Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 101634
• Name: oriT_CP4|p3
• Organism: Enterococcus faecium strain CP4
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_JAMWMK010000146 (26367..26466 [+], 100 nt)
• oriT length: 100 nt
• IRs (inverted repeats): 13..18, 32..37 (AAAGGG..CCCTTT)
• Location of nic site: 94..95
• Conserved sequence flanking the nic site: GCTTGCAGTA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 100 nt

>oriT_CP4|p3
CCGAAGGGGATGAAAGGGAACGTGTAACTTGCCCTTTTCCAAGCAACTTTGTCATACAAGACACTTCGTTATTGTATGACAAAGTGAAAGCTTGCAGTAT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_M3X98_RS15375_CP4|p3

• ID: 1430
• GenBank: WP_258863622
• Name: Relaxase_M3X98_RS15375_CP4|p3
• UniProt ID: _
• Length: 342 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 342 a.a.
• Molecular weight: 40259.66 Da
• Isoelectric Point: 10.2510

>WP_258863622.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterococcus]
MHNKTQERNQVLRITQSFALDELSPKNTQDWQRANDLGVALAEKLYPQHQTAVYTHLDGQNHVLHNHILV
NKVNLETGKKLREVPGKTVEKARVINDRLAQQQHWRILPPPQERFSETEQDLVAKQKYSYMDDLRTRINE
AMVDISVRSYKDLRNILEQKSIIFQERVQSLSYAFLDVNNKKRRAKGARLGTDFEKETIFNELENRARQQ
QPRPIEPVKQRELATTRLMQDTQQTEPSLERRESTTRGIEQQLEQRKPGIARLIDTIQHLRDQLPGITQL
VKKHLTYAKEALFDAFEKRFSFDMAQHKQVQRKKIQQDLEQRTQEQAMPKFQTPTQDRGLSL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (1-205)

Protein structure

No available structure.

Host bacterium

• ID: 2078
• GenBank: NZ_JAMWMK010000146
• Plasmid name: CP4|p3
• Incompatibility group: -
• Plasmid size: 35098 bp
• Coordinate of oriT [Strand]: 26367..26466 [+]
• Host baterium: Enterococcus faecium strain CP4

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21, AcrIIA1