Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101802
Name   oriT_pEcl9-2 in_silico
Organism   Enterobacter hormaechei strain Eho-9
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JAAAXS010000004 (55910..55996 [-], 87 nt)
oriT length   87 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 87 nt

>oriT_pEcl9-2
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTGATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1510 GenBank   WP_022650008
Name   Relaxase_GVI70_RS23310_pEcl9-2 insolico UniProt ID   A0A4Q2QTK9
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 102314.37 Da        Isoelectric Point: 8.2756

>WP_022650008.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacterales]
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVRASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKTKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLAGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRGRNQSLREYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKE
SGELKVKDGWDSERPGVTLSAFGHAFDTDKLIRKLGEFVPPVQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYAIARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDSTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGSLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIESLIGSGEFTWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERVKPASRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRIRKARIRIEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGNNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMRFAEMTNQGSNTEAVRNESGPNWKPPSPL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(51-317)


  Protein structure


Source ID Structure
AlphaFold DB A0A4Q2QTK9


T4CP

ID   1188 GenBank   WP_022650006
Name   trbC_GVI70_RS23325_pEcl9-2 insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 87660.94 Da        Isoelectric Point: 6.8660

>WP_022650006.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MNYTPVNQALMKRNAWNSPVLELLRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRRFPSLFQYETTQESKGKGIFYLGYRRMNDIGRELWLTIDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IHNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAELYCRAIDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHRLE
GLDSEALEITKYKGPFPYMNYLDEVGAYYTERIAVQATQVRSLDFSIVMMSQDQERIESQTSATNVATLM
QNAGTKVAGKIVSDDKTAKTITNAAGQEARANMVSLQRRDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIEPQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAAKAEPWKIIDTFQQRLANRQEAHNLMTEYDTDHRGREENLWEEALEVIRTTTMAERTI
RYITLNKPESEPTQVSAEEIQLAPDAILRTLTLPQPAYIPPSRYRNEPFISPKLPDSPHPDMEWPE

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(440-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 62119..96563

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI70_RS23315 (GVI70_23260) 59135..59338 - 204 WP_123839396 hypothetical protein -
GVI70_RS23320 (GVI70_23265) 59472..59741 - 270 WP_022650007 hypothetical protein -
GVI70_RS23325 (GVI70_23270) 59815..62115 - 2301 WP_022650006 F-type conjugative transfer protein TrbC -
GVI70_RS23330 (GVI70_23275) 62119..63375 - 1257 WP_126530067 hypothetical protein trbB
GVI70_RS23335 (GVI70_23280) 63356..64564 - 1209 WP_022650004 hypothetical protein trbA
GVI70_RS23340 (GVI70_23285) 64885..65517 - 633 WP_022650003 plasmid IncI1-type surface exclusion protein ExcA -
GVI70_RS23345 (GVI70_23290) 65579..67738 - 2160 WP_022650002 DotA/TraY family protein traY
GVI70_RS23350 (GVI70_23295) 67796..68338 - 543 WP_022650001 conjugal transfer protein TraX -
GVI70_RS23355 (GVI70_23300) 68335..69537 - 1203 WP_022650000 conjugal transfer protein TraW traW
GVI70_RS23360 (GVI70_23305) 69550..70119 - 570 WP_022649999 hypothetical protein traV
GVI70_RS23365 (GVI70_23310) 70119..73172 - 3054 WP_022649998 hypothetical protein traU
GVI70_RS23370 (GVI70_23315) 73259..73984 - 726 WP_063864836 hypothetical protein traT
GVI70_RS23375 (GVI70_23320) 74168..74572 - 405 WP_022649996 DUF6750 family protein traR
GVI70_RS23380 (GVI70_23325) 74607..75131 - 525 WP_022649995 conjugal transfer protein TraQ traQ
GVI70_RS23385 (GVI70_23330) 75131..75904 - 774 WP_123839398 conjugal transfer protein TraP traP
GVI70_RS23390 (GVI70_23335) 75885..77192 - 1308 WP_022649993 conjugal transfer protein TraO traO
GVI70_RS23395 (GVI70_23340) 77192..78172 - 981 WP_022649992 DotH/IcmK family type IV secretion protein traN
GVI70_RS23400 (GVI70_23345) 78183..78800 - 618 WP_179297403 DotI/IcmL family type IV secretion protein traM
GVI70_RS23405 (GVI70_23350) 78890..79240 - 351 WP_022649990 hypothetical protein traL
GVI70_RS23410 (GVI70_23355) 79258..82800 - 3543 WP_032635082 LPD7 domain-containing protein -
GVI70_RS23415 (GVI70_23360) 82872..83159 - 288 WP_022649988 hypothetical protein traK
GVI70_RS23420 (GVI70_23365) 83156..84316 - 1161 WP_022649987 plasmid transfer ATPase TraJ virB11
GVI70_RS23425 (GVI70_23370) 84329..85135 - 807 WP_022649986 type IV secretory system conjugative DNA transfer family protein traI
GVI70_RS23430 (GVI70_23375) 85132..85605 - 474 WP_022649985 DotD/TraH family lipoprotein -
GVI70_RS23435 (GVI70_23380) 85676..86092 - 417 WP_123839394 hypothetical protein -
GVI70_RS23440 (GVI70_23385) 86201..87406 - 1206 WP_022649984 conjugal transfer protein TraF -
GVI70_RS23445 (GVI70_23390) 87512..88348 - 837 WP_022649983 hypothetical protein traE
GVI70_RS23450 (GVI70_23395) 88495..88971 - 477 WP_022649982 hypothetical protein -
GVI70_RS23455 (GVI70_23400) 88991..90151 - 1161 WP_022649981 site-specific integrase -
GVI70_RS23460 (GVI70_23405) 90729..92066 - 1338 WP_040110252 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
GVI70_RS23465 (GVI70_23410) 92063..92737 - 675 WP_022649979 prepilin peptidase -
GVI70_RS23470 (GVI70_23415) 92722..93204 - 483 WP_045261878 lytic transglycosylase domain-containing protein virB1
GVI70_RS23475 (GVI70_23420) 93289..93903 - 615 WP_022649977 type 4 pilus major pilin -
GVI70_RS23480 (GVI70_23425) 93917..95011 - 1095 WP_032623575 type II secretion system F family protein -
GVI70_RS23485 (GVI70_23430) 95004..96563 - 1560 WP_022649975 ATPase, T2SS/T4P/T4SS family virB11
GVI70_RS23490 (GVI70_23435) 96566..97081 - 516 WP_022649974 type IV pilus biogenesis protein PilP -
GVI70_RS23495 (GVI70_23440) 97065..98369 - 1305 WP_022649973 type 4b pilus protein PilO2 -
GVI70_RS23500 (GVI70_23445) 98371..100047 - 1677 WP_022649972 PilN family type IVB pilus formation outer membrane protein -
GVI70_RS23505 (GVI70_23450) 100062..100499 - 438 WP_022649971 type IV pilus biogenesis protein PilM -

Host bacterium

ID   2246 GenBank   NZ_JAAAXS010000004
Plasmid name   pEcl9-2 Incompatibility group   -
Plasmid size   106698 bp Coordinate of oriT [Strand]   55910..55996 [-]
Host baterium   Enterobacter hormaechei strain Eho-9

Cargo genes

Drug resistance gene   blaLAP-2, qnrS1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIC1, AcrIIA7