Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 101844
• Name: oriT_pECC45-1
• Organism: Enterobacter hormaechei strain ECC45
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_JAIEUF010000002 (277433..277687 [+], 255 nt)
• oriT length: 255 nt
• IRs (inverted repeats): 153..158, 160..165 (AAAAGT..ACTTTT) 122..130, 135..143 (TTAAGGCTT..AAGCCTTAA) 49..55, 60..66 (GAATTTT..AAAATTC)
• Location of nic site: 78..79
• Conserved sequence flanking the nic site: TTTGGTTAAA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 255 nt

>oriT_pECC45-1
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobH_K2J56_RS25385_pECC45-1

• ID: 1524
• GenBank: WP_012477394
• Name: mobH_K2J56_RS25385_pECC45-1
• UniProt ID: A0A2Z2E7B6
• Length: 1050 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1050 a.a.
• Molecular weight: 118067.47 Da
• Isoelectric Point: 4.6347

>WP_012477394.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

Protein domains

Predicted by InterproScan.

• Putative helicase (TraI_2) (108-304)

Protein structure

• AlphaFold DB: A0A2Z2E7B6

T4CP

traD_K2J56_RS25380_pECC45-1

• ID: 1198
• GenBank: WP_001284073
• Name: traD_K2J56_RS25380_pECC45-1
• UniProt ID: _
• Length: 694 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 694 a.a.
• Molecular weight: 78123.91 Da
• Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (406-609)

• AAA-like domain (AAA_10) (199-249)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 116620..125827

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2J56_RS24505 (K2J56_24505)	111730..112446	-	717	WP_000838219	zinc finger-like domain-containing protein	-
K2J56_RS24510 (K2J56_24510)	112443..112643	-	201	WP_000594930	hypothetical protein	-
K2J56_RS24515 (K2J56_24515)	112663..113715	-	1053	WP_219978655	hypothetical protein	-
K2J56_RS24520 (K2J56_24520)	114192..115067	+	876	WP_000594611	RepB family plasmid replication initiator protein	-
K2J56_RS24525 (K2J56_24525)	116216..116569	+	354	WP_000423602	hypothetical protein	-
K2J56_RS24530 (K2J56_24530)	116587..116937	+	351	WP_001447770	type IV conjugative transfer system protein TraL	-
K2J56_RS24535 (K2J56_24535)	116949..117737	+	789	WP_000783153	TraE/TraK family type IV conjugative transfer system protein	traE
K2J56_RS24540 (K2J56_24540)	117737..119008	+	1272	WP_219978653	type-F conjugative transfer system secretin TraK	traK
K2J56_RS24545 (K2J56_24545)	119010..119471	+	462	WP_000521242	plasmid transfer protein HtdO	-
K2J56_RS24550 (K2J56_24550)	119461..120816	+	1356	WP_000351841	IncHI-type conjugal transfer protein TrhB	traB
K2J56_RS24555 (K2J56_24555)	120824..121306	+	483	WP_000377632	hypothetical protein	-
K2J56_RS24560 (K2J56_24560)	121425..122177	+	753	WP_182911723	protein-disulfide isomerase HtdT	-
K2J56_RS24565 (K2J56_24565)	122187..123137	+	951	WP_001022588	IncHI-type conjugal transfer lipoprotein TrhV	traV
K2J56_RS24570 (K2J56_24570)	123146..125827	+	2682	WP_000387412	TraC family protein	virb4
K2J56_RS24575 (K2J56_24575)	128376..128537	+	162	WP_001371949	hypothetical protein	-

Host bacterium

• ID: 2288
• GenBank: NZ_JAIEUF010000002
• Plasmid name: pECC45-1
• Incompatibility group: IncHI2
• Plasmid size: 294342 bp
• Coordinate of oriT [Strand]: 277433..277687 [+]
• Host baterium: Enterobacter hormaechei strain ECC45

Cargo genes

• Drug resistance gene: mcr-9, aph(6)-Id, aph(3'')-Ib, dfrA19, blaSHV-12, sul1, blaDHA-1, qnrB4, qacE, ere(A), aac(6')-IIc, catA2, tet(D)
• Virulence gene: -
• Metal resistance gene: rcnR/yohL, ncrC, pcoE, pcoS, terW, terZ, terA, terB, terC, terD, terE, pbrA, merE, merD, merA, merC, merP, merT, merR
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -