Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101605 |
| Name | oriT_pEW671  |
| Organism | Klebsiella pneumoniae strain EW671 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JAQIIR010000001 (37251..37845 [+], 595 nt) |
| oriT length | 595 nt |
| IRs (inverted repeats) | 569..574, 579..584 (ATGCCC..GGGCAT) 497..503, 508..514 (CCTCCCG..CGGGAGG) 433..438, 442..447 (GTTCGC..GCGAAC) 75..80, 86..91 (TGCTTT..AAAGCA) 69..74, 82..87 (TTACTT..AAGTAA) |
| Location of nic site | 294..295 |
| Conserved sequence flanking the nic site |
TCCTGCATCG |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 595 nt
>oriT_pEW671
TGTTAAATACATTGTAGTTTTGATAAAATCATTAGTATCTGGCATAAAGTTATCATTATCATCTTCTGTTACTTTGCTTTTAAGTAAAGCACTGGCAAGCAATTTCATGCCTTTAGCAACAGTTAAACCAGGCTCATCTGGATAAATAACCTTCAGACATTCAAGTAGATTATCATGCTGTTTTTCTTCTAGTCTGAAATTAATTTGCTTCATAAATCCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAACGAGTAAAATGCTAGCATTTTGCGGCGTTTGCCCTATCCTGCATCGCTTTGATCTAGCACTAGTCTTGAATTGTGCTTCAGCGTCGAGGAAAAAGCAAAACTGAAGGGCTGTCATGGCTGAAGGTTTCGCTTGTCTACGACCGGAAACATCTCAATGGCTGTGAGCTATGTGGGATAAGGTTCGCTACGCGAACGGTCTGGCAGGGGGCGCAAGCGCTGTCTTGCAGAGTATATAAAAAAGCACCTCCCGCAAACGGGAGGGCTTCGGCGATTCATGACGTGTGAAACTATTCAGGGTCTGGTGTGTCGCCTACCATGCCCTGACGGGCATCACACAACCAG
TGTTAAATACATTGTAGTTTTGATAAAATCATTAGTATCTGGCATAAAGTTATCATTATCATCTTCTGTTACTTTGCTTTTAAGTAAAGCACTGGCAAGCAATTTCATGCCTTTAGCAACAGTTAAACCAGGCTCATCTGGATAAATAACCTTCAGACATTCAAGTAGATTATCATGCTGTTTTTCTTCTAGTCTGAAATTAATTTGCTTCATAAATCCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAACGAGTAAAATGCTAGCATTTTGCGGCGTTTGCCCTATCCTGCATCGCTTTGATCTAGCACTAGTCTTGAATTGTGCTTCAGCGTCGAGGAAAAAGCAAAACTGAAGGGCTGTCATGGCTGAAGGTTTCGCTTGTCTACGACCGGAAACATCTCAATGGCTGTGAGCTATGTGGGATAAGGTTCGCTACGCGAACGGTCTGGCAGGGGGCGCAAGCGCTGTCTTGCAGAGTATATAAAAAAGCACCTCCCGCAAACGGGAGGGCTTCGGCGATTCATGACGTGTGAAACTATTCAGGGTCTGGTGTGTCGCCTACCATGCCCTGACGGGCATCACACAACCAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1421 | GenBank | WP_044864205 |
| Name | mobP1_PGL11_RS00215_pEW671 |
UniProt ID | A0A221ZPJ2 |
| Length | 386 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 386 a.a. Molecular weight: 44352.85 Da Isoelectric Point: 10.6126
>WP_044864205.1 MULTISPECIES: MobP1 family relaxase [Enterobacterales]
MGVSVDKEYRVKRKSSEAGRKSAFAHKVKNGGKNYQRNVQERINRKGASKEVVVKISGGAITRQGIRNSI
DYMSRESELPVMSESGQVWKGDEIQEAKEHMVDRANDPQNVMNDKGKENKKVTQNIVFSPPVSAKVKPED
LLESVRKTMHKKYPNHRFVLGYHNDKKDHPHVHVVFRIRDEDGKRTDIRKKDLREIRTGFCEELKLKGYD
VKATHRQQQGLNQSIRDAHSTAPKRQKGVYEVVDVGYDHYQHDKTKPKQHFLKLKTLNKGVEKTYWGADF
GDLTSRENVKKGDLVKLKKLGQKEVKIPALDKHGVQHGWKTVHRNEWKLDNLGVKGIDRTPSSSKELVLN
SAAMLQKQQLQMKQFIQIKQAMLQNEQKMKIGIKLG
MGVSVDKEYRVKRKSSEAGRKSAFAHKVKNGGKNYQRNVQERINRKGASKEVVVKISGGAITRQGIRNSI
DYMSRESELPVMSESGQVWKGDEIQEAKEHMVDRANDPQNVMNDKGKENKKVTQNIVFSPPVSAKVKPED
LLESVRKTMHKKYPNHRFVLGYHNDKKDHPHVHVVFRIRDEDGKRTDIRKKDLREIRTGFCEELKLKGYD
VKATHRQQQGLNQSIRDAHSTAPKRQKGVYEVVDVGYDHYQHDKTKPKQHFLKLKTLNKGVEKTYWGADF
GDLTSRENVKKGDLVKLKKLGQKEVKIPALDKHGVQHGWKTVHRNEWKLDNLGVKGIDRTPSSSKELVLN
SAAMLQKQQLQMKQFIQIKQAMLQNEQKMKIGIKLG
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A221ZPJ2 |
T4CP
| ID | 1080 | GenBank | WP_060589145 |
| Name | t4cp2_PGL11_RS00155_pEW671 |
UniProt ID | _ |
| Length | 611 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 611 a.a. Molecular weight: 69235.94 Da Isoelectric Point: 7.1493
>WP_060589145.1 type IV secretory system conjugative DNA transfer family protein [Klebsiella pneumoniae]
MSLKLPDKGQWLFIGFVMCLFTYYAGSVAVYFFNGKTPLFIWKNFDSMLLWRLMTESNIRSDIRITAMPA
LLSGLAASVIVPVFIIWQLNKKDFSLYGDAKFASDDDLKKSKLLKWEKENDDDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREVILQNKVFLLDPFNSKTHKFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYAPDWLNEFNLKPIFSI
GSVVDLYSNIDRENVLANREDFEGIAGLDETALFHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDISIYVGVNAEDMSLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMSIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAKKIS
EKLGYTTAKSTGSSKTSGRSTSKSNSESDAQRALVLPQELGTLEFSEEFIILKGENPVKAKKALYYLDSY
FMDRLMLVSPKLAALTSSLNKTKNIFGVKGLKYPSKEKMLSVGELESEVLL
MSLKLPDKGQWLFIGFVMCLFTYYAGSVAVYFFNGKTPLFIWKNFDSMLLWRLMTESNIRSDIRITAMPA
LLSGLAASVIVPVFIIWQLNKKDFSLYGDAKFASDDDLKKSKLLKWEKENDDDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREVILQNKVFLLDPFNSKTHKFNPLF
YIDLKAESGAKDLLKLIEILFPSYGMTGAEAHFNNLAGQYWTGLAKLLHFFINYAPDWLNEFNLKPIFSI
GSVVDLYSNIDRENVLANREDFEGIAGLDETALFHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDISIYVGVNAEDMSLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMSIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAVSEEDDAKKIS
EKLGYTTAKSTGSSKTSGRSTSKSNSESDAQRALVLPQELGTLEFSEEFIILKGENPVKAKKALYYLDSY
FMDRLMLVSPKLAALTSSLNKTKNIFGVKGLKYPSKEKMLSVGELESEVLL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 24726..34681
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PGL11_RS00125 (PGL11_00125) | 20285..21058 | - | 774 | WP_044715185 | hypothetical protein | - |
| PGL11_RS00130 (PGL11_00130) | 21073..21543 | - | 471 | WP_044715183 | thermonuclease family protein | - |
| PGL11_RS00135 (PGL11_00135) | 21540..21875 | - | 336 | WP_050886236 | hypothetical protein | - |
| PGL11_RS00140 (PGL11_00140) | 21881..22084 | - | 204 | WP_044715181 | hypothetical protein | - |
| PGL11_RS00145 (PGL11_00145) | 22153..22491 | - | 339 | WP_225622178 | hypothetical protein | - |
| PGL11_RS00150 (PGL11_00150) | 22496..22891 | - | 396 | WP_044715177 | cag pathogenicity island Cag12 family protein | - |
| PGL11_RS00155 (PGL11_00155) | 22888..24723 | - | 1836 | WP_060589145 | type IV secretory system conjugative DNA transfer family protein | - |
| PGL11_RS00160 (PGL11_00160) | 24726..25757 | - | 1032 | WP_060589148 | P-type DNA transfer ATPase VirB11 | virB11 |
| PGL11_RS00165 (PGL11_00165) | 25781..26992 | - | 1212 | WP_044715172 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| PGL11_RS00170 (PGL11_00170) | 26989..27918 | - | 930 | WP_044715170 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| PGL11_RS00175 (PGL11_00175) | 27923..28651 | - | 729 | WP_044715168 | type IV secretion system protein | virB8 |
| PGL11_RS00180 (PGL11_00180) | 28891..29946 | - | 1056 | WP_060589144 | type IV secretion system protein | virB6 |
| PGL11_RS00185 (PGL11_00185) | 29958..30215 | - | 258 | WP_044715163 | EexN family lipoprotein | - |
| PGL11_RS00190 (PGL11_00190) | 30225..30971 | - | 747 | WP_044715162 | type IV secretion system protein | - |
| PGL11_RS00195 (PGL11_00195) | 30982..33741 | - | 2760 | WP_271048813 | VirB3 family type IV secretion system protein | virb4 |
| PGL11_RS00200 (PGL11_00200) | 33763..34053 | - | 291 | WP_044715156 | TrbC/VirB2 family protein | virB2 |
| PGL11_RS00205 (PGL11_00205) | 34037..34681 | - | 645 | WP_044715155 | lytic transglycosylase domain-containing protein | virB1 |
| PGL11_RS00210 (PGL11_00210) | 34905..35396 | - | 492 | WP_044715152 | transcription termination/antitermination NusG family protein | - |
| PGL11_RS00215 (PGL11_00215) | 35752..36912 | - | 1161 | WP_044864205 | MobP1 family relaxase | - |
| PGL11_RS00220 (PGL11_00220) | 36915..37463 | - | 549 | WP_044715148 | DNA distortion polypeptide 1 | - |
| PGL11_RS00225 (PGL11_00225) | 37791..38048 | - | 258 | WP_044715147 | hypothetical protein | - |
| PGL11_RS00230 (PGL11_00230) | 38064..38213 | - | 150 | WP_162852120 | hypothetical protein | - |
| PGL11_RS00235 (PGL11_00235) | 38291..38632 | - | 342 | WP_059347451 | hypothetical protein | - |
| PGL11_RS00240 (PGL11_00240) | 38729..38968 | - | 240 | WP_044715143 | hypothetical protein | - |
| PGL11_RS00245 (PGL11_00245) | 38961..39227 | - | 267 | WP_044715141 | hypothetical protein | - |
Host bacterium
| ID | 2049 | GenBank | NZ_JAQIIR010000001 |
| Plasmid name | pEW671 | Incompatibility group | IncX5 |
| Plasmid size | 41872 bp | Coordinate of oriT [Strand] | 37251..37845 [+] |
| Host baterium | Klebsiella pneumoniae strain EW671 |
Cargo genes
| Drug resistance gene | blaKPC-2 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |