Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101933
Name   oriT_B199|unnamed 1 in_silico
Organism   Klebsiella pneumoniae strain B199
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_LJCB01000027 (2053..2102 [-], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_B199|unnamed 1
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1564 GenBank   WP_057775027
Name   traI_AFK73_RS25955_B199|unnamed 1 insolico UniProt ID   _
Length   1753 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1753 a.a.        Molecular weight: 190438.65 Da        Isoelectric Point: 6.3863

>WP_057775027.1 conjugative transfer relaxase/helicase TraI [Klebsiella pneumoniae]
MMSIGSVKSADKAADYYTNKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRTQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADSRAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGAAGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGLESVAQISGTREQSVLNGLIRDSLRNEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMESWDPETRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAV
DSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATAMETGGGKVPMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADIAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPAQEKAIQ
KALSEGKTLPAGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHGARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQMITLTDSEGKERYISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDLERGYVANSIWEVQSVAGDSVTLSDGKTTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILE
PRNDRAVKSADLLFGRARLLDETAAGRAALQQSGLAQGNSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNDAARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
VDRVKEVIGDVIRGLERDRPGPEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(11-284)

AAA domain (AAA_30)

(971-1157)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(635-714)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(577-629)

DNA helicase TraI (TraI_C)

(1433-1555)


  Protein structure



No available structure.




T4CP

ID   1235 GenBank   WP_032436737
Name   traD_AFK73_RS25950_B199|unnamed 1 insolico UniProt ID   _
Length   769 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 769 a.a.        Molecular weight: 85553.49 Da        Isoelectric Point: 5.0065

>WP_032436737.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Klebsiella]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREGRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPDDAGSHAGERPELASQPA
PAEVTVSPAPVKAPATTTMPAAEPSARTAEPPVLRVTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1495..30102

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFK73_RS25770 (AFK73_25870) 279..1100 + 822 WP_004152492 DUF932 domain-containing protein -
AFK73_RS30335 1133..1462 + 330 WP_011977736 DUF5983 family protein -
AFK73_RS25775 (AFK73_25875) 1495..1980 - 486 WP_004178063 transglycosylase SLT domain-containing protein virB1
AFK73_RS25780 (AFK73_25880) 2414..2806 + 393 WP_004178062 conjugal transfer relaxosome DNA-binding protein TraM -
AFK73_RS30340 3020..3715 + 696 WP_004178061 transcriptional regulator TraJ family protein -
AFK73_RS25790 (AFK73_25890) 3799..4170 + 372 WP_004208838 TraY domain-containing protein -
AFK73_RS25795 (AFK73_25895) 4224..4592 + 369 WP_004178060 type IV conjugative transfer system pilin TraA -
AFK73_RS25800 (AFK73_25900) 4606..4911 + 306 WP_004178059 type IV conjugative transfer system protein TraL traL
AFK73_RS25805 (AFK73_25905) 4931..5497 + 567 WP_004152602 type IV conjugative transfer system protein TraE traE
AFK73_RS25810 (AFK73_25910) 5484..6224 + 741 WP_004152601 type-F conjugative transfer system secretin TraK traK
AFK73_RS25815 (AFK73_25915) 6224..7648 + 1425 WP_004152600 F-type conjugal transfer pilus assembly protein TraB traB
AFK73_RS25820 (AFK73_25920) 7641..8237 + 597 WP_004152599 conjugal transfer pilus-stabilizing protein TraP -
AFK73_RS25825 (AFK73_25925) 8260..8829 + 570 WP_004152598 type IV conjugative transfer system lipoprotein TraV traV
AFK73_RS25830 (AFK73_25930) 8961..9371 + 411 WP_004152597 lipase chaperone -
AFK73_RS31025 (AFK73_25935) 9376..9666 + 291 WP_032423381 hypothetical protein -
AFK73_RS25840 (AFK73_25940) 9690..9908 + 219 WP_004171484 hypothetical protein -
AFK73_RS25845 (AFK73_25945) 9909..10247 + 339 WP_032436750 hypothetical protein -
AFK73_RS25850 (AFK73_25950) 10292..10696 + 405 WP_023307543 hypothetical protein -
AFK73_RS25855 (AFK73_25955) 10693..10983 + 291 WP_023307542 hypothetical protein -
AFK73_RS25860 (AFK73_25960) 10991..11389 + 399 WP_072158599 hypothetical protein -
AFK73_RS25865 (AFK73_25965) 11461..14100 + 2640 WP_016528865 type IV secretion system protein TraC virb4
AFK73_RS25870 (AFK73_25970) 14100..14489 + 390 WP_032436749 type-F conjugative transfer system protein TrbI -
AFK73_RS25875 (AFK73_25975) 14489..15115 + 627 WP_023307538 type-F conjugative transfer system protein TraW traW
AFK73_RS25880 (AFK73_25980) 15159..16118 + 960 WP_015065634 conjugal transfer pilus assembly protein TraU traU
AFK73_RS25885 (AFK73_25985) 16131..16769 + 639 WP_015065635 type-F conjugative transfer system pilin assembly protein TrbC trbC
AFK73_RS25890 (AFK73_25990) 16828..18783 + 1956 WP_057775023 type-F conjugative transfer system mating-pair stabilization protein TraN traN
AFK73_RS25895 (AFK73_25995) 18815..19051 + 237 WP_032436746 conjugal transfer protein TrbE -
AFK73_RS32860 (AFK73_26000) 19048..19233 + 186 WP_032436745 hypothetical protein -
AFK73_RS25905 (AFK73_26005) 19279..19605 + 327 WP_032436744 hypothetical protein -
AFK73_RS25910 (AFK73_26010) 19626..20378 + 753 WP_015065637 type-F conjugative transfer system pilin assembly protein TraF traF
AFK73_RS25915 (AFK73_26015) 20389..20628 + 240 WP_004144400 type-F conjugative transfer system pilin chaperone TraQ -
AFK73_RS25920 (AFK73_26020) 20600..21157 + 558 WP_004152678 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
AFK73_RS25925 (AFK73_26025) 21202..21645 + 444 WP_032436741 F-type conjugal transfer protein TrbF -
AFK73_RS25930 (AFK73_26030) 21623..23002 + 1380 WP_072158600 conjugal transfer pilus assembly protein TraH traH
AFK73_RS25935 (AFK73_26035) 23002..25824 + 2823 WP_023320108 conjugal transfer mating-pair stabilization protein TraG traG
AFK73_RS25940 (AFK73_26040) 25848..26450 + 603 WP_023280878 hypothetical protein -
AFK73_RS25945 (AFK73_26045) 26702..27433 + 732 WP_032415736 conjugal transfer complement resistance protein TraT -
AFK73_RS32640 27624..27737 + 114 Protein_37 hypothetical protein -
AFK73_RS25950 (AFK73_26050) 27793..30102 + 2310 WP_032436737 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   2377 GenBank   NZ_LJCB01000027
Plasmid name   B199|unnamed 1 Incompatibility group   -
Plasmid size   49781 bp Coordinate of oriT [Strand]   2053..2102 [-]
Host baterium   Klebsiella pneumoniae strain B199

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -