Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101936 |
| Name | oriT_pKUFSE-SAL43-1  |
| Organism | Salmonella enterica subsp. enterica serovar Dessau strain KUFSE-CM43 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JAALJB010000002 (43445..44051 [+], 607 nt) |
| oriT length | 607 nt |
| IRs (inverted repeats) | 590..595, 600..605 (ATGCCC..GGGCAT) 517..523, 529..535 (CCTCCCG..CGGGAGG) 453..458, 462..467 (GTTCGC..GCGAAC) 196..202, 204..210 (TCAATTC..GAATTGA) 175..180, 187..192 (AAATCA..TGATTT) 103..108, 120..125 (GCTAAA..TTTAGC) 79..84, 93..98 (GCTTTA..TAAAGC) 29..37, 46..54 (TTTTGATAA..TTATCAAAA) |
| Location of nic site | 313..314 |
| Conserved sequence flanking the nic site |
TCCTGCATCG |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 607 nt
>oriT_pKUFSE-SAL43-1
CTTTGTTTACCTGTTAAGTACATCGTTGTTTTGATAAAATCATTATTATCAAAAAACGTATTTATATCCTTACTGACAGCTTTACTTTTTAATAAAGCATTTGCTAAAAGTTTCATGCCTTTAGCTACTGTTAAAGCTGGTTCATCCGGATAAAGAGTTTTAAGGCAATCCAGCAAATCATCATGCTGATTTTCATCAATTCTGAATTGAACTTTTTTCATAAAATTCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAATGTTGCGGAATGGCGTCAGAGACGTCATTTTGCGGCGTTTGCCCTATCCTGCATCGCAGTGAATCTGGCGCCGCTCATAAATTGTGCTTGAGCATCAACAAAAATGCAAAAAGGCTGATGTTGTCATGGCTGAACATACGTACGTCTGCGACCGGAAACCCGATCAATGCGGTAAGGACAGTGCCGTCAGGTTCGCTCCGCGAACGGTCTGGCAGGAAGCGCAAGCGCAGTCCCGCGCAATATATCAGAAAGCACCTCCCGAAATACGGGAGGGCTTCGGCTATTCAGCTAAGGTTGCTCTGTTCAGGTTGCTGAGTGTAGCCTTCCATGCCCTGACGGGCATCA
CTTTGTTTACCTGTTAAGTACATCGTTGTTTTGATAAAATCATTATTATCAAAAAACGTATTTATATCCTTACTGACAGCTTTACTTTTTAATAAAGCATTTGCTAAAAGTTTCATGCCTTTAGCTACTGTTAAAGCTGGTTCATCCGGATAAAGAGTTTTAAGGCAATCCAGCAAATCATCATGCTGATTTTCATCAATTCTGAATTGAACTTTTTTCATAAAATTCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAATGTTGCGGAATGGCGTCAGAGACGTCATTTTGCGGCGTTTGCCCTATCCTGCATCGCAGTGAATCTGGCGCCGCTCATAAATTGTGCTTGAGCATCAACAAAAATGCAAAAAGGCTGATGTTGTCATGGCTGAACATACGTACGTCTGCGACCGGAAACCCGATCAATGCGGTAAGGACAGTGCCGTCAGGTTCGCTCCGCGAACGGTCTGGCAGGAAGCGCAAGCGCAGTCCCGCGCAATATATCAGAAAGCACCTCCCGAAATACGGGAGGGCTTCGGCTATTCAGCTAAGGTTGCTCTGTTCAGGTTGCTGAGTGTAGCCTTCCATGCCCTGACGGGCATCA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1566 | GenBank | WP_000539529 |
| Name | mobP1_G6Z41_RS23410_pKUFSE-SAL43-1 |
UniProt ID | A0A0F7JE92 |
| Length | 388 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 388 a.a. Molecular weight: 44493.85 Da Isoelectric Point: 10.5286
>WP_000539529.1 MULTISPECIES: MobP1 family relaxase [Enterobacteriaceae]
MGVYVDKEHRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAITRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GDLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFWGL
MGVYVDKEHRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGAITRQGIRNSI
DYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENKKITQNIVFSPPVSAKVKPED
LLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYD
VKATHKQQHGLNQSVKDAHNTAPKRQKGVYEVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADF
GDLCSRESVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLN
SPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFWGL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0F7JE92 |
T4CP
| ID | 1237 | GenBank | WP_024127754 |
| Name | t4cp2_G6Z41_RS23345_pKUFSE-SAL43-1 |
UniProt ID | _ |
| Length | 611 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 611 a.a. Molecular weight: 69402.32 Da Isoelectric Point: 7.7563
>WP_024127754.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKISPKLASLTMKLNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREILLGNKVYLLDPFNSKTHQFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSI
GSVVDLYSNIDRELILSKREELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAAKIS
EKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEEFIILKGENPVKAEKALYYLDPY
FMDRLMKISPKLASLTMKLNKTKKIFGVKGLKYPSKEKMLSVGELESEVLL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 30888..40905
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| G6Z41_RS23325 (G6Z41_23350) | 25898..26104 | - | 207 | WP_000850859 | hemolysin expression modulator Hha | - |
| G6Z41_RS23330 (G6Z41_23355) | 26101..28254 | - | 2154 | WP_024127756 | type IA DNA topoisomerase | - |
| G6Z41_RS23335 (G6Z41_23360) | 28341..28643 | - | 303 | WP_000717627 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| G6Z41_RS23340 (G6Z41_23365) | 28640..29053 | - | 414 | WP_024127755 | cag pathogenicity island Cag12 family protein | - |
| G6Z41_RS23345 (G6Z41_23370) | 29050..30885 | - | 1836 | WP_024127754 | type IV secretory system conjugative DNA transfer family protein | - |
| G6Z41_RS23350 (G6Z41_23375) | 30888..31919 | - | 1032 | WP_052921381 | P-type DNA transfer ATPase VirB11 | virB11 |
| G6Z41_RS23355 (G6Z41_23380) | 31921..33126 | - | 1206 | WP_052921380 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| G6Z41_RS23360 (G6Z41_23385) | 33123..34052 | - | 930 | WP_024127752 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| G6Z41_RS23365 (G6Z41_23390) | 34057..34770 | - | 714 | WP_000394613 | type IV secretion system protein | virB8 |
| G6Z41_RS23370 (G6Z41_23395) | 35016..36146 | - | 1131 | WP_024127751 | type IV secretion system protein | virB6 |
| G6Z41_RS23375 (G6Z41_23400) | 36155..36442 | - | 288 | WP_000835347 | EexN family lipoprotein | - |
| G6Z41_RS23380 (G6Z41_23405) | 36443..37201 | - | 759 | WP_000744200 | type IV secretion system protein | - |
| G6Z41_RS23385 (G6Z41_23410) | 37212..39965 | - | 2754 | WP_064203856 | VirB3 family type IV secretion system protein | virb4 |
| G6Z41_RS23390 (G6Z41_23415) | 39984..40280 | - | 297 | WP_000916182 | TrbC/VirB2 family protein | virB2 |
| G6Z41_RS23395 (G6Z41_23420) | 40258..40905 | - | 648 | WP_000953529 | lytic transglycosylase domain-containing protein | virB1 |
| G6Z41_RS23400 (G6Z41_23425) | 40938..41150 | - | 213 | WP_000125172 | hypothetical protein | - |
| G6Z41_RS23405 (G6Z41_23430) | 41080..41598 | - | 519 | WP_024127747 | transcription termination/antitermination NusG family protein | - |
| G6Z41_RS23410 (G6Z41_23435) | 41951..43117 | - | 1167 | WP_000539529 | MobP1 family relaxase | - |
| G6Z41_RS23415 (G6Z41_23440) | 43120..43665 | - | 546 | WP_000757693 | DNA distortion polypeptide 1 | - |
| G6Z41_RS23425 (G6Z41_23445) | 43991..44263 | - | 273 | WP_000160399 | hypothetical protein | - |
| G6Z41_RS23430 (G6Z41_23450) | 44276..44425 | - | 150 | WP_000003880 | hypothetical protein | - |
| G6Z41_RS23435 (G6Z41_23455) | 44495..44674 | - | 180 | WP_000439078 | hypothetical protein | - |
| G6Z41_RS23440 (G6Z41_23460) | 44717..45046 | - | 330 | WP_000866648 | hypothetical protein | - |
| G6Z41_RS23445 (G6Z41_23465) | 45139..45354 | - | 216 | WP_052921379 | hypothetical protein | - |
| G6Z41_RS23450 (G6Z41_23470) | 45344..45589 | - | 246 | WP_001550454 | hypothetical protein | - |
Host bacterium
| ID | 2380 | GenBank | NZ_JAALJB010000002 |
| Plasmid name | pKUFSE-SAL43-1 | Incompatibility group | IncX1 |
| Plasmid size | 47686 bp | Coordinate of oriT [Strand] | 43445..44051 [+] |
| Host baterium | Salmonella enterica subsp. enterica serovar Dessau strain KUFSE-CM43 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |