Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101769 |
| Name | oriT_pA8sk2_1  |
| Organism | Klebsiella pneumoniae strain CPE7 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JACYGQ010000002 (24731..24780 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | 33..34 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pA8sk2_1
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1487 | GenBank | WP_194849536 |
| Name | traI_IYY95_RS00360_pA8sk2_1 |
UniProt ID | _ |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190729.70 Da Isoelectric Point: 6.2403
>WP_194849536.1 conjugative transfer relaxase/helicase TraI [Klebsiella pneumoniae]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGGPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGQQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGSWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIRHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGGPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGQQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGSWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIRHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 1163 | GenBank | WP_048321702 |
| Name | traD_IYY95_RS00355_pA8sk2_1 |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 85940.04 Da Isoelectric Point: 5.0615
>WP_048321702.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Klebsiella]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAEEPSVRATEPSVLRLTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDEVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAEEPSVRATEPSVLRLTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDEVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 24173..52789
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IYY95_RS00150 | 19273..19623 | + | 351 | WP_064184873 | hypothetical protein | - |
| IYY95_RS00155 | 20255..20608 | + | 354 | WP_004152748 | hypothetical protein | - |
| IYY95_RS00160 | 20665..21012 | + | 348 | WP_004152749 | hypothetical protein | - |
| IYY95_RS00165 | 21107..21253 | + | 147 | WP_004152750 | hypothetical protein | - |
| IYY95_RS00170 | 21304..22137 | + | 834 | WP_004152751 | N-6 DNA methylase | - |
| IYY95_RS00175 | 22957..23778 | + | 822 | WP_004152492 | DUF932 domain-containing protein | - |
| IYY95_RS00180 | 23811..24140 | + | 330 | WP_011977736 | DUF5983 family protein | - |
| IYY95_RS00185 | 24173..24658 | - | 486 | WP_001568108 | transglycosylase SLT domain-containing protein | virB1 |
| IYY95_RS00190 | 25093..25485 | + | 393 | WP_032436755 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| IYY95_RS00195 | 25692..26387 | + | 696 | WP_023307551 | transcriptional regulator TraJ family protein | - |
| IYY95_RS00200 | 26471..26842 | + | 372 | WP_004208838 | TraY domain-containing protein | - |
| IYY95_RS00205 | 26896..27264 | + | 369 | WP_004178060 | type IV conjugative transfer system pilin TraA | - |
| IYY95_RS00210 | 27278..27583 | + | 306 | WP_004178059 | type IV conjugative transfer system protein TraL | traL |
| IYY95_RS00215 | 27603..28169 | + | 567 | WP_004152602 | type IV conjugative transfer system protein TraE | traE |
| IYY95_RS00220 | 28156..28896 | + | 741 | WP_004152497 | type-F conjugative transfer system secretin TraK | traK |
| IYY95_RS00225 | 28896..30320 | + | 1425 | WP_023307549 | F-type conjugal transfer pilus assembly protein TraB | traB |
| IYY95_RS00230 | 30313..30732 | + | 420 | Protein_44 | conjugal transfer pilus-stabilizing protein TraP | - |
| IYY95_RS00235 | 30944..31513 | + | 570 | WP_023307547 | type IV conjugative transfer system lipoprotein TraV | traV |
| IYY95_RS00240 | 31645..32055 | + | 411 | WP_023307546 | hypothetical protein | - |
| IYY95_RS00245 | 32060..32350 | + | 291 | WP_032423381 | hypothetical protein | - |
| IYY95_RS00250 | 32374..32592 | + | 219 | WP_004171484 | hypothetical protein | - |
| IYY95_RS00255 | 32593..32910 | + | 318 | WP_023307544 | hypothetical protein | - |
| IYY95_RS00260 | 32977..33381 | + | 405 | WP_023307543 | hypothetical protein | - |
| IYY95_RS00265 | 33378..33668 | + | 291 | WP_023307542 | hypothetical protein | - |
| IYY95_RS00270 | 33676..34074 | + | 399 | WP_072158599 | hypothetical protein | - |
| IYY95_RS00275 | 34146..36785 | + | 2640 | WP_048321693 | type IV secretion system protein TraC | virb4 |
| IYY95_RS00280 | 36785..37174 | + | 390 | WP_032436749 | type-F conjugative transfer system protein TrbI | - |
| IYY95_RS00285 | 37174..37800 | + | 627 | WP_023307538 | type-F conjugative transfer system protein TraW | traW |
| IYY95_RS00290 | 37844..38803 | + | 960 | WP_015065634 | conjugal transfer pilus assembly protein TraU | traU |
| IYY95_RS00295 | 38816..39454 | + | 639 | WP_015065635 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| IYY95_RS00300 | 39513..41468 | + | 1956 | WP_044061268 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| IYY95_RS00305 | 41500..41736 | + | 237 | WP_042922352 | conjugal transfer protein TrbE | - |
| IYY95_RS29645 | 41733..41918 | + | 186 | WP_032437912 | hypothetical protein | - |
| IYY95_RS00310 | 41964..42290 | + | 327 | WP_004152685 | hypothetical protein | - |
| IYY95_RS00315 | 42311..43063 | + | 753 | WP_015065637 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| IYY95_RS00320 | 43074..43313 | + | 240 | WP_004152687 | type-F conjugative transfer system pilin chaperone TraQ | - |
| IYY95_RS00325 | 43285..43842 | + | 558 | WP_032437911 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| IYY95_RS00330 | 43887..44329 | + | 443 | Protein_65 | F-type conjugal transfer protein TrbF | - |
| IYY95_RS00335 | 44307..45686 | + | 1380 | WP_011977731 | conjugal transfer pilus assembly protein TraH | traH |
| IYY95_RS00340 | 45686..48508 | + | 2823 | WP_049087265 | conjugal transfer mating-pair stabilization protein TraG | traG |
| IYY95_RS00345 | 48532..49134 | + | 603 | WP_023280878 | hypothetical protein | - |
| IYY95_RS00350 | 49386..50117 | + | 732 | WP_032415736 | conjugal transfer complement resistance protein TraT | - |
| IYY95_RS00355 | 50477..52789 | + | 2313 | WP_048321702 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
| ID | 2213 | GenBank | NZ_JACYGQ010000002 |
| Plasmid name | pA8sk2_1 | Incompatibility group | IncFII |
| Plasmid size | 272695 bp | Coordinate of oriT [Strand] | 24731..24780 [-] |
| Host baterium | Klebsiella pneumoniae strain CPE7 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | fecE, fecD, pcoE, pcoS, pcoR, pcoD, pcoC, pcoB, pcoA, silP, silA, silB, silF, silC, silR, silS, silE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |