Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 101755
• Name: oriT_pRHBSTW-00074_13
• Organism: Enterobacter asburiae strain RHBSTW-00074
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_JABXRP010000013 (4062..4103 [+], 42 nt)
• oriT length: 42 nt
• IRs (inverted repeats): 7..13, 25..31 (CGTTTCT..AGAAACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 42 nt

>oriT_pRHBSTW-00074_13
GCGCACCGTTTCTGAACGAAGTGAAGAAACGTCTAAGTGCGC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobA_HV056_RS27720_pRHBSTW-00074_13

• ID: 1479
• GenBank: WP_061351404
• Name: mobA_HV056_RS27720_pRHBSTW-00074_13
• UniProt ID: A0A7W3DK51
• Length: 369 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 369 a.a.
• Molecular weight: 42680.23 Da
• Isoelectric Point: 6.4015

>WP_061351404.1 MULTISPECIES: mobilization protein MobA [Enterobacterales]
MASYHLSVKTGGKGSASPHADYISRDGKYAREKDSDLEHKESGNMPAWAAHKPSEFWKAADTSERANGCT
YREIEIALPRELKPEQRLELVRDFVQQEIGDRHAYQFAIHNPKAAIAGGEQPHAHIMFSERINDGIHRDP
EQYFKRANTKEPERGGAKKARFGETPTERKEYLVAQRERWADLQNKHLERYQHADRVDSRSLKAQGIDRE
PERHLGAGQVQRFDTEQLQSILARREAERQAQQSRDERDCVIDVTTSLREAIHERDDASVKPAQQPEPEV
AAGITFDFEKEPDKLNALVSDAMKDVQEEIDLQSLVNDAMAEFQGIHQEMERQRERERLAEKQRQQEKER
QRLAEQIRQKPDKGWSFSR

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (42-218)

Protein structure

• AlphaFold DB: A0A7W3DK51

Host bacterium

• ID: 2199
• GenBank: NZ_JABXRP010000013
• Plasmid name: pRHBSTW-00074_13
• Incompatibility group: ColE10
• Plasmid size: 9581 bp
• Coordinate of oriT [Strand]: 4062..4103 [+]
• Host baterium: Enterobacter asburiae strain RHBSTW-00074

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -