Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101929
Name   oriT_pRIH79 in_silico
Organism   Enterococcus faecium C68
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_LPUE01000005 (34263..34454 [+], 192 nt)
oriT length   192 nt
IRs (inverted repeats)      106..112, 122..128  (ATTTTTT..AAAAAAT)
 107..113, 120..126  (TTTTTTG..CAAAAAA)
 93..98, 105..110  (AAAATA..TATTTT)
 65..71, 75..81  (AGTTGGC..GCCAACT)
 41..49, 52..60  (CACCTTCCT..AGGAAGGTG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 192 nt

>oriT_pRIH79
CCAAGGAATTAATACAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATAGCTTCATATTTTTTGCTATCACAAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1561 GenBank   WP_002326870
Name   mobP2_AU251_RS15330_pRIH79 insolico UniProt ID   B5U8V6
Length   506 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 506 a.a.        Molecular weight: 60597.01 Da        Isoelectric Point: 9.3485

>WP_002326870.1 MULTISPECIES: MobP2 family relaxase [Enterococcus]
MTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN

  Protein domains


Predicted by InterproScan.

MobL relaxases (MobL)

(11-438)


  Protein structure


Source ID Structure
AlphaFold DB B5U8V6


T4CP

ID   1233 GenBank   WP_002311855
Name   t4cp2_AU251_RS15390_pRIH79 insolico UniProt ID   _
Length   952 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 952 a.a.        Molecular weight: 108225.12 Da        Isoelectric Point: 7.4751

>WP_002311855.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQ
IMILEQQLANAYELAVFKTKNSILKNKIIKLKNAMTKLAKAI

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(199-442)

TraM recognition site of TraD and TraG (TraG-D_C)

(589-701)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 25783..53284

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AU251_RS15250 (AU251_15285) 20937..21125 + 189 WP_002305130 type II toxin-antitoxin system HicA family toxin -
AU251_RS15255 (AU251_15290) 21171..21548 + 378 WP_002305129 type II toxin-antitoxin system HicB family antitoxin -
AU251_RS15260 (AU251_15295) 21779..22951 - 1173 WP_002311882 hypothetical protein -
AU251_RS15265 (AU251_15300) 23195..24163 - 969 WP_002311881 zeta toxin family protein -
AU251_RS15270 (AU251_15305) 24160..24357 - 198 WP_002311880 hypothetical protein -
AU251_RS15275 (AU251_15310) 24368..24685 - 318 WP_002311879 hypothetical protein -
AU251_RS15280 (AU251_15315) 24722..24940 - 219 WP_002311878 hypothetical protein -
AU251_RS15285 (AU251_15320) 25049..25240 - 192 WP_002311877 hypothetical protein -
AU251_RS15290 (AU251_15325) 25402..25758 - 357 WP_002311876 hypothetical protein -
AU251_RS15295 (AU251_15330) 25783..26787 - 1005 WP_002311875 DUF3991 domain-containing protein traP
AU251_RS15300 (AU251_15335) 26824..27084 - 261 WP_002311874 hypothetical protein -
AU251_RS15305 (AU251_15340) 27093..28166 - 1074 WP_002311873 hypothetical protein -
AU251_RS15310 (AU251_15345) 28174..30000 - 1827 WP_002311872 ArdC-like ssDNA-binding domain-containing protein -
AU251_RS15315 (AU251_15350) 30155..31021 - 867 WP_002311871 hypothetical protein -
AU251_RS15320 (AU251_15355) 31196..31459 - 264 WP_002311870 hypothetical protein -
AU251_RS15325 (AU251_15360) 31461..31790 - 330 WP_002311869 hypothetical protein -
AU251_RS15330 (AU251_15365) 31812..33332 - 1521 WP_002326870 MobP2 family relaxase -
AU251_RS15335 (AU251_15370) 33414..33881 - 468 WP_002311867 hypothetical protein -
AU251_RS15340 (AU251_15375) 33878..34174 - 297 WP_002311865 hypothetical protein -
AU251_RS15345 (AU251_15380) 34525..34755 - 231 WP_002311864 hypothetical protein -
AU251_RS15350 (AU251_15385) 34791..35687 - 897 WP_002311863 hypothetical protein -
AU251_RS15355 (AU251_15390) 35701..36351 - 651 WP_002311862 cell division protein FtsL -
AU251_RS15360 (AU251_15395) 36366..37502 - 1137 WP_002311861 bifunctional lytic transglycosylase/C40 family peptidase orf14
AU251_RS15365 (AU251_15400) 37524..39479 - 1956 WP_002311860 tra protein virb4
AU251_RS15370 (AU251_15405) 39495..39962 - 468 WP_002311859 hypothetical protein -
AU251_RS15375 (AU251_15410) 39978..40595 - 618 WP_002311858 hypothetical protein virb4
AU251_RS15380 (AU251_15415) 40592..40903 - 312 WP_002317413 DUF5592 family protein -
AU251_RS15385 (AU251_15420) 40951..43620 - 2670 WP_002326868 pLS20_p028 family conjugation system transmembrane protein -
AU251_RS15390 (AU251_15425) 43638..46496 - 2859 WP_002311855 VirD4-like conjugal transfer protein, CD1115 family -
AU251_RS15395 (AU251_15430) 46509..47225 - 717 WP_002311854 hypothetical protein -
AU251_RS15400 (AU251_15435) 47238..49064 - 1827 WP_002311853 hypothetical protein -
AU251_RS15405 (AU251_15440) 49173..49448 - 276 WP_002311852 TrbC/VirB2 family protein virB2
AU251_RS15410 (AU251_15445) 49467..49688 - 222 WP_002326867 hypothetical protein -
AU251_RS17750 49700..49846 - 147 WP_002326866 hypothetical protein -
AU251_RS15415 (AU251_15450) 49860..50222 - 363 WP_002311851 DUF4320 family protein -
AU251_RS15420 (AU251_15455) 50240..51037 - 798 WP_002311850 hypothetical protein -
AU251_RS15425 (AU251_15460) 51037..51852 - 816 WP_002313315 hypothetical protein -
AU251_RS15430 (AU251_15465) 51869..53284 - 1416 WP_002311848 CpaF/VirB11 family protein virB11
AU251_RS15435 (AU251_15470) 53296..53718 - 423 WP_002311847 hypothetical protein -
AU251_RS17755 53786..53938 - 153 WP_002311846 hypothetical protein -
AU251_RS15440 (AU251_15475) 53952..54314 - 363 WP_002311845 hypothetical protein -
AU251_RS15445 (AU251_15480) 54374..58003 - 3630 WP_002311844 SpaA isopeptide-forming pilin-related protein -

Host bacterium

ID   2373 GenBank   NZ_LPUE01000005
Plasmid name   pRIH79 Incompatibility group   -
Plasmid size   78286 bp Coordinate of oriT [Strand]   34263..34454 [+]
Host baterium   Enterococcus faecium C68

Cargo genes

Drug resistance gene   dfrG, aac(6')-aph(2'')
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA1