Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   101767
Name   oriT_p2019n9506513_1 in_silico
Organism   Escherichia coli strain COL6
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JACYGE010000002 (2698..3049 [+], 352 nt)
oriT length   352 nt
IRs (inverted repeats)      192..198, 206..212  (TATAAAA..TTTTATA)
 104..110, 118..124  (TTTAAAT..ATTTAAA)
 106..111, 113..118  (TAAATC..GATTTA)
 93..98, 106..111  (GATTTA..TAAATC)
 40..47, 50..57  (GCAAAAAC..GTTTTTGC)
 4..11, 16..23  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 352 nt

>oriT_p2019n9506513_1
AGGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTATCTTTAATATCATTGAGTTGTATTTGTGGATTTATATTGTTTAAATCTGATTTATTTAAAGCAGCGTCGTTAACGCAGCTACAGCAACGCGCCGACACCGCTTTGTAGGGGTGGTACTGACTATTTTTATAAAAAACATTATTTTATATTAGGGGTGCTGCTAGCGGCGCGGTGTGTTTTTTTATAGGATACCGTCAGGGGCGCTGCTAGCGGTGCGTCCCTGTTTGCACTATGAATTCTAGTGTTTCGAAATTAACTTTGTTTTATGTTTAAAAAAGGTAATATCTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   1486 GenBank   WP_194846234
Name   traI_IYX24_RS00280_p2019n9506513_1 insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 192129.69 Da        Isoelectric Point: 5.8357

>WP_194846234.1 conjugative transfer relaxase/helicase TraI [Escherichia coli]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQYNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DTVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDVQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDVMTVVVPGRAEPASLPVSDSPFTALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDKATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISHQKSALNTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTSFADLGREINA
QIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETPDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEA
QQEAIQKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLEDAEGNTRLISPREAVAEGVT
LYTPDTIRVGSGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDDRQGWTDAINNAVQKGTAHDVL
EPKADREVMNAERLFSSARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGRTEQAVREIAGQERDRAAITEREAALPESVLRESQREREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

(1434-1557)

(10-283)

(969-1156)

(575-626)

(633-712)


  Protein structure



No available structure.




Auxiliary protein


ID   595 GenBank   WP_001151530
Name   WP_001151530_p2019n9506513_1 insolico UniProt ID   A0A385JKG6
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14525.54 Da        Isoelectric Point: 4.8662

>WP_001151530.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacteriaceae]
MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLELGLRVYEAQMERKESAFNQAEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSVEMERFFPKNDDE

  Protein domains


Predicted by InterproScan.

(1-126)


  Protein structure


Source ID Structure
AlphaFold DB A0A385JKG6


T4CP


ID   1160 GenBank   WP_112013997
Name   traC_IYX24_RS00170_p2019n9506513_1 insolico UniProt ID   _
Length   875 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 875 a.a.        Molecular weight: 99299.24 Da        Isoelectric Point: 6.5173

>WP_112013997.1 type IV secretion system protein TraC [Escherichia coli]
MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAA
TQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVKASWLAKKNQARIDDVVNFLKNASDSEQYAESPTIRSRLDEMIVLLDQYT
ANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRTLKKLNVIDEGWR
LLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLFPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

(290-446)

(38-276)

(467-770)

  Protein structure



No available structure.



ID   1161 GenBank   WP_194846233
Name   traD_IYX24_RS00275_p2019n9506513_1 insolico UniProt ID   _
Length   738 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 738 a.a.        Molecular weight: 83851.54 Da        Isoelectric Point: 5.1676

>WP_194846233.1 type IV conjugative transfer system coupling protein TraD [Escherichia coli]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFINGCIYWWCTTLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHEKYMIWCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEGVTQAEQPQQPQQPQQPQQ
PQQPQQPQQPQQPQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYE
AWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

(173-560)

(32-128)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 2127..30148

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IYX24_RS25505 129..302 - 174 Protein_1 hypothetical protein -
IYX24_RS25510 372..578 + 207 WP_000547968 hypothetical protein -
IYX24_RS00095 603..890 + 288 WP_000107535 hypothetical protein -
IYX24_RS00100 1009..1830 + 822 WP_001234469 DUF932 domain-containing protein -
IYX24_RS00105 2127..2774 - 648 WP_194846231 transglycosylase SLT domain-containing protein virB1
IYX24_RS00110 3050..3433 + 384 WP_001151530 conjugal transfer relaxosome DNA-binding protein TraM -
IYX24_RS00115 3625..4272 + 648 WP_000338012 transcriptional regulator TraJ family protein -
IYX24_RS00120 4392..4619 + 228 WP_000589558 conjugal transfer relaxosome protein TraY -
IYX24_RS00125 4653..5018 + 366 WP_000340274 type IV conjugative transfer system pilin TraA -
IYX24_RS00130 5032..5343 + 312 WP_000012106 type IV conjugative transfer system protein TraL traL
IYX24_RS00135 5365..5931 + 567 WP_000399794 type IV conjugative transfer system protein TraE traE
IYX24_RS00140 5918..6646 + 729 WP_001230783 type-F conjugative transfer system secretin TraK traK
IYX24_RS00145 6646..8073 + 1428 WP_100040466 F-type conjugal transfer pilus assembly protein TraB traB
IYX24_RS00150 8063..8650 + 588 WP_000002771 conjugal transfer pilus-stabilizing protein TraP -
IYX24_RS00155 8637..8957 + 321 WP_001057280 conjugal transfer protein TrbD -
IYX24_RS00160 8954..9469 + 516 WP_000724020 type IV conjugative transfer system lipoprotein TraV traV
IYX24_RS00165 9604..9825 + 222 WP_069040908 conjugal transfer protein TraR -
IYX24_RS00170 9985..12612 + 2628 WP_112013997 type IV secretion system protein TraC virb4
IYX24_RS00175 12609..12995 + 387 WP_000214096 type-F conjugative transfer system protein TrbI -
IYX24_RS00180 12992..13624 + 633 WP_001203720 type-F conjugative transfer system protein TraW traW
IYX24_RS00185 13621..14613 + 993 WP_000830838 conjugal transfer pilus assembly protein TraU traU
IYX24_RS00190 14640..14948 + 309 WP_000412447 hypothetical protein -
IYX24_RS00195 15011..15532 + 522 WP_000505572 hypothetical protein -
IYX24_RS00200 15559..16197 + 639 WP_000087531 type-F conjugative transfer system pilin assembly protein TrbC trbC
IYX24_RS00205 16194..16565 + 372 WP_021534930 hypothetical protein -
IYX24_RS00210 16590..17012 + 423 WP_001229309 HNH endonuclease signature motif containing protein -
IYX24_RS00215 17009..18859 + 1851 WP_015059015 type-F conjugative transfer system mating-pair stabilization protein TraN traN
IYX24_RS00220 18886..19143 + 258 WP_053913405 conjugal transfer protein TrbE -
IYX24_RS00225 19136..19879 + 744 WP_001030405 type-F conjugative transfer system pilin assembly protein TraF traF
IYX24_RS00230 20040..20276 + 237 WP_000270010 type-F conjugative transfer system pilin chaperone TraQ -
IYX24_RS00235 20263..20808 + 546 WP_000059817 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
IYX24_RS00240 20798..21112 + 315 WP_001384255 P-type conjugative transfer protein TrbJ -
IYX24_RS00245 21066..21458 + 393 WP_000126338 F-type conjugal transfer protein TrbF -
IYX24_RS00250 21445..22818 + 1374 WP_000944325 conjugal transfer pilus assembly protein TraH traH
IYX24_RS00255 22815..25631 + 2817 WP_194846232 conjugal transfer mating-pair stabilization protein TraG traG
IYX24_RS00260 25664..26185 + 522 WP_089516325 conjugal transfer entry exclusion protein TraS -
IYX24_RS00265 26210..26941 + 732 WP_000850424 conjugal transfer complement resistance protein TraT -
IYX24_RS00270 27144..27881 + 738 WP_000199911 hypothetical protein -
IYX24_RS00275 27932..30148 + 2217 WP_194846233 type IV conjugative transfer system coupling protein TraD virb4


Host bacterium


ID   2211 GenBank   NZ_JACYGE010000002
Plasmid name   p2019n9506513_1 Incompatibility group   -
Plasmid size   60515 bp Coordinate of oriT [Strand]   2698..3049 [+]
Host baterium   Escherichia coli strain COL6

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -