Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 101863
• Name: oriT_FWSEC0390|unnamed2
• Organism: Escherichia coli strain FWSEC0390
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_RRNO01000155 (12777..12868 [-], 92 nt)
• oriT length: 92 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 92 nt

>oriT_FWSEC0390|unnamed2
CGGGGTGTCGGGGCTAAGCCCTGACCAGGTGGTAATCGTATAGCCGTGCGTGCGCGGTTATACGATTACACATCCTGTCCCGTTTTTCAGGC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_C9166_RS26790_FWSEC0390|unnamed2

• ID: 1538
• GenBank: WP_000991402
• Name: Relaxase_C9166_RS26790_FWSEC0390|unnamed2
• UniProt ID: F2W465
• Length: 906 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 906 a.a.
• Molecular weight: 105076.81 Da
• Isoelectric Point: 8.1396

>WP_000991402.1 relaxase/mobilization nuclease domain-containing protein [Escherichia coli]
MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEIPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCLSLESAAEEMQKAADLAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELEIKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIVQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDRAREGVALSSFGPLWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRHFAERLPEMARNGELESCLDVHRTLATAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVIPGERFRGRNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
GLIQCRSLIEEMIADGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRTTNVDRCVVLCEPGGTPLFNNVAKLEARLQK
NGSVHFRDTRTGKNVCTDYGDRVVFYHHTDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM
VAWHNAAGITGNGHFTITRPDVDLHRQRSEQYYREYIRQQTRLSVSHDDNYTLRQEKTWEPPSPGM

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (62-315)

Protein structure

• AlphaFold DB: F2W465

Auxiliary protein

WP_136764583_FWSEC0390|unnamed2

• ID: 617
• GenBank: WP_136764583
• Name: WP_136764583_FWSEC0390|unnamed2
• UniProt ID: _
• Length: 110 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 110 a.a.
• Molecular weight: 12639.76 Da
• Isoelectric Point: 10.6096

>WP_136764583.1 plasmid mobilization protein MobA [Escherichia coli]
MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVSTLIRKTVLVKQINARIDEDFLKELMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITELANTLRRDLMGR

Protein domains

Predicted by InterproScan.

• Family of unknown function (DUF6290) (DUF6290) (19-66)

Protein structure

No available structure.

Host bacterium

• ID: 2307
• GenBank: NZ_RRNO01000155
• Plasmid name: FWSEC0390|unnamed2
• Incompatibility group: -
• Plasmid size: 16541 bp
• Coordinate of oriT [Strand]: 12777..12868 [-]
• Host baterium: Escherichia coli strain FWSEC0390

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -