Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101688 |
| Name | oriT_pCOL701T  |
| Organism | Escherichia coli strain COL701 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_VMKR01000024 (43468..43520 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pCOL701T
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 1131 | GenBank | WP_015059539 |
| Name | t4cp2_FPQ08_RS16530_pCOL701T |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73404.02 Da Isoelectric Point: 9.4339
>WP_015059539.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MNAKKMGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LDQKAKGHNAPDVLVSISAILKTSIPDNGKDLAAWMGQEVENRSWISDKTKSFFFEFMSAPDRTRGSIKT
NFSSPLNIFSNPVTAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1688..24518
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FPQ08_RS16480 (FPQ08_16570) | 1..1036 | - | 1036 | WP_000750512 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| FPQ08_RS16485 (FPQ08_16575) | 1049..1684 | - | 636 | WP_000934977 | A24 family peptidase | - |
| FPQ08_RS16490 (FPQ08_16580) | 1688..2170 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| FPQ08_RS16495 (FPQ08_16585) | 2236..2799 | - | 564 | WP_034169414 | type 4 pilus major pilin | - |
| FPQ08_RS16500 (FPQ08_16590) | 2849..3958 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| FPQ08_RS16505 (FPQ08_16595) | 3949..5487 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| FPQ08_RS16510 (FPQ08_16600) | 5512..6006 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| FPQ08_RS16515 (FPQ08_16605) | 5990..7300 | - | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| FPQ08_RS16520 (FPQ08_16610) | 7351..8994 | - | 1644 | WP_001035592 | PilN family type IVB pilus formation outer membrane protein | - |
| FPQ08_RS16525 (FPQ08_16615) | 8987..9523 | - | 537 | WP_001220543 | sigma 54-interacting transcriptional regulator | virb4 |
| FPQ08_RS16530 (FPQ08_16620) | 9570..11528 | - | 1959 | WP_015059539 | type IV secretory system conjugative DNA transfer family protein | - |
| FPQ08_RS16535 (FPQ08_16625) | 11544..12599 | - | 1056 | WP_001542006 | P-type DNA transfer ATPase VirB11 | virB11 |
| FPQ08_RS16540 (FPQ08_16630) | 12618..13757 | - | 1140 | WP_034169415 | TrbI/VirB10 family protein | virB10 |
| FPQ08_RS16545 (FPQ08_16635) | 13747..14448 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | - |
| FPQ08_RS16550 (FPQ08_16640) | 14514..15248 | - | 735 | WP_000432282 | type IV secretion system protein | virB8 |
| FPQ08_RS16555 (FPQ08_16650) | 15414..17771 | - | 2358 | WP_000548950 | VirB4 family type IV secretion system protein | virb4 |
| FPQ08_RS16560 (FPQ08_16655) | 17777..18097 | - | 321 | WP_000362080 | VirB3 family type IV secretion system protein | virB3 |
| FPQ08_RS22595 | 18168..18458 | - | 291 | WP_000865479 | conjugal transfer protein | - |
| FPQ08_RS16570 (FPQ08_16665) | 18458..19042 | - | 585 | WP_001177117 | lytic transglycosylase domain-containing protein | virB1 |
| FPQ08_RS16575 (FPQ08_16670) | 19063..19461 | - | 399 | WP_001153669 | hypothetical protein | - |
| FPQ08_RS16580 (FPQ08_16675) | 19580..20017 | - | 438 | WP_034169416 | type IV pilus biogenesis protein PilM | - |
| FPQ08_RS16585 (FPQ08_16680) | 20023..21258 | - | 1236 | WP_034169417 | toxin co-regulated pilus biosynthesis Q family protein | - |
| FPQ08_RS16590 (FPQ08_16685) | 21261..21560 | - | 300 | WP_000835763 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| FPQ08_RS16595 (FPQ08_16690) | 21608..22417 | + | 810 | WP_024237698 | DUF5710 domain-containing protein | - |
| FPQ08_RS16600 (FPQ08_16695) | 22640..22864 | - | 225 | WP_000713562 | EexN family lipoprotein | - |
| FPQ08_RS16605 (FPQ08_16700) | 22873..23517 | - | 645 | WP_001310442 | type IV secretion system protein | - |
| FPQ08_RS16610 (FPQ08_16705) | 23523..24518 | - | 996 | WP_001028540 | type IV secretion system protein | virB6 |
| FPQ08_RS16615 (FPQ08_16710) | 24522..24779 | - | 258 | WP_000739144 | hypothetical protein | - |
| FPQ08_RS16620 (FPQ08_16715) | 24776..25078 | - | 303 | WP_001360345 | hypothetical protein | - |
| FPQ08_RS22280 (FPQ08_16720) | 25059..25316 | - | 258 | WP_001542015 | hypothetical protein | - |
| FPQ08_RS16625 (FPQ08_16725) | 25349..25795 | - | 447 | WP_001243165 | hypothetical protein | - |
| FPQ08_RS22285 | 25806..25976 | - | 171 | WP_000550720 | hypothetical protein | - |
| FPQ08_RS16630 (FPQ08_16730) | 25980..26423 | - | 444 | WP_000964330 | NfeD family protein | - |
| FPQ08_RS16635 (FPQ08_16740) | 26797..27750 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| FPQ08_RS22290 | 27777..27953 | - | 177 | WP_000753050 | hypothetical protein | - |
| FPQ08_RS16640 (FPQ08_16745) | 27946..28161 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| FPQ08_RS16645 (FPQ08_16750) | 28154..28606 | - | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 2132 | GenBank | NZ_VMKR01000024 |
| Plasmid name | pCOL701T | Incompatibility group | IncI2 |
| Plasmid size | 59087 bp | Coordinate of oriT [Strand] | 43468..43520 [-] |
| Host baterium | Escherichia coli strain COL701 |
Cargo genes
| Drug resistance gene | mcr-1.1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |