Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101642
Name   oriT_pMCR-E2899 in_silico
Organism   Escherichia coli strain MSHS 472
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JABFOO010000006 (51677..51765 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pMCR-E2899
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCCGTTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1432 GenBank   WP_052938718
Name   nikB_HN009_RS22795_pMCR-E2899 insolico UniProt ID   A0A706EBF4
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 104042.50 Da        Isoelectric Point: 7.3623

>WP_052938718.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVR
HTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSHMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLAERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWLPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A706EBF4


Auxiliary protein

ID   563 GenBank   WP_052938717
Name   WP_052938717_pMCR-E2899 insolico UniProt ID   A0A706B4V0
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12643.59 Da        Isoelectric Point: 10.7463

>WP_052938717.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSTVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A706B4V0


T4CP

ID   1098 GenBank   WP_021497839
Name   trbC_HN009_RS22800_pMCR-E2899 insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86967.19 Da        Isoelectric Point: 7.0662

>WP_021497839.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLKCADPSQDRMIKRSLFSFWPTLFQYEVILESPANGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 57143..97636

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HN009_RS22800 (HN009_22805) 54859..57150 - 2292 WP_021497839 F-type conjugative transfer protein TrbC -
HN009_RS22805 (HN009_22810) 57143..58213 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
HN009_RS22810 (HN009_22815) 58232..59440 - 1209 WP_000121273 IncI1-type conjugal transfer protein TrbA trbA
HN009_RS22815 (HN009_22820) 59732..59884 + 153 WP_001387489 Hok/Gef family protein -
HN009_RS22820 (HN009_22825) 59956..60207 - 252 WP_001291964 hypothetical protein -
HN009_RS22825 (HN009_22830) 60527..62098 - 1572 WP_171993024 IS66-like element ISCro1 family transposase -
HN009_RS22830 (HN009_22835) 62118..62465 - 348 WP_000624622 IS66 family insertion sequence element accessory protein TnpB -
HN009_RS22835 (HN009_22840) 62465..63142 - 678 WP_001682408 IS66-like element accessory protein TnpA -
HN009_RS24185 63413..63508 + 96 WP_001303310 DinQ-like type I toxin DqlB -
HN009_RS22840 (HN009_22845) 63573..63749 - 177 WP_001054898 hypothetical protein -
HN009_RS22845 (HN009_22850) 64149..64358 + 210 WP_000062603 HEAT repeat domain-containing protein -
HN009_RS22850 (HN009_22855) 64430..65080 - 651 WP_001178506 plasmid IncI1-type surface exclusion protein ExcA -
HN009_RS22855 (HN009_22860) 65154..67322 - 2169 WP_052938719 DotA/TraY family protein traY
HN009_RS22860 (HN009_22865) 67419..68003 - 585 WP_001476649 IncI1-type conjugal transfer protein TraX -
HN009_RS22865 (HN009_22870) 68032..69234 - 1203 WP_014065727 IncI1-type conjugal transfer protein TraW traW
HN009_RS22870 (HN009_22875) 69201..69815 - 615 WP_000337398 IncI1-type conjugal transfer protein TraV traV
HN009_RS22875 (HN009_22880) 69815..72859 - 3045 WP_001024782 IncI1-type conjugal transfer protein TraU traU
HN009_RS22880 (HN009_22885) 72949..73749 - 801 WP_021497829 IncI1-type conjugal transfer protein TraT traT
HN009_RS22885 (HN009_22890) 73733..73921 - 189 WP_001277255 putative conjugal transfer protein TraS -
HN009_RS22890 (HN009_22895) 73985..74389 - 405 WP_021497835 IncI1-type conjugal transfer protein TraR traR
HN009_RS22895 (HN009_22900) 74440..74967 - 528 WP_001055569 conjugal transfer protein TraQ traQ
HN009_RS22900 (HN009_22905) 74967..75671 - 705 WP_001493811 IncI1-type conjugal transfer protein TraP traP
HN009_RS22905 (HN009_22910) 75671..76960 - 1290 WP_001271991 conjugal transfer protein TraO traO
HN009_RS22910 (HN009_22915) 76963..77946 - 984 WP_171993026 IncI1-type conjugal transfer protein TraN traN
HN009_RS22915 (HN009_22920) 77957..78649 - 693 WP_000138548 DotI/IcmL family type IV secretion protein traM
HN009_RS22920 (HN009_22925) 78646..78993 - 348 WP_001055900 conjugal transfer protein traL
HN009_RS22925 (HN009_22930) 79011..82778 - 3768 WP_171993027 LPD7 domain-containing protein -
HN009_RS24230 82868..82951 - 84 Protein_95 phospholipase D family protein -
HN009_RS22930 (HN009_22935) 83039..83419 + 381 WP_001171523 transposase -
HN009_RS22935 (HN009_22940) 83416..83763 + 348 WP_000612556 IS66 family insertion sequence element accessory protein TnpB -
HN009_RS22940 (HN009_22945) 83859..85451 - 1593 WP_001593684 IS66 family transposase -
HN009_RS22945 (HN009_22950) 85482..85832 - 351 WP_000624717 IS66 family insertion sequence element accessory protein TnpB -
HN009_RS22950 (HN009_22955) 85829..86254 - 426 WP_000422741 transposase -
HN009_RS22955 (HN009_22960) 86269..86820 - 552 WP_252409331 phospholipase D family protein -
HN009_RS22960 (HN009_22965) 86835..87125 - 291 WP_001299214 hypothetical protein traK
HN009_RS22965 (HN009_22970) 87122..88270 - 1149 WP_001024972 plasmid transfer ATPase TraJ virB11
HN009_RS22970 (HN009_22975) 88267..89085 - 819 WP_032220737 IncI1-type conjugal transfer lipoprotein TraI traI
HN009_RS22975 (HN009_22980) 89082..89540 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
HN009_RS24060 89935..90096 - 162 Protein_106 histidine phosphatase family protein -
HN009_RS22985 (HN009_22990) 91606..91854 + 249 WP_001349157 hypothetical protein -
HN009_RS22990 (HN009_22995) 91851..93143 - 1293 WP_001619585 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
HN009_RS22995 (HN009_23000) 93143..93799 - 657 WP_001193549 A24 family peptidase -
HN009_RS23000 (HN009_23005) 93784..94344 - 561 WP_000014005 lytic transglycosylase domain-containing protein virB1
HN009_RS23005 (HN009_23010) 94354..94968 - 615 WP_000908226 type 4 pilus major pilin -
HN009_RS23010 (HN009_23015) 94985..96070 - 1086 WP_001208802 type II secretion system F family protein -
HN009_RS23015 (HN009_23020) 96083..97636 - 1554 WP_000362206 ATPase, T2SS/T4P/T4SS family virB11
HN009_RS23020 (HN009_23025) 97647..98099 - 453 WP_001247337 type IV pilus biogenesis protein PilP -
HN009_RS23025 (HN009_23030) 98086..99381 - 1296 WP_000752780 type 4b pilus protein PilO2 -
HN009_RS23030 (HN009_23035) 99374..101056 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
HN009_RS23035 (HN009_23040) 101070..101507 - 438 WP_000539811 type IV pilus biogenesis protein PilM -
HN009_RS23040 (HN009_23045) 101507..102574 - 1068 WP_054170954 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   2086 GenBank   NZ_JABFOO010000006
Plasmid name   pMCR-E2899 Incompatibility group   IncI1
Plasmid size   107399 bp Coordinate of oriT [Strand]   51677..51765 [-]
Host baterium   Escherichia coli strain MSHS 472

Cargo genes

Drug resistance gene   blaTEM-1B, mcr-1.1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -