Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101797
Name   oriT_PECIMP|punnamed2 in_silico
Organism   Enterobacter hormaechei strain PECIMP
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_QHMI01000031 (33573..33663 [+], 91 nt)
oriT length   91 nt
IRs (inverted repeats)      35..42, 45..52  (AGTGCGAT..ATCGCACT)
 21..26, 40..45  (TAAATC..GATTTA)
Location of nic site      63..64
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 91 nt

>oriT_PECIMP|punnamed2
TATTTCATTTTTTTGTTTTTTAAATCAGTTAGTTAGTGCGATTTATCGCACTGCGTTAGGTGTATAGCAGGTTAAGGGCTAAAAAATAATC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1505 GenBank   WP_058672983
Name   mobF_DL189_RS22760_PECIMP|punnamed2 insolico UniProt ID   A0A9X7L0H4
Length   1072 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1072 a.a.        Molecular weight: 118901.56 Da        Isoelectric Point: 6.4635

>WP_058672983.1 MULTISPECIES: MobF family relaxase [Enterobacteriaceae]
MLDITTITRIDIASVVGYYADAKDDYYSKDSSFTAWHGQGAEALGLSGEVSSQRFKELLVGDIDPFTQMK
RSSGDATKERLGYDLTFSAPKGVSMQALIHGDKAIIAAHEKAVAAAVQEAEKLAQARTTHAGKTITQNTG
NLVVASFRHETSRALDPELHTHAFVMNMTQRGDGQWRALKNDELMRAKMHLGDVYKQELALELTKAGYEL
RYNNKNNTFDMAHFTDEQIRGFSRRSAQIEEGLAAMGLTRETADAQTKSRVSMATRDKKTDDYSREDIHK
EWVNRANELGIDFNDLSWAGKGKDGPQTSMNTVPNFTSPEVKADRAIQFALKSLSERDASFDQSRLLAVA
NKKAIGQASKQDVEAAYYRAVAKGAIIEGEARYTTTTDQRSKDAPPLPAMTKAEWQAQLTDKKGMNDSQA
KALVAEGIKSGRLKKTSHRVTTVEGIRVEKAVLNIEARGRGKVIAALTSEQVTVQLVGKTLKAEQQKSVV
DICTTTDRFVAAHGFAGTGKSYMTMSAKAVLESQGYNVTALAPYGTQKKSLEDEGMPARTVAAFVKAKDK
KIDEKSVVFIDEAGVIPARQMKVLMETIEKAGARAVFLGDTSQTKAVEAGKPFEQLISAGMQTSYMKDIQ
RQKNDVLLEAVKLAAEGHISGSLARLSNISAEKDQDKRLEAVANRWLSFSPEQREGTLIISGTNESRVIL
NSQIREALQLQGKGVEVNFLERVDSTQAERRDSKYYQVGQIIIPEKDYKNGLQRGESYRVLDTGPGNLLT
VAGSDGQKISFSPRTHKNLSVYTSVAAELAVGDKVMVTRNDKTLDVANGDRFTVTGTTEKGTFTLTNSKG
REIELDQKQAAYLSYAYATTVHKAQGLTCDRVLFNIDTRSLTTSKDVFYVGISRARHEVEIFTDDKKRLL
SSASRNSPKTTASEIDRFLGMEERYKDVNRSYGHSDGQQEKSYGHDAGHQTEEKENTMKNATEAAHDGPD
TSGTENTSQDLRQREQEEVNAAIRYEQRQEATPEMEANPYHDYERYADEGPDYGDMQDYSTYEEYERARD
VELPQHEHENHQHDHDKEGYGL

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(16-288)

AAA domain (AAA_30)

(480-656)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(855-899)


  Protein structure



No available structure.




T4CP

ID   1185 GenBank   WP_040223430
Name   t4cp2_DL189_RS22765_PECIMP|punnamed2 insolico UniProt ID   _
Length   510 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 510 a.a.        Molecular weight: 57917.09 Da        Isoelectric Point: 9.6871

>WP_040223430.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MTEQERGLIFLCSLLGPIPFVWFITAKFTYGINSETAHYLVPYLLKNTFNLWPLWAAIIAGVVIGLCGFI
GFIIYDKGRVYKGPRFKRILRGTKLARASTLAEKTKERGKKQLMIAGVPVPTEYETLHFSIGGATGTGKS
TVIKEAIYSSLKRGTDKRIVLDPNGEYVETFYRPGDVILSPYDKRSPGWSMFNEIRDDFDYDLYAVSIIQ
ESKERSAEEWYRYGRLIYREVSKKLLTLKRNPTMEEVMHWCTIVENDKLKEFVKGTPAEALFIKGAERAT
GSARFVLGDKLAPHLKMPAGDFSLRDWLEDDKPGTLFITWQEQMKKSLNPLISCWLDIMFSSLLGMGKRD
QRTLFFIDELESLEYLPNLQDLLTKGRKSGASVWAGYQTYSQLIERYGINIAETLLGSMRSGIVMGSSRL
GKETLEQMSRALGEIEGEVEHKQGNPFQAGIGNRPRIETRTVRAVTPTEISELKNLTGYVYFPGDLPVGK
FKTKYVKYTRKNPVPGIIMR

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-492)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 15493..33002

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DL189_RS22620 (DL189_22615) 10936..11700 + 765 WP_001389365 IS6-like element IS6100 family transposase -
DL189_RS25735 11840..11905 - 66 Protein_13 EAL domain-containing protein -
DL189_RS22635 (DL189_22630) 12110..12442 + 333 WP_045723726 hypothetical protein -
DL189_RS22640 (DL189_22635) 12636..12995 + 360 WP_058672990 hypothetical protein -
DL189_RS22645 (DL189_22640) 13000..13608 + 609 WP_058672989 recombinase family protein -
DL189_RS22650 (DL189_22645) 13605..13790 - 186 WP_024562121 ribbon-helix-helix domain-containing protein -
DL189_RS22655 (DL189_22650) 14075..14383 - 309 WP_000733764 hypothetical protein -
DL189_RS22660 (DL189_22655) 14387..14725 - 339 WP_000040982 hypothetical protein -
DL189_RS22665 (DL189_22660) 14722..15072 - 351 WP_058672988 hypothetical protein -
DL189_RS22670 (DL189_22665) 15076..15393 - 318 WP_058672987 H-NS family nucleoid-associated regulatory protein -
DL189_RS22675 (DL189_22670) 15493..16275 + 783 WP_058672986 lytic transglycosylase domain-containing protein virB1
DL189_RS22685 (DL189_22680) 16522..16818 + 297 WP_015060075 TrbC/VirB2 family protein virB2
DL189_RS22690 (DL189_22685) 16858..17175 + 318 WP_040223409 VirB3 family type IV secretion system protein virB3
DL189_RS22695 (DL189_22690) 17176..19749 + 2574 WP_040223410 VirB4 family type IV secretion/conjugal transfer ATPase virb4
DL189_RS22700 (DL189_22695) 19765..20475 + 711 WP_040223411 type IV secretion system protein virB5
DL189_RS22705 (DL189_22700) 20485..20727 + 243 WP_040223412 EexN family lipoprotein -
DL189_RS22710 (DL189_22705) 20744..21781 + 1038 WP_058672985 type IV secretion system protein virB6
DL189_RS26240 (DL189_22710) 21858..21989 + 132 WP_032493177 hypothetical protein -
DL189_RS22720 (DL189_22715) 22000..22695 + 696 WP_040223414 type IV secretion system protein virB8
DL189_RS22725 (DL189_22720) 22706..23593 + 888 WP_040223415 TrbG/VirB9 family P-type conjugative transfer protein virB9
DL189_RS22730 (DL189_22725) 23593..24726 + 1134 WP_040223416 type IV secretion system protein VirB10 virB10
DL189_RS22735 (DL189_22730) 24777..25778 + 1002 WP_058672984 ATPase, T2SS/T4P/T4SS family virB11
DL189_RS22740 (DL189_22735) 25835..26302 + 468 WP_050136493 phospholipase D family protein -
DL189_RS22745 (DL189_22740) 26299..26610 + 312 WP_040223418 hypothetical protein -
DL189_RS22750 (DL189_22745) 27230..27592 - 363 WP_024561906 hypothetical protein -
DL189_RS22755 (DL189_22750) 27585..28250 - 666 WP_040223419 DUF6710 family protein -
DL189_RS22760 (DL189_22755) 28247..31465 - 3219 WP_058672983 MobF family relaxase -
DL189_RS22765 (DL189_22760) 31470..33002 - 1533 WP_040223430 type IV secretion system DNA-binding domain-containing protein virb4
DL189_RS26060 33002..33427 - 426 WP_227660646 traK protein -
DL189_RS22775 (DL189_22770) 33895..34326 + 432 WP_040223429 plasmid stabilization protein StbA -
DL189_RS22780 (DL189_22775) 34335..35051 + 717 WP_040223428 StbB family protein -
DL189_RS22785 (DL189_22780) 35054..35422 + 369 WP_040223427 plasmid stabilization protein StbC -
DL189_RS26065 35486..35836 + 351 WP_227660645 hypothetical protein -
DL189_RS22795 (DL189_22790) 35895..36077 - 183 WP_040223426 hypothetical protein -
DL189_RS22800 (DL189_22795) 36152..36358 - 207 WP_048987680 DUF905 family protein -
DL189_RS25740 36539..37216 - 678 WP_050136486 DUF5417 domain-containing protein -

Host bacterium

ID   2241 GenBank   NZ_QHMI01000031
Plasmid name   PECIMP|punnamed2 Incompatibility group   IncN3
Plasmid size   40047 bp Coordinate of oriT [Strand]   33573..33663 [+]
Host baterium   Enterobacter hormaechei strain PECIMP

Cargo genes

Drug resistance gene   blaIMP-1, dfrB3, cmlA1, qacE, sul1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -