | - | 288 | WP_001326593 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| C9299_RS24220 (C9299_24275) | 21871..22506 | - | 636 | WP_000835773 | hypothetical protein | - |
| C9299_RS24225 (C9299_24280) | 22579..22866 | - | 288 | WP_001032611 | EexN family lipoprotein | - |
| C9299_RS24230 (C9299_24285) | 22879..23133 | - | 255 | WP_001043555 | EexN family lipoprotein | - |
| C9299_RS24235 (C9299_24290) | 23135..23776 | - | 642 | WP_001425343 | type IV secretion system protein | - |
| C9299_RS24240 (C9299_24295) | 23782..24777 | - | 996 | WP_001028543 | type IV secretion system protein | virB6 |
| C9299_RS24245 (C9299_24300) | 24781..25038 | - | 258 | WP_000739144 | hypothetical protein | - |
| C9299_RS24250 (C9299_24305) | 25035..25337 | - | 303 | WP_001360345 | hypothetical protein | - |
| C9299_RS24255 (C9299_24310) | 25318..25575 | - | 258 | WP_024241063 | hypothetical protein | - |
| C9299_RS24260 (C9299_24315) | 25609..26262 | - | 654 | WP_136758593 | hypothetical protein | - |
| C9299_RS24715 | 26273..26443 | - | 171 | WP_000550721 | hypothetical protein | - |
| C9299_RS24265 (C9299_24320) | 26447..26890 | - | 444 | WP_000498521 | NfeD family protein | - |
| C9299_RS24270 (C9299_24325) | 26952..27905 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| C9299_RS24720 | 27932..28108 | - | 177 | WP_000753050 | hypothetical protein | - |
| C9299_RS24275 (C9299_24330) | 28101..28316 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| C9299_RS24280 (C9299_24335) | 28309..28761 | - | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 2411 | GenBank | NZ_RRRY01000068 |
| Plasmid name | FWSEC0530|unnamed2 | Incompatibility group | IncI2 |
| Plasmid size | 54665 bp | Coordinate of oriT [Strand] | 42346..42399 [-] |
| Host baterium | Escherichia coli strain FWSEC0530 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |