Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 101767 |
Name | oriT_p2019n9506513_1 |
Organism | Escherichia coli strain COL6 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_JACYGE010000002 (2698..3049 [+], 352 nt) |
oriT length | 352 nt |
IRs (inverted repeats) | 192..198, 206..212 (TATAAAA..TTTTATA) 104..110, 118..124 (TTTAAAT..ATTTAAA) 106..111, 113..118 (TAAATC..GATTTA) 93..98, 106..111 (GATTTA..TAAATC) 40..47, 50..57 (GCAAAAAC..GTTTTTGC) 4..11, 16..23 (TTGGTGGT..ACCACCAA) |
Location of nic site | _ |
Conserved sequence flanking the nic site |
_ |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 352 nt
AGGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTATCTTTAATATCATTGAGTTGTATTTGTGGATTTATATTGTTTAAATCTGATTTATTTAAAGCAGCGTCGTTAACGCAGCTACAGCAACGCGCCGACACCGCTTTGTAGGGGTGGTACTGACTATTTTTATAAAAAACATTATTTTATATTAGGGGTGCTGCTAGCGGCGCGGTGTGTTTTTTTATAGGATACCGTCAGGGGCGCTGCTAGCGGTGCGTCCCTGTTTGCACTATGAATTCTAGTGTTTCGAAATTAACTTTGTTTTATGTTTAAAAAAGGTAATATCTA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 1486 | GenBank | WP_194846234 |
Name | traI_IYX24_RS00280_p2019n9506513_1 | UniProt ID | _ |
Length | 1756 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 1756 a.a. Molecular weight: 192129.69 Da Isoelectric Point: 5.8357
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQYNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DTVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDVQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDVMTVVVPGRAEPASLPVSDSPFTALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDKATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISHQKSALNTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTSFADLGREINA
QIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETPDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEA
QQEAIQKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLEDAEGNTRLISPREAVAEGVT
LYTPDTIRVGSGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDDRQGWTDAINNAVQKGTAHDVL
EPKADREVMNAERLFSSARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGRTEQAVREIAGQERDRAAITEREAALPESVLRESQREREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
ID | 595 | GenBank | WP_001151530 |
Name | WP_001151530_p2019n9506513_1 | UniProt ID | A0A385JKG6 |
Length | 127 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 127 a.a. Molecular weight: 14525.54 Da Isoelectric Point: 4.8662
MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLELGLRVYEAQMERKESAFNQAEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSVEMERFFPKNDDE
Protein domains
Predicted by InterproScan.
Protein structure
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A385JKG6 |
T4CP
ID | 1160 | GenBank | WP_112013997 |
Name | traC_IYX24_RS00170_p2019n9506513_1 | UniProt ID | _ |
Length | 875 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 875 a.a. Molecular weight: 99299.24 Da Isoelectric Point: 6.5173
MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAA
TQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVKASWLAKKNQARIDDVVNFLKNASDSEQYAESPTIRSRLDEMIVLLDQYT
ANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRTLKKLNVIDEGWR
LLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLFPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
ID | 1161 | GenBank | WP_194846233 |
Name | traD_IYX24_RS00275_p2019n9506513_1 | UniProt ID | _ |
Length | 738 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 738 a.a. Molecular weight: 83851.54 Da Isoelectric Point: 5.1676
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFINGCIYWWCTTLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHEKYMIWCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEGVTQAEQPQQPQQPQQPQQ
PQQPQQPQQPQQPQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYE
AWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2127..30148
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
IYX24_RS25505 | 129..302 | - | 174 | Protein_1 | hypothetical protein | - |
IYX24_RS25510 | 372..578 | + | 207 | WP_000547968 | hypothetical protein | - |
IYX24_RS00095 | 603..890 | + | 288 | WP_000107535 | hypothetical protein | - |
IYX24_RS00100 | 1009..1830 | + | 822 | WP_001234469 | DUF932 domain-containing protein | - |
IYX24_RS00105 | 2127..2774 | - | 648 | WP_194846231 | transglycosylase SLT domain-containing protein | virB1 |
IYX24_RS00110 | 3050..3433 | + | 384 | WP_001151530 | conjugal transfer relaxosome DNA-binding protein TraM | - |
IYX24_RS00115 | 3625..4272 | + | 648 | WP_000338012 | transcriptional regulator TraJ family protein | - |
IYX24_RS00120 | 4392..4619 | + | 228 | WP_000589558 | conjugal transfer relaxosome protein TraY | - |
IYX24_RS00125 | 4653..5018 | + | 366 | WP_000340274 | type IV conjugative transfer system pilin TraA | - |
IYX24_RS00130 | 5032..5343 | + | 312 | WP_000012106 | type IV conjugative transfer system protein TraL | traL |
IYX24_RS00135 | 5365..5931 | + | 567 | WP_000399794 | type IV conjugative transfer system protein TraE | traE |
IYX24_RS00140 | 5918..6646 | + | 729 | WP_001230783 | type-F conjugative transfer system secretin TraK | traK |
IYX24_RS00145 | 6646..8073 | + | 1428 | WP_100040466 | F-type conjugal transfer pilus assembly protein TraB | traB |
IYX24_RS00150 | 8063..8650 | + | 588 | WP_000002771 | conjugal transfer pilus-stabilizing protein TraP | - |
IYX24_RS00155 | 8637..8957 | + | 321 | WP_001057280 | conjugal transfer protein TrbD | - |
IYX24_RS00160 | 8954..9469 | + | 516 | WP_000724020 | type IV conjugative transfer system lipoprotein TraV | traV |
IYX24_RS00165 | 9604..9825 | + | 222 | WP_069040908 | conjugal transfer protein TraR | - |
IYX24_RS00170 | 9985..12612 | + | 2628 | WP_112013997 | type IV secretion system protein TraC | virb4 |
IYX24_RS00175 | 12609..12995 | + | 387 | WP_000214096 | type-F conjugative transfer system protein TrbI | - |
IYX24_RS00180 | 12992..13624 | + | 633 | WP_001203720 | type-F conjugative transfer system protein TraW | traW |
IYX24_RS00185 | 13621..14613 | + | 993 | WP_000830838 | conjugal transfer pilus assembly protein TraU | traU |
IYX24_RS00190 | 14640..14948 | + | 309 | WP_000412447 | hypothetical protein | - |
IYX24_RS00195 | 15011..15532 | + | 522 | WP_000505572 | hypothetical protein | - |
IYX24_RS00200 | 15559..16197 | + | 639 | WP_000087531 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
IYX24_RS00205 | 16194..16565 | + | 372 | WP_021534930 | hypothetical protein | - |
IYX24_RS00210 | 16590..17012 | + | 423 | WP_001229309 | HNH endonuclease signature motif containing protein | - |
IYX24_RS00215 | 17009..18859 | + | 1851 | WP_015059015 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
IYX24_RS00220 | 18886..19143 | + | 258 | WP_053913405 | conjugal transfer protein TrbE | - |
IYX24_RS00225 | 19136..19879 | + | 744 | WP_001030405 | type-F conjugative transfer system pilin assembly protein TraF | traF |
IYX24_RS00230 | 20040..20276 | + | 237 | WP_000270010 | type-F conjugative transfer system pilin chaperone TraQ | - |
IYX24_RS00235 | 20263..20808 | + | 546 | WP_000059817 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
IYX24_RS00240 | 20798..21112 | + | 315 | WP_001384255 | P-type conjugative transfer protein TrbJ | - |
IYX24_RS00245 | 21066..21458 | + | 393 | WP_000126338 | F-type conjugal transfer protein TrbF | - |
IYX24_RS00250 | 21445..22818 | + | 1374 | WP_000944325 | conjugal transfer pilus assembly protein TraH | traH |
IYX24_RS00255 | 22815..25631 | + | 2817 | WP_194846232 | conjugal transfer mating-pair stabilization protein TraG | traG |
IYX24_RS00260 | 25664..26185 | + | 522 | WP_089516325 | conjugal transfer entry exclusion protein TraS | - |
IYX24_RS00265 | 26210..26941 | + | 732 | WP_000850424 | conjugal transfer complement resistance protein TraT | - |
IYX24_RS00270 | 27144..27881 | + | 738 | WP_000199911 | hypothetical protein | - |
IYX24_RS00275 | 27932..30148 | + | 2217 | WP_194846233 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
ID | 2211 | GenBank | NZ_JACYGE010000002 |
Plasmid name | p2019n9506513_1 | Incompatibility group | - |
Plasmid size | 60515 bp | Coordinate of oriT [Strand] | 2698..3049 [+] |
Host baterium | Escherichia coli strain COL6 |
Cargo genes
Drug resistance gene | - |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |