Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   101942
Name   oriT_159196|unnamed1 contig029 in_silico
Organism   Salmonella enterica subsp. enterica serovar Java strain 159196
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_VUJC01000029 (2697..3303 [-], 607 nt)
oriT length   607 nt
IRs (inverted repeats)      590..595, 600..605  (ATGCCC..GGGCAT)
 517..523, 529..535  (CCTCCCG..CGGGAGG)
 453..458, 462..467  (GTTCGC..GCGAAC)
 196..202, 204..210  (TCAATTC..GAATTGA)
 175..180, 187..192  (AAATCA..TGATTT)
 79..84, 93..98  (GCTTTA..TAAAGC)
 29..37, 46..54  (TTTTGATAA..TTATCAAAA)
Location of nic site      313..314
Conserved sequence flanking the
  nic site  
 
 TCCTGCATCG
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 607 nt

>oriT_159196|unnamed1 contig029
CTTTGTTTACCTGTTAAGTACATCGTTGTTTTGATAAAATCATTATTATCAAAAAACGTATTTATATCCTTACTGCCAGCTTTACTTTTTAATAAAGCATTTGCCAAAAGTTTCATGCCTTTAGCTACTGTTAAAGCTGGTTCATCTGGATAAAGAGTTTTAAGGCAATCCAGCAAATCATCATGCTGATTTTCATCAATTCTGAATTGAACTTTTTTCATAAAATTCTCCAAAAAGAACCGACTGTAGGTCACCGGGCAAACGTTGCGGAATGGCGTCAGAGACGTCATTTTGCGGCGTTTGCCCTATCCTGCATCGCAGTGAATCTGGCGCCGCTCATAAATTGTGCTTGAGCATCAACAAAAATGCAAAAAGGCTGATGTTGTCATGGCTGAACATACGTACGTCTGCGACCGGAAACCCGATCAATGCGGTAAGGACAGTGCCGTCAGGTTCGCTCCGCGAACGGTCTGGCAGAAAGCGCAAGCGCAGCCCCGCGCAATATATCAGAAAGCACCTCCCGAAATACGGGAGGGCTTCGGCTATTCAGCTAAGGTTGCTCTGTTCAGGTTGCTGAGTGTAGCCTTCCATGCCCTGACGGGCATCA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   1569 GenBank   WP_000539538
Name   mobP1_F1E38_RS22535_159196|unnamed1 contig029 insolico UniProt ID   A0A5J2A6K9
Length   388 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 388 a.a.        Molecular weight: 44507.83 Da        Isoelectric Point: 10.5067

>WP_000539538.1 MULTISPECIES: MobP1 family relaxase [Enterobacterales]
MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAVTRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKDHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDVGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GDLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFWSL

  Protein domains


Predicted by InterproScan.

(70-229)


  Protein structure


Source ID Structure
AlphaFold DB A0A5J2A6K9


Host bacterium


ID   2386 GenBank   NZ_VUJC01000029
Plasmid name   159196|unnamed1 contig029 Incompatibility group   -
Plasmid size   11611 bp Coordinate of oriT [Strand]   2697..3303 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Java strain 159196

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -