Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101946
Name   oriT_161378|unnamed in_silico
Organism   Salmonella enterica subsp. enterica serovar Orion strain 161378
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_VUJJ01000014 (36397..36485 [+], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_161378|unnamed
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1572 GenBank   WP_001474686
Name   nikB_F1E45_RS21915_161378|unnamed insolico UniProt ID   A0A2J0RB70
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103834.33 Da        Isoelectric Point: 7.3473

>WP_001474686.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFLQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A2J0RB70


Auxiliary protein

ID   637 GenBank   WP_001283947
Name   WP_001283947_161378|unnamed insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   1240 GenBank   WP_001289271
Name   trbC_F1E45_RS21910_161378|unnamed insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86927.12 Da        Isoelectric Point: 6.7713

>WP_001289271.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKIDVQELIELH
PGEFFSIFRGETVPSASFFIPDNEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHLTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1313..31019

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1E45_RS21760 (F1E45_21975) 8..1162 + 1155 WP_001139958 site-specific integrase -
F1E45_RS21765 (F1E45_21980) 1313..2137 + 825 WP_001238927 conjugal transfer protein TraE traE
F1E45_RS21770 (F1E45_21985) 2223..3425 + 1203 WP_000976353 conjugal transfer protein TraF -
F1E45_RS21775 (F1E45_21990) 3485..4069 + 585 WP_000977517 histidine phosphatase family protein -
F1E45_RS21780 (F1E45_21995) 4464..4922 + 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
F1E45_RS21785 (F1E45_22000) 4919..5737 + 819 WP_130570434 IncI1-type conjugal transfer lipoprotein TraI traI
F1E45_RS21790 (F1E45_22005) 5734..6882 + 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
F1E45_RS21795 (F1E45_22010) 6879..7169 + 291 WP_001299214 hypothetical protein traK
F1E45_RS21800 (F1E45_22015) 7184..7735 + 552 WP_000014584 phospholipase D family protein -
F1E45_RS21805 (F1E45_22020) 7825..11592 + 3768 WP_001141535 LPD7 domain-containing protein -
F1E45_RS21810 (F1E45_22025) 11610..11957 + 348 WP_001055900 conjugal transfer protein traL
F1E45_RS21815 (F1E45_22030) 11954..12646 + 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
F1E45_RS21820 (F1E45_22035) 12657..13640 + 984 WP_001191878 IncI1-type conjugal transfer protein TraN traN
F1E45_RS21825 (F1E45_22040) 13643..14932 + 1290 WP_001271997 conjugal transfer protein TraO traO
F1E45_RS21830 (F1E45_22045) 14932..15636 + 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
F1E45_RS21835 (F1E45_22050) 15636..16163 + 528 WP_001055569 conjugal transfer protein TraQ traQ
F1E45_RS21840 (F1E45_22055) 16214..16618 + 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
F1E45_RS21845 (F1E45_22060) 16682..16870 + 189 WP_001277255 putative conjugal transfer protein TraS -
F1E45_RS21850 (F1E45_22065) 16854..17654 + 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
F1E45_RS21855 (F1E45_22070) 17744..20788 + 3045 WP_001024752 IncI1-type conjugal transfer protein TraU traU
F1E45_RS21860 (F1E45_22075) 20788..21402 + 615 WP_000337394 IncI1-type conjugal transfer protein TraV traV
F1E45_RS21865 (F1E45_22080) 21369..22571 + 1203 WP_001189158 IncI1-type conjugal transfer protein TraW traW
F1E45_RS21870 (F1E45_22085) 22600..23184 + 585 WP_001037998 conjugal transfer protein TraX -
F1E45_RS21875 (F1E45_22090) 23281..25449 + 2169 WP_000698368 DotA/TraY family protein traY
F1E45_RS21880 (F1E45_22095) 25520..26182 + 663 WP_000644794 plasmid IncI1-type surface exclusion protein ExcA -
F1E45_RS21885 (F1E45_22100) 26254..26463 - 210 WP_000062603 HEAT repeat domain-containing protein -
F1E45_RS23225 26855..27031 + 177 WP_001054900 hypothetical protein -
F1E45_RS21890 (F1E45_22115) 27955..28206 + 252 WP_001291965 hypothetical protein -
F1E45_RS21895 (F1E45_22120) 28278..28430 - 153 WP_001303307 Hok/Gef family protein -
F1E45_RS21900 (F1E45_22125) 28722..29930 + 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
F1E45_RS21905 (F1E45_22130) 29949..31019 + 1071 WP_000151575 IncI1-type conjugal transfer protein TrbB trbB
F1E45_RS21910 (F1E45_22135) 31012..33303 + 2292 WP_001289271 F-type conjugative transfer protein TrbC -

Host bacterium

ID   2390 GenBank   NZ_VUJJ01000014
Plasmid name   161378|unnamed Incompatibility group   -
Plasmid size   74544 bp Coordinate of oriT [Strand]   36397..36485 [+]
Host baterium   Salmonella enterica subsp. enterica serovar Orion strain 161378

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -