Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101686
Name   oriT_pMER701T in_silico
Organism   Escherichia coli strain MER701
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_VMKT01000022 (12136..12208 [-], 73 nt)
oriT length   73 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 73 nt

>oriT_pMER701T
GTCGGGGCAAAGCCCTGACCAGGTGGGGAATGTCTGAGTGCGCGTGCGCGGTCCGACATTCCCACATCCTGTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1455 GenBank   WP_058100850
Name   Relaxase_FPQ09_RS16020_pMER701T insolico UniProt ID   A0A1M0DNA5
Length   906 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 906 a.a.        Molecular weight: 105098.51 Da        Isoelectric Point: 8.3663

>WP_058100850.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDVPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCNSLETAAEEMQYKADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRHFAERLPAMARNGELKSCLAVHRTLAEAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWHSVPKDIFTQVIPGERFRGHSIGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
ALIQCRSQIEEMIAGGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAAADIFERVKPECRYNPELAASDEVEPGFRRDPELRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAHIRVQFRDPQLRKLHYHIAEVQRMQALIRLKENVREERLALMAEGKWYP
LSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRTTNADRCVVLCEPGGTPLFNNVAKLEARLQK
NGSVHFRDTRTGKNVCTDYGDRVVFYHHTDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM
VAWHNAAGITGNGHFTITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWEPPSPGM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-315)


  Protein structure


Source ID Structure
AlphaFold DB A0A1M0DNA5


Auxiliary protein

ID   580 GenBank   WP_001291058
Name   WP_001291058_pMER701T insolico UniProt ID   A0A7I0L241
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12730.52 Da        Isoelectric Point: 9.9656

>WP_001291058.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVSTFIRSSSLDKVVNCRTDDRMIDEIMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITEYVNALRRDLMGR

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A7I0L241


T4CP

ID   1130 GenBank   WP_058100849
Name   trbC_FPQ09_RS16025_pMER701T insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 87195.08 Da        Isoelectric Point: 6.6676

>WP_058100849.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIWPLALPVCLLFTLVTSVIFTLQRWRCP
LRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQYEADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHII
FFSTTGGGKTETTFAWLLNPLCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEI
IQSGEKSRPQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQFSE
TFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSALGRMFITQQSMILARDLGYRLE
GTDAQTLEVKKYKGRFPYICILDEVGAYYTDRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLM
QNAGTKFAGRIVSDDKTARTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLN
AGEFFTVFQGDVVPSASVYIPDSEKSCDSDPVVINRYIAIEAPRLERLRRLVPRTVQRRLPTPEHVSSII
GVLTEKTSRKRRKKHTEPYRIIDTFQDQLKRTQTSLDLLPQYDTDIESRANELWKKAVHTINNTTREERR
VCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEILLPVAENNKSQTINNDHPQKSCGAQPTTDEKY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 17794..59410

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPQ09_RS16025 (FPQ09_16135) 15513..17813 - 2301 WP_058100849 F-type conjugative transfer protein TrbC -
FPQ09_RS16030 (FPQ09_16140) 17794..18918 - 1125 WP_058100848 DsbC family protein trbB
FPQ09_RS16035 (FPQ09_16145) 18915..20177 - 1263 WP_045145416 IncI1-type conjugal transfer protein TrbA trbA
FPQ09_RS16040 (FPQ09_16150) 20842..21309 - 468 WP_045145415 thermonuclease family protein -
FPQ09_RS16045 (FPQ09_16155) 21474..22097 + 624 WP_045145414 helix-turn-helix domain-containing protein -
FPQ09_RS16050 (FPQ09_16165) 22295..22510 - 216 WP_000266543 hypothetical protein -
FPQ09_RS16055 (FPQ09_16170) 22514..22882 - 369 WP_001302696 hypothetical protein -
FPQ09_RS16060 (FPQ09_16180) 23182..23424 - 243 WP_000650933 hypothetical protein -
FPQ09_RS16065 (FPQ09_16185) 23753..23905 + 153 WP_021532728 Hok/Gef family protein -
FPQ09_RS16070 (FPQ09_16195) 24474..24626 + 153 WP_001367784 Hok/Gef family protein -
FPQ09_RS22970 (FPQ09_16205) 24940..25071 - 132 Protein_31 ethanolamine utilization protein EutE -
FPQ09_RS16080 (FPQ09_16210) 25166..25375 + 210 WP_000062946 HEAT repeat domain-containing protein -
FPQ09_RS16085 (FPQ09_16215) 25440..26087 - 648 WP_072643575 plasmid IncI1-type surface exclusion protein ExcA -
FPQ09_RS16090 (FPQ09_16220) 26175..28334 - 2160 WP_058100846 DotA/TraY family protein traY
FPQ09_RS16095 (FPQ09_16225) 28407..28976 - 570 WP_058100845 conjugal transfer protein TraX -
FPQ09_RS16100 (FPQ09_16230) 28973..30178 - 1206 WP_052908626 conjugal transfer protein TraW traW
FPQ09_RS16105 (FPQ09_16235) 30139..30756 - 618 WP_052908627 hypothetical protein traV
FPQ09_RS16110 (FPQ09_16240) 30756..33800 - 3045 WP_058100844 conjugal transfer protein traU
FPQ09_RS16115 (FPQ09_16245) 33976..34356 - 381 WP_058100843 hypothetical protein -
FPQ09_RS16120 34356..34715 - 360 WP_053287752 hypothetical protein -
FPQ09_RS16125 (FPQ09_16250) 34783..35409 - 627 WP_077250482 conjugal transfer protein TraT traT
FPQ09_RS23225 (FPQ09_16255) 35516..35767 - 252 WP_001277575 hypothetical protein -
FPQ09_RS16135 (FPQ09_16260) 35824..36222 - 399 WP_029391936 DUF6750 family protein traR
FPQ09_RS16140 (FPQ09_16265) 36269..36799 - 531 WP_000987022 conjugal transfer protein TraQ traQ
FPQ09_RS16145 (FPQ09_16270) 36796..37509 - 714 WP_072645886 conjugal transfer protein TraP -
FPQ09_RS16150 (FPQ09_16275) 37506..38837 - 1332 WP_072645887 conjugal transfer protein TraO traO
FPQ09_RS16155 (FPQ09_16280) 38841..39815 - 975 WP_050923626 DotH/IcmK family type IV secretion protein traN
FPQ09_RS16160 (FPQ09_16285) 39826..40521 - 696 WP_021532714 DotI/IcmL family type IV secretion protein traM
FPQ09_RS16165 (FPQ09_16290) 40533..40883 - 351 WP_001536625 hypothetical protein traL
FPQ09_RS16170 (FPQ09_16295) 40900..44961 - 4062 WP_072645888 LPD7 domain-containing protein -
FPQ09_RS16175 (FPQ09_16300) 45025..45315 - 291 WP_000817799 hypothetical protein traK
FPQ09_RS16180 (FPQ09_16305) 45312..46460 - 1149 WP_058100838 plasmid transfer ATPase TraJ virB11
FPQ09_RS16185 (FPQ09_16310) 46444..47280 - 837 WP_000745049 type IV secretory system conjugative DNA transfer family protein traI
FPQ09_RS16190 (FPQ09_16315) 47277..47735 - 459 WP_001170111 DotD/TraH family lipoprotein -
FPQ09_RS16195 (FPQ09_16320) 47839..49041 - 1203 WP_053287757 conjugal transfer protein TraF -
FPQ09_RS16200 (FPQ09_16325) 49143..49964 - 822 WP_001681675 hypothetical protein traE
FPQ09_RS16205 (FPQ09_16330) 50238..50558 + 321 WP_000695052 hypothetical protein -
FPQ09_RS16210 (FPQ09_16335) 50650..50940 - 291 WP_021532709 hypothetical protein -
FPQ09_RS16215 (FPQ09_16340) 50982..52151 - 1170 WP_021532708 site-specific integrase -
FPQ09_RS16220 (FPQ09_16370) 53398..53637 + 240 WP_000271302 hypothetical protein -
FPQ09_RS16225 (FPQ09_16375) 53642..55015 - 1374 WP_053287758 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
FPQ09_RS16230 (FPQ09_16380) 55012..55719 - 708 WP_152920992 A24 family peptidase -
FPQ09_RS16235 (FPQ09_16385) 55679..56155 - 477 WP_029391914 lytic transglycosylase domain-containing protein virB1
FPQ09_RS16240 (FPQ09_16390) 56214..56750 - 537 WP_058100836 type 4 pilus major pilin -
FPQ09_RS16245 (FPQ09_16395) 56800..57900 - 1101 WP_001696153 type II secretion system F family protein -
FPQ09_RS16250 (FPQ09_16400) 57902..59410 - 1509 WP_021532701 ATPase, T2SS/T4P/T4SS family virB11
FPQ09_RS16255 (FPQ09_16405) 59507..60103 - 597 WP_029391917 hypothetical protein -
FPQ09_RS16260 (FPQ09_16410) 60199..60753 - 555 WP_072643479 hypothetical protein -
FPQ09_RS16265 (FPQ09_16415) 60802..61254 - 453 WP_021547657 type IV pilus biogenesis protein PilP -
FPQ09_RS16270 (FPQ09_16420) 61244..62539 - 1296 WP_077250483 type 4b pilus protein PilO2 -
FPQ09_RS16275 (FPQ09_16425) 62560..64179 - 1620 WP_021532696 PilN family type IVB pilus formation outer membrane protein -

Host bacterium

ID   2130 GenBank   NZ_VMKT01000022
Plasmid name   pMER701T Incompatibility group   IncB/O/K/Z
Plasmid size   75819 bp Coordinate of oriT [Strand]   12136..12208 [-]
Host baterium   Escherichia coli strain MER701

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -