Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101850 |
| Name | oriT_pTUECL19002-2  |
| Organism | Enterobacter hormaechei strain TUECL19002 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JAJTOV010000003 (42825..42930 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pTUECL19002-2
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1528 | GenBank | WP_004187323 |
| Name | Relaxase_LXO66_RS24070_pTUECL19002-2 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 1202 | GenBank | WP_004206885 |
| Name | t4cp2_LXO66_RS23765_pTUECL19002-2 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 45726..65015
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LXO66_RS24050 (LXO66_24050) | 40810..41121 | + | 312 | WP_004187333 | hypothetical protein | - |
| LXO66_RS24055 (LXO66_24055) | 41254..41790 | + | 537 | WP_004187332 | hypothetical protein | - |
| LXO66_RS24060 (LXO66_24060) | 42292..42687 | - | 396 | WP_019725163 | hypothetical protein | - |
| LXO66_RS24065 (LXO66_24065) | 42722..43249 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| LXO66_RS24070 (LXO66_24070) | 43236..45215 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| LXO66_RS24075 (LXO66_24075) | 45229..45729 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| LXO66_RS24080 (LXO66_24080) | 45726..46505 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| LXO66_RS24085 (LXO66_24085) | 46516..47679 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| LXO66_RS24090 (LXO66_24090) | 47669..47929 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| LXO66_RS24095 (LXO66_24095) | 47954..51391 | + | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
| LXO66_RS24100 (LXO66_24100) | 51357..51869 | + | 513 | WP_011091071 | hypothetical protein | traL |
| LXO66_RS24105 (LXO66_24105) | 51870..52082 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| LXO66_RS24110 (LXO66_24110) | 52054..52464 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| LXO66_RS24115 (LXO66_24115) | 52532..53245 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| LXO66_RS24120 (LXO66_24120) | 53254..54405 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| LXO66_RS24125 (LXO66_24125) | 54417..55766 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| LXO66_RS24130 (LXO66_24130) | 55778..56482 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| LXO66_RS24135 (LXO66_24135) | 56506..57036 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| LXO66_RS24140 (LXO66_24140) | 57053..57442 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| LXO66_RS24145 (LXO66_24145) | 57488..57982 | + | 495 | WP_004187480 | hypothetical protein | - |
| LXO66_RS24150 (LXO66_24150) | 57979..61029 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| LXO66_RS24155 (LXO66_24155) | 61026..62234 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| LXO66_RS24160 (LXO66_24160) | 62231..62827 | + | 597 | WP_015060003 | hypothetical protein | - |
| LXO66_RS24165 (LXO66_24165) | 62820..65015 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| LXO66_RS24170 (LXO66_24170) | 65017..65670 | + | 654 | WP_015060005 | hypothetical protein | - |
| LXO66_RS24175 (LXO66_24175) | 65751..65981 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 2294 | GenBank | NZ_JAJTOV010000003 |
| Plasmid name | pTUECL19002-2 | Incompatibility group | IncL/M |
| Plasmid size | 66276 bp | Coordinate of oriT [Strand] | 42825..42930 [+] |
| Host baterium | Enterobacter hormaechei strain TUECL19002 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |