Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101941
Name   oriT_161403|unnamed in_silico
Organism   Salmonella enterica subsp. enterica serovar Muenchen strain 161403
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_VUJK01000014 (38060..38148 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_161403|unnamed
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1568 GenBank   WP_001474686
Name   nikB_F1E42_RS22015_161403|unnamed insolico UniProt ID   A0A2J0RB70
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103834.33 Da        Isoelectric Point: 7.3473

>WP_001474686.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFLQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A2J0RB70


Auxiliary protein

ID   635 GenBank   WP_001283947
Name   WP_001283947_161403|unnamed insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   1238 GenBank   WP_001289271
Name   trbC_F1E42_RS22020_161403|unnamed insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86927.12 Da        Isoelectric Point: 6.7713

>WP_001289271.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKIDVQELIELH
PGEFFSIFRGETVPSASFFIPDNEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHLTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 43526..73232

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1E42_RS22020 (F1E42_22255) 41242..43533 - 2292 WP_001289271 F-type conjugative transfer protein TrbC -
F1E42_RS22025 (F1E42_22260) 43526..44596 - 1071 WP_000151575 IncI1-type conjugal transfer protein TrbB trbB
F1E42_RS22030 (F1E42_22265) 44615..45823 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
F1E42_RS22035 (F1E42_22270) 46115..46267 + 153 WP_001303307 Hok/Gef family protein -
F1E42_RS22040 (F1E42_22275) 46339..46590 - 252 WP_001291965 hypothetical protein -
F1E42_RS23235 47514..47690 - 177 WP_001054900 hypothetical protein -
F1E42_RS22045 (F1E42_22290) 48082..48291 + 210 WP_000062603 HEAT repeat domain-containing protein -
F1E42_RS22050 (F1E42_22295) 48363..49025 - 663 WP_000644794 plasmid IncI1-type surface exclusion protein ExcA -
F1E42_RS22055 (F1E42_22300) 49096..51264 - 2169 WP_000698368 DotA/TraY family protein traY
F1E42_RS22060 (F1E42_22305) 51361..51945 - 585 WP_001037998 conjugal transfer protein TraX -
F1E42_RS22065 (F1E42_22310) 51974..53176 - 1203 WP_001189158 IncI1-type conjugal transfer protein TraW traW
F1E42_RS22070 (F1E42_22315) 53143..53757 - 615 WP_000337394 IncI1-type conjugal transfer protein TraV traV
F1E42_RS22075 (F1E42_22320) 53757..56801 - 3045 WP_001024752 IncI1-type conjugal transfer protein TraU traU
F1E42_RS22080 (F1E42_22325) 56891..57691 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
F1E42_RS22085 (F1E42_22330) 57675..57863 - 189 WP_001277255 putative conjugal transfer protein TraS -
F1E42_RS22090 (F1E42_22335) 57927..58331 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
F1E42_RS22095 (F1E42_22340) 58382..58909 - 528 WP_001055569 conjugal transfer protein TraQ traQ
F1E42_RS22100 (F1E42_22345) 58909..59613 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
F1E42_RS22105 (F1E42_22350) 59613..60902 - 1290 WP_001271997 conjugal transfer protein TraO traO
F1E42_RS22110 (F1E42_22355) 60905..61888 - 984 WP_001191878 IncI1-type conjugal transfer protein TraN traN
F1E42_RS22115 (F1E42_22360) 61899..62591 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
F1E42_RS22120 (F1E42_22365) 62588..62935 - 348 WP_001055900 conjugal transfer protein traL
F1E42_RS22125 (F1E42_22370) 62953..66720 - 3768 WP_001141535 LPD7 domain-containing protein -
F1E42_RS22130 (F1E42_22375) 66810..67361 - 552 WP_000014584 phospholipase D family protein -
F1E42_RS22135 (F1E42_22380) 67376..67666 - 291 WP_001299214 hypothetical protein traK
F1E42_RS22140 (F1E42_22385) 67663..68811 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
F1E42_RS22145 (F1E42_22390) 68808..69626 - 819 WP_130570434 IncI1-type conjugal transfer lipoprotein TraI traI
F1E42_RS22150 (F1E42_22395) 69623..70081 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
F1E42_RS22155 (F1E42_22400) 70476..71060 - 585 WP_000977517 histidine phosphatase family protein -
F1E42_RS22160 (F1E42_22405) 71120..72322 - 1203 WP_000976353 conjugal transfer protein TraF -
F1E42_RS22165 (F1E42_22410) 72408..73232 - 825 WP_001238927 conjugal transfer protein TraE traE
F1E42_RS22170 (F1E42_22415) 73383..74537 - 1155 WP_001139958 site-specific integrase -
F1E42_RS23635 74855..75140 - 286 Protein_82 Shufflon protein B -

Host bacterium

ID   2385 GenBank   NZ_VUJK01000014
Plasmid name   161403|unnamed Incompatibility group   -
Plasmid size   75140 bp Coordinate of oriT [Strand]   38060..38148 [-]
Host baterium   Salmonella enterica subsp. enterica serovar Muenchen strain 161403

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -