Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 101864
• Name: oriT_pNRRL_B23150
• Organism: Bacillus thuringiensis serovar higo strain NRRL B-23150
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_PGSA01000424 (4555..4647 [-], 93 nt)
• oriT length: 93 nt
• IRs (inverted repeats): 66..71, 75..80 (AATTTC..GAAATT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 93 nt

>oriT_pNRRL_B23150
TCAGCTGAAACGTTGCGAATGAGTGTGCCAAACTTCGTAAAGAAGAAGGCACAAGGCAGTCGTTTAATTTCTCCGAAATTTGATAAGGAGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_C4B07_RS33310_pNRRL_B23150

• ID: 1539
• GenBank: WP_088115536
• Name: Relaxase_C4B07_RS33310_pNRRL_B23150
• UniProt ID: _
• Length: 326 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 326 a.a.
• Molecular weight: 37171.54 Da
• Isoelectric Point: 9.8024

>WP_088115536.1 relaxase/mobilization nuclease domain-containing protein [Bacillus thuringiensis]
MATTKLGNTKSASKTINYAEKRAVEKSGHNCDVEYAKSNFKELRMLYGKDKGVQAHTIIQSFKPGEITPE
EANSIGLELAQSIAKDYQVVIYTHTDTEHIHNHIVINSVNIETGKKYHSNNKQRDLIKSENDRICQARGL
SVVVEKQASVRYTAAEKSLLEKGKTSWKDEIRQAVDIAKTRTSDVKGLTAFLDNLGIETRLRGETISYKH
PEALKWVRGSKLGYDYEIGGMERGFERQIGEGKTRTQSINGNEYKRTNQLSSENVGDRTSERTDCATYRP
ISRKYREEQQARERANTLRQQNEQKKQRELEQQSRAIERSGPSLER

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (8-238)

Protein structure

No available structure.

Host bacterium

• ID: 2308
• GenBank: NZ_PGSA01000424
• Plasmid name: pNRRL_B23150
• Incompatibility group: -
• Plasmid size: 13094 bp
• Coordinate of oriT [Strand]: 4555..4647 [-]
• Host baterium: Bacillus thuringiensis serovar higo strain NRRL B-23150

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -