Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 101900
• Name: oriT_FWSEC0423|unnamed10
• Organism: Escherichia coli strain FWSEC0423
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_RROS01000138 (9526..9598 [+], 73 nt)
• oriT length: 73 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 73 nt

>oriT_FWSEC0423|unnamed10
GTCGGGGCAAAGCCCTGACCAGGTGGCATTTGTCTGATTGCGCGTGCGCGGTCCGACAAATGCACATCCTGTC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_C9198_RS27585_FWSEC0423|unnamed10

• ID: 1553
• GenBank: WP_062891657
• Name: Relaxase_C9198_RS27585_FWSEC0423|unnamed10
• UniProt ID: _
• Length: 906 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 906 a.a.
• Molecular weight: 105092.43 Da
• Isoelectric Point: 8.3694

>WP_062891657.1 relaxase/mobilization nuclease domain-containing protein [Escherichia coli]
MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDVPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWINFYGVTCFHNCNSLETAAEEMQYKADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPVSGHINRLSWSQERLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRHFAERLPKMARNGELKSCLDVHRTLAEAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVIPGERFRGHSIGTQPVNDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
ALIQCRSQIEEMIAGGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAAADIFERVKPECRYNPELAASDEVEPGFRRDPELRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAHIRVQFRDPQVRKLHYHIAEVQRMQALIRLKENVREERLALMAEGKWYP
LSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRTTNADRCVVLCEPGGTPLFNNVAKLEARLQK
NGSVHFRDTRTGKNVCTDYGDRVVFYHHNDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM
VAWHNAAGITGNGHFTISRPDVDLHRQRSEEYYHEYIRQQTRLSESHDENYSSRQENTWVPPSPGM

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (62-315)

Protein structure

No available structure.

Auxiliary protein

WP_001291058_FWSEC0423|unnamed10

• ID: 628
• GenBank: WP_001291058
• Name: WP_001291058_FWSEC0423|unnamed10
• UniProt ID: A0A7I0L241
• Length: 110 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 110 a.a.
• Molecular weight: 12730.52 Da
• Isoelectric Point: 9.9656

>WP_001291058.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVSTFIRSSSLDKVVNCRTDDRMIDEIMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITEYVNALRRDLMGR

Protein domains

No domain identified.

Protein structure

• AlphaFold DB: A0A7I0L241

T4CP

t4cp2_C9198_RS27570_FWSEC0423|unnamed10

• ID: 1227
• GenBank: WP_169072845
• Name: t4cp2_C9198_RS27570_FWSEC0423|unnamed10
• UniProt ID: _
• Length: 422 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 422 a.a.
• Molecular weight: 47602.68 Da
• Isoelectric Point: 5.5539

>WP_169072845.1 TraM recognition domain-containing protein, partial [Escherichia coli]
SGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSALGRMFVTQQSMILARD
LGYRLEGTDAQTLEVKKYKGRFPYICILDEVGAYYTDRIAVEATQVRSLEFSLIMMAQDQERIEGQTSAT
NTATLMQNAGTKFAGRIVSDDKTARTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQ
DLIRLNAGEFFTVFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
HVSSIIGVLTEKTSRKRRKKHTEPYRIIDTFQDQLKRTQTSLDLLPQYDTDIESRANELWKKAVHTINNT
TREERRVCYITLNRPEEEYSGPEDIPSVKEILNTLLPLEILLPVAEKNKSQTINNGHPQKSYGAQPTTDE
KY

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (95-184)

Protein structure

No available structure.

Host bacterium

• ID: 2344
• GenBank: NZ_RROS01000138
• Plasmid name: FWSEC0423|unnamed10
• Incompatibility group: -
• Plasmid size: 10028 bp
• Coordinate of oriT [Strand]: 9526..9598 [+]
• Host baterium: Escherichia coli strain FWSEC0423

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -