Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101726
Name   oriT_pRHBSTW-00904_5 in_silico
Organism   Klebsiella pneumoniae strain RHBSTW-00904
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JABXPA010000005 (11377..11466 [-], 90 nt)
oriT length   90 nt
IRs (inverted repeats)      34..41, 44..51  (AGTGCGAT..ATCGCACT)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 90 nt

>oriT_pRHBSTW-00904_5
TATTTCATTTTTTGTTTTTTTAATCAGTTAGTTAGTGCGATTCATCGCACTGCGTTAGGTGTATAGCAGGTTAAGGGCTAAAAAATAATC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1464 GenBank   WP_024561907
Name   mobF_HV321_RS28620_pRHBSTW-00904_5 insolico UniProt ID   H1ZMV6
Length   1072 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1072 a.a.        Molecular weight: 118896.59 Da        Isoelectric Point: 6.4636

>WP_024561907.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRIDIASVVGYYADAKDDYYSKDSSFTAWHGQGAEALGLSGEVSSQRFKELLVGEIDPFTQMK
RSSGDATKERLGYDLTFSAPKGVSMQALIHGDKAIIAAHEKAVAAAVQEAEKLAQARTTHAGKTITQNTG
NLVVASFRHETSRALDPELHTHAFVMNMTQRGDGQWRALKNDELMRAKMHLGDVYKQELALELTKAGYEL
RYNNKNNTFDMAHFTDEQIRGFSRRSAQIEEGLAAMGLTRETADAQTKSRVSMATRDKKTDDYSREDIHK
EWVNRANELGIDFNDLSWAGKGKDGPQTSMNTVPNFTSPEVKADRAIQFALKSLSERDASFDQSRLLAVA
NKKAIGQASKQDIEAAYYRAVAKGAIIEGEARYTTTTDQRSKDAPPLPAMTKAEWQAQLTDKKGMNNDQA
KALVAEGIKSGRLKKTSHRVTTVEGIRVEKAVLNIEARGRGKVIAALTSEQVTAQLVGKTLKAEQQKSVV
DICTTTDRFVAAHGFAGTGKSYMTMSAKAVLESQGFNVTALAPYGTQKKSLEDEGMPARTVAAFVKAKDK
KIDEKSVVFIDEAGVIPARQMKVLMETIEKAGARAVFLGDTSQTKAVEAGKPFEQLISAGMQTSYMKDIQ
RQKNDVLLEAVKLAAEGHISGSLARLSNISAEKDQDKRLEAVANRWLSFSPEQREGTLIISGTNESRVIL
NSQIREALQLQGKGVEVNFLERVDSTQAERRDSKYYQVGQIIIPEKDYKNGLQRGESYRVLDTGPGNLLT
VAGSDGQKISFSPRTHKNLSVYTSVAAELAVGDKVMVTRNDKTLDVANGDRFTVTGTTEKGTFTLTNAKG
REIELDQKQAAYLSYAYATTVHKAQGLTCDRVLFNIDTRSLTTSKDVFYVGISRARHEVEIFTDDKKRLL
SSASRNSPKTTASEIDRFLGMEERYKDVNRSYGHSDGQQEKSYGHDAGHQTEEKENTMKNATEAAHDGPD
TSGTENTSQDLRQREQEEVNAAIRYEQRQEATPEMEANPYHDYERYADEGPDYGDMQDYSTYEEYERARD
VELPQHEHENHQHDHDKEGYGL

  Protein domains


Predicted by InterproScan.

TrwC relaxase (TrwC)

(16-288)

AAA domain (AAA_30)

(480-656)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(855-899)


  Protein structure


Source ID Structure
AlphaFold DB H1ZMV6


T4CP

ID   1143 GenBank   WP_032493179
Name   t4cp2_HV321_RS28615_pRHBSTW-00904_5 insolico UniProt ID   _
Length   510 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 510 a.a.        Molecular weight: 57912.96 Da        Isoelectric Point: 9.5127

>WP_032493179.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MTEQERGLIFLCSLLGPIPFVWFITAKFVYGINSETAHYLVPYLLKNTFNLWPLWVAIIAGAVIGLCGFI
GFIIYDKGRVYKGPRFKRVLRGTKLVRASSLAEKTKERDHKQLTVAGVPVPTEYETLHFSIGGATGTGKS
TIIKEAIYSSLKRGTDKRIVLDPNGEYVETFYRPGDVILSPYDKRSPGWSMFNEIRDDFDYDLYAVSIIQ
ESKERSAEEWYRYGRLIYREVSKKLLTLKRSPTMEEVMHWCTIVENEKLKEFVKGTPAEALFIKGAERAT
GSARFVLGDKLAPHLKMPAGDFSLRDWLEDDKPGTLFITWQEQMKKSLNPLISCWLDIMFSSLLGMGKRD
QRTLFFIDELESLEYLPNLQDLLTKGRKSGASVWAGYQTYSQLIERYGINIAETLLGSMRSGIVMGSSRL
GKETLEQMSRALGEIEGEVEHKQGNPFQAGIGNRPRIETRTVRAVTPTEISELKNLTGYVYFPGDLPVGK
FKTKHVQYTRKNPVPGIIMR

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-492)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 47022..57298

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV321_RS28790 (HV321_28790) 44985..45989 + 1005 WP_000427620 IS110-like element IS4321 family transposase -
HV321_RS28795 (HV321_28795) 46498..47010 - 513 WP_029697418 phospholipase D family protein -
HV321_RS28800 (HV321_28800) 47022..48023 - 1002 WP_001076634 ATPase, T2SS/T4P/T4SS family virB11
HV321_RS28805 (HV321_28805) 48074..49207 - 1134 WP_000101920 type IV secretion system protein VirB10 virB10
HV321_RS28810 (HV321_28810) 49207..50094 - 888 WP_000758230 TrbG/VirB9 family P-type conjugative transfer protein virB9
HV321_RS28815 (HV321_28815) 50105..50800 - 696 WP_001208352 type IV secretion system protein virB8
HV321_RS30485 50811..50942 - 132 WP_032493177 hypothetical protein -
HV321_RS28820 (HV321_28820) 51019..52056 - 1038 WP_014014948 type IV secretion system protein virB6
HV321_RS28825 (HV321_28825) 52073..52315 - 243 WP_000858958 EexN family lipoprotein -
HV321_RS28830 (HV321_28830) 52325..53026 - 702 WP_014014949 type IV secretion system protein virB5
HV321_RS28835 (HV321_28835) 53042..55615 - 2574 WP_040115085 VirB4 family type IV secretion/conjugal transfer ATPase virb4
HV321_RS28840 (HV321_28840) 55616..55933 - 318 WP_000462629 VirB3 family type IV secretion system protein virB3
HV321_RS28845 (HV321_28845) 55973..56314 - 342 WP_001121032 TrbC/VirB2 family protein virB2
HV321_RS28850 (HV321_28850) 56516..57298 - 783 WP_024562119 lytic transglycosylase domain-containing protein virB1
HV321_RS28855 (HV321_28855) 57398..57715 + 318 WP_000960955 H-NS family nucleoid-associated regulatory protein -
HV321_RS28860 (HV321_28860) 57719..58069 + 351 WP_024562120 hypothetical protein -
HV321_RS28865 (HV321_28865) 58066..58404 + 339 WP_032493175 hypothetical protein -
HV321_RS28870 (HV321_28870) 58408..58716 + 309 WP_000733764 hypothetical protein -
HV321_RS28875 (HV321_28875) 59001..59186 + 186 WP_024562121 ribbon-helix-helix domain-containing protein -
HV321_RS28880 (HV321_28880) 59183..59791 - 609 WP_000275218 recombinase family protein -
HV321_RS28885 (HV321_28885) 59978..60310 - 333 WP_032493174 hypothetical protein -
HV321_RS28890 (HV321_28890) 60540..61666 + 1127 WP_085954994 IS3-like element ISSen4 family transposase -

Host bacterium

ID   2170 GenBank   NZ_JABXPA010000005
Plasmid name   pRHBSTW-00904_5 Incompatibility group   IncN3
Plasmid size   62709 bp Coordinate of oriT [Strand]   11377..11466 [-]
Host baterium   Klebsiella pneumoniae strain RHBSTW-00904

Cargo genes

Drug resistance gene   sul1, qacE, ant(3'')-Ia, blaOXA-10, cmlA1, ant(2'')-Ia
Virulence gene   -
Metal resistance gene   arsR, arsD, arsA, arsB, arsC, merT, merP, merC, merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -