Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101945
Name   oriT_159747|unnamed in_silico
Organism   Salmonella enterica subsp. enterica serovar Muenchen strain 159747
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_VUJE01000010 (27908..27996 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_159747|unnamed
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1571 GenBank   WP_001306778
Name   nikB_F1E44_RS22495_159747|unnamed insolico UniProt ID   Q79VV7
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104010.41 Da        Isoelectric Point: 7.4265

>WP_001306778.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB Q79VV7


Auxiliary protein

ID   636 GenBank   WP_001283947
Name   WP_001283947_159747|unnamed insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   1239 GenBank   WP_001289276
Name   trbC_F1E44_RS22500_159747|unnamed insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 33374..66260

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1E44_RS22500 (F1E44_22880) 31090..33381 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
F1E44_RS22505 (F1E44_22885) 33374..34444 - 1071 WP_000151582 IncI1-type conjugal transfer protein TrbB trbB
F1E44_RS22510 (F1E44_22890) 34463..35671 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
F1E44_RS22515 (F1E44_22895) 35978..36757 - 780 WP_275450201 protein FinQ -
F1E44_RS22520 (F1E44_22900) 37384..37536 + 153 WP_001303307 Hok/Gef family protein -
F1E44_RS22525 (F1E44_22905) 37608..37859 - 252 WP_001291965 hypothetical protein -
F1E44_RS23600 38783..38959 - 177 WP_001054900 hypothetical protein -
F1E44_RS22530 (F1E44_22920) 39168..39377 - 210 Protein_45 hemolysin expression modulator Hha -
F1E44_RS22535 (F1E44_22925) 39475..40089 - 615 WP_000578649 plasmid IncI1-type surface exclusion protein ExcA -
F1E44_RS22540 (F1E44_22930) 40165..42333 - 2169 WP_000698360 IncI1-type conjugal transfer membrane protein TraY traY
F1E44_RS22545 (F1E44_22935) 42430..43014 - 585 WP_001037987 IncI1-type conjugal transfer protein TraX -
F1E44_RS22550 (F1E44_22940) 43043..44245 - 1203 WP_001189159 IncI1-type conjugal transfer protein TraW traW
F1E44_RS22555 (F1E44_22945) 44212..44826 - 615 WP_000337394 IncI1-type conjugal transfer protein TraV traV
F1E44_RS22560 (F1E44_22950) 44826..47870 - 3045 WP_001024752 IncI1-type conjugal transfer protein TraU traU
F1E44_RS22565 (F1E44_22955) 48013..49035 + 1023 WP_000255944 IS21-like element IS100 family transposase -
F1E44_RS22570 (F1E44_22960) 49032..49814 + 783 WP_001300609 IS21-like element IS100kyp family helper ATPase IstB -
F1E44_RS22575 (F1E44_22965) 49919..50719 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
F1E44_RS22580 (F1E44_22970) 50703..50891 - 189 WP_001277255 putative conjugal transfer protein TraS -
F1E44_RS22585 (F1E44_22975) 50955..51359 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
F1E44_RS22590 (F1E44_22980) 51410..51937 - 528 WP_001055569 conjugal transfer protein TraQ traQ
F1E44_RS22595 (F1E44_22985) 51937..52641 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
F1E44_RS22600 (F1E44_22990) 52641..53930 - 1290 WP_001271997 conjugal transfer protein TraO traO
F1E44_RS22605 (F1E44_22995) 53933..54916 - 984 WP_001191878 IncI1-type conjugal transfer protein TraN traN
F1E44_RS22610 (F1E44_23000) 54927..55619 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
F1E44_RS22615 (F1E44_23005) 55616..55963 - 348 WP_001055900 conjugal transfer protein traL
F1E44_RS22620 (F1E44_23010) 55981..59748 - 3768 WP_001141539 LPD7 domain-containing protein -
F1E44_RS22625 (F1E44_23015) 59838..60389 - 552 WP_000014584 phospholipase D family protein -
F1E44_RS22630 (F1E44_23020) 60404..60694 - 291 WP_001299214 hypothetical protein traK
F1E44_RS22635 (F1E44_23025) 60691..61839 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
F1E44_RS22640 (F1E44_23030) 61836..62654 - 819 WP_000646097 IncI1-type conjugal transfer lipoprotein TraI traI
F1E44_RS22645 (F1E44_23035) 62651..63109 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
F1E44_RS22650 (F1E44_23040) 63504..64088 - 585 WP_000977522 histidine phosphatase family protein -
F1E44_RS22655 (F1E44_23045) 64148..65350 - 1203 WP_000976353 conjugal transfer protein TraF -
F1E44_RS22660 (F1E44_23050) 65436..66260 - 825 WP_001238927 conjugal transfer protein TraE traE
F1E44_RS22665 (F1E44_23055) 66411..67565 - 1155 WP_001139958 site-specific integrase -

Host bacterium

ID   2389 GenBank   NZ_VUJE01000010
Plasmid name   159747|unnamed Incompatibility group   -
Plasmid size   67572 bp Coordinate of oriT [Strand]   27908..27996 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Muenchen strain 159747

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrVA2