Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101768 |
| Name | oriT_p2019n9506513_2  |
| Organism | Escherichia coli strain COL6 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JACYGE010000010 (55453..55505 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_p2019n9506513_2
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 1162 | GenBank | WP_015059539 |
| Name | t4cp2_IYX24_RS24960_p2019n9506513_2 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 720..24177
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IYX24_RS24915 | 81..716 | - | 636 | WP_000934977 | A24 family peptidase | - |
| IYX24_RS24920 | 720..1202 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| IYX24_RS24925 | 1268..1825 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| IYX24_RS24930 | 1870..2979 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| IYX24_RS24935 | 2970..4508 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| IYX24_RS24940 | 4533..5027 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| IYX24_RS24945 | 5011..6321 | - | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| IYX24_RS24950 | 6372..8015 | - | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| IYX24_RS24955 | 8008..8544 | - | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| IYX24_RS24960 | 8591..10549 | - | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| IYX24_RS24965 | 10565..11620 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| IYX24_RS24970 | 11639..12778 | - | 1140 | WP_000790640 | TrbI/VirB10 family protein | virB10 |
| IYX24_RS24975 | 12768..13469 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| IYX24_RS24980 | 13535..14269 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| IYX24_RS24985 | 14435..16792 | - | 2358 | WP_000548955 | VirB4 family type IV secretion system protein | virb4 |
| IYX24_RS24990 | 16798..17118 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| IYX24_RS25860 | 17189..17479 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| IYX24_RS25000 | 17479..18063 | - | 585 | WP_001177113 | lytic transglycosylase domain-containing protein | virB1 |
| IYX24_RS25005 | 18084..18482 | - | 399 | WP_001153665 | hypothetical protein | - |
| IYX24_RS25010 | 18601..19038 | - | 438 | WP_000539665 | type IV pilus biogenesis protein PilM | - |
| IYX24_RS25015 | 19044..20279 | - | 1236 | WP_015059538 | TcpQ domain-containing protein | - |
| IYX24_RS25020 | 20282..20581 | - | 300 | WP_000835764 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| IYX24_RS25025 | 20649..20930 | - | 282 | WP_000638823 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| IYX24_RS25030 | 20920..21171 | - | 252 | WP_000121741 | hypothetical protein | - |
| IYX24_RS25035 | 21271..21906 | - | 636 | WP_015059536 | hypothetical protein | - |
| IYX24_RS25040 | 21979..22266 | - | 288 | WP_001032611 | EexN family lipoprotein | - |
| IYX24_RS25045 | 22279..22533 | - | 255 | WP_001043555 | EexN family lipoprotein | - |
| IYX24_RS25050 | 22535..23176 | - | 642 | WP_001425343 | type IV secretion system protein | - |
| IYX24_RS25055 | 23182..24177 | - | 996 | WP_001028543 | type IV secretion system protein | virB6 |
| IYX24_RS25060 | 24181..24438 | - | 258 | WP_000739144 | hypothetical protein | - |
| IYX24_RS25065 | 24435..24737 | - | 303 | WP_001360345 | hypothetical protein | - |
| IYX24_RS25070 | 24718..24975 | - | 258 | WP_001542015 | hypothetical protein | - |
| IYX24_RS25075 | 25008..25454 | - | 447 | WP_001243165 | hypothetical protein | - |
| IYX24_RS25080 | 25465..25635 | - | 171 | WP_000550720 | hypothetical protein | - |
| IYX24_RS25085 | 25639..26082 | - | 444 | WP_000964330 | NfeD family protein | - |
| IYX24_RS25090 | 26467..27420 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| IYX24_RS25095 | 27447..27623 | - | 177 | WP_000753050 | hypothetical protein | - |
| IYX24_RS25100 | 27616..27831 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| IYX24_RS25105 | 27824..28276 | - | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 2212 | GenBank | NZ_JACYGE010000010 |
| Plasmid name | p2019n9506513_2 | Incompatibility group | IncI2 |
| Plasmid size | 70278 bp | Coordinate of oriT [Strand] | 55453..55505 [-] |
| Host baterium | Escherichia coli strain COL6 |
Cargo genes
| Drug resistance gene | ant(3'')-Ia, lnu(G), blaTEM-1B |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |