Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101862 |
| Name | oriT_pCRE22_5  |
| Organism | Enterobacter hormaechei strain S22_CRE22 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JAHBNR010000008 (1132..1189 [+], 58 nt) |
| oriT length | 58 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 58 nt
>oriT_pCRE22_5
GGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCT
GGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1537 | GenBank | WP_247652451 |
| Name | Relaxase_KIN47_RS26105_pCRE22_5 |
UniProt ID | _ |
| Length | 498 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 498 a.a. Molecular weight: 54960.03 Da Isoelectric Point: 6.7956
>WP_247652451.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MMIVKFHPRGRGGGGGPVDYLLGKDRQRDGASVLQGKPDEVRELIDASPYAKKYTSGVLSFAEQDLPPGQ
REKLMASFERVLMPGLDKDQYSVLWVEHRDKGRLELNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTIV
NGRLGLHDPNAPENRRVLVSPSALPEAKQEAAQAITSGLLALASSGELKTRQDVTEALESAGFEVVRTTK
SSISIADPDGGRNIRLKGAIYEQSFNAGEGLRAEIESAAADYRRDAESRIQRAREVCQSGTERKREENQR
RHPRPRPEAVLSHEPAHERDAAHGQPDVADHRSGLRAADSVERGHSVVAGAADTRELYDHPGAEEHAGHA
VREEQRRTALDLRRAETPLREGEPGSGTVRRGLELDDTGGEIAHDGAGKTVAERIRAATAGLLEKAGRVG
ERLRGMADDVWSYSTGERGAERARHGLEQAGAEFERAAAPVVERLNAIETHRQQERAVQHQKALELERSQ
WQHHGPSL
MMIVKFHPRGRGGGGGPVDYLLGKDRQRDGASVLQGKPDEVRELIDASPYAKKYTSGVLSFAEQDLPPGQ
REKLMASFERVLMPGLDKDQYSVLWVEHRDKGRLELNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTIV
NGRLGLHDPNAPENRRVLVSPSALPEAKQEAAQAITSGLLALASSGELKTRQDVTEALESAGFEVVRTTK
SSISIADPDGGRNIRLKGAIYEQSFNAGEGLRAEIESAAADYRRDAESRIQRAREVCQSGTERKREENQR
RHPRPRPEAVLSHEPAHERDAAHGQPDVADHRSGLRAADSVERGHSVVAGAADTRELYDHPGAEEHAGHA
VREEQRRTALDLRRAETPLREGEPGSGTVRRGLELDDTGGEIAHDGAGKTVAERIRAATAGLLEKAGRVG
ERLRGMADDVWSYSTGERGAERARHGLEQAGAEFERAAAPVVERLNAIETHRQQERAVQHQKALELERSQ
WQHHGPSL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 616 | GenBank | WP_004193914 |
| Name | WP_004193914_pCRE22_5 |
UniProt ID | A0A8T3UXZ2 |
| Length | 107 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 107 a.a. Molecular weight: 11783.55 Da Isoelectric Point: 7.8963
>WP_004193914.1 MULTISPECIES: MobC family plasmid mobilization relaxosome protein [Bacteria]
MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSISPALLRQLAGMGNNLNQIARQVNAG
GGSGHDRVQIVAALMAIDAGLERLRHAVLEKGADDDR
MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSISPALLRQLAGMGNNLNQIARQVNAG
GGSGHDRVQIVAALMAIDAGLERLRHAVLEKGADDDR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Host bacterium
| ID | 2306 | GenBank | NZ_JAHBNR010000008 |
| Plasmid name | pCRE22_5 | Incompatibility group | Col440II |
| Plasmid size | 3223 bp | Coordinate of oriT [Strand] | 1132..1189 [+] |
| Host baterium | Enterobacter hormaechei strain S22_CRE22 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |