Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101651
Name   oriT_FWSEC0544|unnamed4 in_silico
Organism   Escherichia coli strain FWSEC0544
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_RRSK01000247 (10555..10678 [-], 124 nt)
oriT length   124 nt
IRs (inverted repeats)      92..99, 113..120  (ATAATGTA..TACATTAT)
 90..95, 107..112  (AAATAA..TTATTT)
 39..46, 49..56  (GCAAAAAC..GTTTTTGC)
 3..10, 15..22  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 124 nt

>oriT_FWSEC0544|unnamed4
GGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGCTATTTGAATCATTAACTTATGTTTTAAATAATGTATTTTAATTTATTTTACATTATAAAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP

ID   1104 GenBank   WP_001064239
Name   traC_C9311_RS27465_FWSEC0544|unnamed4 insolico UniProt ID   _
Length   875 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 875 a.a.        Molecular weight: 99485.47 Da        Isoelectric Point: 6.6265

>WP_001064239.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacteriaceae]
MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAA
TQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKKKQARIDDVVDFLKNARDNDQYVESPTIRSRLDEMIVLLDQYT
ANGTYGRYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRTLKKLNVIDEGWR
LLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLFPDQFQPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGM
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(467-771)

Family of unknown function (DUF5934) (DUF5934)

(290-446)

TraC protein (TraC_F_IV)

(38-276)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 511..11250

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9311_RS30730 (C9311_27505) 1..116 - 116 Protein_0 conjugal transfer protein TraW -
C9311_RS27460 (C9311_27510) 113..514 - 402 WP_136754708 type-F conjugative transfer system protein TrbI -
C9311_RS27465 (C9311_27515) 511..3138 - 2628 WP_001064239 type IV secretion system protein TraC virb4
C9311_RS27470 (C9311_27520) 3298..3519 - 222 WP_001278978 conjugal transfer protein TraR -
C9311_RS27475 (C9311_27525) 3654..4169 - 516 WP_000809893 type IV conjugative transfer system lipoprotein TraV traV
C9311_RS27480 (C9311_27530) 4166..4417 - 252 WP_001038341 conjugal transfer protein TrbG -
C9311_RS27485 (C9311_27535) 4410..4730 - 321 WP_001057302 conjugal transfer protein TrbD virb4
C9311_RS27490 (C9311_27540) 4717..5301 - 585 WP_000002795 conjugal transfer pilus-stabilizing protein TraP -
C9311_RS27495 (C9311_27545) 5291..6718 - 1428 WP_000146638 F-type conjugal transfer pilus assembly protein TraB traB
C9311_RS27500 (C9311_27550) 6718..7446 - 729 WP_250206194 type-F conjugative transfer system secretin TraK traK
C9311_RS27505 (C9311_27555) 7433..7999 - 567 WP_000399804 type IV conjugative transfer system protein TraE traE
C9311_RS27510 (C9311_27560) 8021..8332 - 312 WP_000012106 type IV conjugative transfer system protein TraL traL
C9311_RS27515 (C9311_27565) 8337..8699 - 363 WP_001098998 type IV conjugative transfer system pilin TraA -
C9311_RS27520 (C9311_27570) 8733..8960 - 228 WP_001254388 conjugal transfer relaxosome protein TraY -
C9311_RS27525 (C9311_27575) 9054..9740 - 687 WP_000332487 PAS domain-containing protein -
C9311_RS27530 (C9311_27580) 9934..10317 - 384 WP_001151564 conjugal transfer relaxosome DNA-binding protein TraM -
C9311_RS27535 (C9311_27585) 10603..11250 + 648 WP_000614282 transglycosylase SLT domain-containing protein virB1
C9311_RS27540 (C9311_27590) 11547..12368 - 822 WP_001234469 DUF932 domain-containing protein -
C9311_RS27545 (C9311_27595) 12489..12776 - 288 WP_000107537 hypothetical protein -
C9311_RS30970 12801..12982 - 182 WP_250206195 single-stranded DNA-binding protein -

Host bacterium

ID   2095 GenBank   NZ_RRSK01000247
Plasmid name   FWSEC0544|unnamed4 Incompatibility group   -
Plasmid size   12982 bp Coordinate of oriT [Strand]   10555..10678 [-]
Host baterium   Escherichia coli strain FWSEC0544

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -