Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101648
Name   oriT_pE9211p1 in_silico
Organism   Escherichia coli O104:H4 str. E92/11
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AHAU01000146 (17706..17794 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pE9211p1
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1435 GenBank   WP_001387486
Name   nikB_E9211_RS00450_pE9211p1 insolico UniProt ID   D9Z503
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103861.32 Da        Isoelectric Point: 7.4288

>WP_001387486.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRNRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNRGGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB D9Z503


Auxiliary protein

ID   568 GenBank   WP_001283947
Name   WP_001283947_pE9211p1 insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   1103 GenBank   WP_001289278
Name   trbC_E9211_RS00445_pE9211p1 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86924.02 Da        Isoelectric Point: 6.7713

>WP_001289278.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILTAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKNNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 23172..53807

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E9211_RS00445 (E9211_49430) 20888..23179 - 2292 WP_001289278 F-type conjugative transfer protein TrbC -
E9211_RS00440 (E9211_49440) 23172..24242 - 1071 WP_000151588 IncI1-type conjugal transfer protein TrbB trbB
E9211_RS00435 (E9211_49450) 24261..25469 - 1209 WP_000121263 IncI1-type conjugal transfer protein TrbA trbA
E9211_RS00430 (E9211_49460) 25776..26555 - 780 WP_275450201 protein FinQ -
E9211_RS00425 (E9211_49470) 27182..27334 + 153 WP_001387489 Hok/Gef family protein -
E9211_RS00420 (E9211_49480) 27406..27657 - 252 WP_001291964 hypothetical protein -
E9211_RS32495 28158..28253 + 96 WP_000609148 DinQ-like type I toxin DqlB -
E9211_RS31800 (E9211_49500) 28318..28494 - 177 WP_001054898 hypothetical protein -
E9211_RS00405 (E9211_49510) 28677..28886 - 210 WP_001140543 hemolysin expression modulator Hha -
E9211_RS00400 (E9211_49520) 28984..29598 - 615 WP_000578648 plasmid IncI1-type surface exclusion protein ExcA -
E9211_RS00395 (E9211_49530) 29674..31842 - 2169 WP_000698360 IncI1-type conjugal transfer membrane protein TraY traY
E9211_RS00390 (E9211_49540) 31939..32523 - 585 WP_001037983 IncI1-type conjugal transfer protein TraX -
E9211_RS00385 (E9211_49550) 32552..33754 - 1203 WP_001387490 IncI1-type conjugal transfer protein TraW traW
E9211_RS00380 (E9211_49560) 33721..34335 - 615 WP_000337395 IncI1-type conjugal transfer protein TraV traV
E9211_RS00375 (E9211_49570) 34335..37379 - 3045 WP_001024789 IncI1-type conjugal transfer protein TraU traU
E9211_RS00370 (E9211_49580) 37469..38269 - 801 WP_001164783 IncI1-type conjugal transfer protein TraT traT
E9211_RS00365 (E9211_49590) 38253..38441 - 189 WP_001277253 putative conjugal transfer protein TraS -
E9211_RS00360 (E9211_49600) 38505..38909 - 405 WP_000086965 IncI1-type conjugal transfer protein TraR traR
E9211_RS00355 (E9211_49610) 38960..39487 - 528 WP_001055569 conjugal transfer protein TraQ traQ
E9211_RS00350 (E9211_49620) 39487..40191 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
E9211_RS00345 (E9211_49630) 40191..41480 - 1290 WP_001271994 conjugal transfer protein TraO traO
E9211_RS00340 (E9211_49640) 41483..42466 - 984 WP_001191879 IncI1-type conjugal transfer protein TraN traN
E9211_RS00335 (E9211_49650) 42477..43169 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
E9211_RS00330 (E9211_49660) 43166..43513 - 348 WP_001055900 conjugal transfer protein traL
E9211_RS00325 (E9211_49670) 43531..47295 - 3765 WP_001141540 LPD7 domain-containing protein -
E9211_RS00320 (E9211_49680) 47385..47936 - 552 WP_000014586 phospholipase D family protein -
E9211_RS29685 (E9211_49690) 47951..48241 - 291 WP_001314267 hypothetical protein traK
E9211_RS00315 (E9211_49700) 48238..49386 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
E9211_RS00310 (E9211_49710) 49383..50201 - 819 WP_000646098 IncI1-type conjugal transfer lipoprotein TraI traI
E9211_RS00305 (E9211_49720) 50198..50656 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
E9211_RS00300 (E9211_49730) 51051..51635 - 585 WP_000977522 histidine phosphatase family protein -
E9211_RS00295 (E9211_49740) 51695..52897 - 1203 WP_000976357 conjugal transfer protein TraF -
E9211_RS00290 (E9211_49750) 52983..53807 - 825 WP_001238932 conjugal transfer protein TraE traE
E9211_RS00285 (E9211_49760) 53958..55112 - 1155 WP_001139956 site-specific integrase -

Host bacterium

ID   2092 GenBank   NZ_AHAU01000146
Plasmid name   pE9211p1 Incompatibility group   -
Plasmid size   55139 bp Coordinate of oriT [Strand]   17706..17794 [-]
Host baterium   Escherichia coli O104:H4 str. E92/11

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrVA2