Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101859
Name   oriT_pDCCK1-KPC in_silico
Organism   Enterobacter hormaechei subsp. hoffmannii strain HAC11Eho
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JAODVB010000019 (43701..43805 [-], 105 nt)
oriT length   105 nt
IRs (inverted repeats)      32..37, 40..45  (GCCGGC..GCCGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 105 nt

>oriT_pDCCK1-KPC
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1536 GenBank   WP_004206924
Name   Relaxase_N7U37_RS20855_pDCCK1-KPC insolico UniProt ID   A0A2V4FJ40
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75087.84 Da        Isoelectric Point: 10.0285

>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A2V4FJ40


T4CP

ID   1208 GenBank   WP_004206885
Name   t4cp2_N7U37_RS21110_pDCCK1-KPC insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79538.91 Da        Isoelectric Point: 4.8317

>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 21707..40903

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7U37_RS20725 (N7U37_20725) 17119..17307 - 189 Protein_16 MerC family mercury resistance protein -
N7U37_RS20730 (N7U37_20730) 17335..17610 - 276 WP_000732275 mercury resistance system periplasmic binding protein MerP -
N7U37_RS20735 (N7U37_20735) 17626..17976 - 351 WP_031623830 mercuric ion transporter MerT -
N7U37_RS20740 (N7U37_20740) 18048..18503 + 456 WP_001166628 Hg(II)-responsive transcriptional regulator -
N7U37_RS20745 (N7U37_20745) 19395..20450 - 1056 WP_230206039 plasmid replication initiator RepA -
N7U37_RS20750 (N7U37_20750) 20747..20977 - 231 WP_004187496 IncL/M type plasmid replication protein RepC -
N7U37_RS20755 (N7U37_20755) 21051..21704 - 654 WP_004206935 hypothetical protein -
N7U37_RS20760 (N7U37_20760) 21707..23887 - 2181 WP_004187492 DotA/TraY family protein traY
N7U37_RS20765 (N7U37_20765) 23880..24530 - 651 WP_004187488 hypothetical protein -
N7U37_RS20770 (N7U37_20770) 24527..25735 - 1209 WP_004187486 conjugal transfer protein TraW traW
N7U37_RS20775 (N7U37_20775) 25732..28782 - 3051 WP_011154474 conjugative transfer protein traU
N7U37_RS20780 (N7U37_20780) 28779..29273 - 495 WP_004187480 hypothetical protein -
N7U37_RS20785 (N7U37_20785) 29319..29708 - 390 WP_011154472 DUF6750 family protein traR
N7U37_RS20790 (N7U37_20790) 29725..30255 - 531 WP_004187478 conjugal transfer protein TraQ traQ
N7U37_RS20795 (N7U37_20795) 30279..30983 - 705 WP_004206933 conjugal transfer protein TraP traP
N7U37_RS20800 (N7U37_20800) 30995..32344 - 1350 WP_004206932 conjugal transfer protein TraO traO
N7U37_RS20805 (N7U37_20805) 32356..33507 - 1152 WP_015062843 DotH/IcmK family type IV secretion protein traN
N7U37_RS20810 (N7U37_20810) 33516..34229 - 714 WP_223294958 DotI/IcmL family type IV secretion protein traM
N7U37_RS20815 (N7U37_20815) 34297..34707 + 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
N7U37_RS20820 (N7U37_20820) 34736..34891 + 156 WP_172693602 Hha/YmoA family nucleoid-associated regulatory protein -
N7U37_RS20825 (N7U37_20825) 34892..35404 - 513 WP_011091071 hypothetical protein traL
N7U37_RS20830 (N7U37_20830) 35370..38675 - 3306 WP_242781107 LPD7 domain-containing protein -
N7U37_RS20835 (N7U37_20835) 38700..38960 - 261 WP_004187310 IcmT/TraK family protein traK
N7U37_RS20840 (N7U37_20840) 38950..40113 - 1164 WP_004206926 plasmid transfer ATPase TraJ virB11
N7U37_RS20845 (N7U37_20845) 40124..40903 - 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
N7U37_RS20850 (N7U37_20850) 40900..41400 - 501 WP_004206925 DotD/TraH family lipoprotein -
N7U37_RS20855 (N7U37_20855) 41414..43393 - 1980 WP_004206924 TraI/MobA(P) family conjugative relaxase -
N7U37_RS20860 (N7U37_20860) 43380..43697 - 318 WP_004206923 plasmid mobilization protein MobA -
N7U37_RS20865 (N7U37_20865) 43973..44338 + 366 WP_058672219 hypothetical protein -
N7U37_RS20870 (N7U37_20870) 44839..45375 - 537 WP_004206920 hypothetical protein -
N7U37_RS20875 (N7U37_20875) 45508..45819 - 312 WP_004187333 hypothetical protein -

Host bacterium

ID   2303 GenBank   NZ_JAODVB010000019
Plasmid name   pDCCK1-KPC Incompatibility group   IncL/M
Plasmid size   75775 bp Coordinate of oriT [Strand]   43701..43805 [-]
Host baterium   Enterobacter hormaechei subsp. hoffmannii strain HAC11Eho

Cargo genes

Drug resistance gene   blaKPC-2
Virulence gene   -
Metal resistance gene   merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -