Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101598 |
| Name | oriT_pCrieF1144_3  |
| Organism | Escherichia coli strain CrieF1144 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JBAGRT010000005 (13460..13512 [-], 53 nt) |
| oriT length | 53 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 53 nt
>oriT_pCrieF1144_3
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
CACACGATTGTAACATGACCGGAACGGTCTTGTGTACAATCGGTATCGTGCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 1073 | GenBank | WP_000338974 |
| Name | t4cp2_V5G27_RS26310_pCrieF1144_3 |
UniProt ID | _ |
| Length | 652 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 652 a.a. Molecular weight: 73347.89 Da Isoelectric Point: 9.1391
>WP_000338974.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLKWDTFHSVISTFSGNPQYKKLVFMAWF
GFSVPLIFFAIFMLIVVIGIMPKKVIYGDARLATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQF
IYFAGQQFLILYAPTRSGKGVGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAE
TIDQAIKGQIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDKERFH
LEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFFFKFMSAPDRTRGSIET
NFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPDALITHEKIVNLFFSLLVNENCRELPEHN
PDLKYQCLILLDEFTSMGKSEVIERAVGFTAGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLY
YPPKSKNALAKKISEEIGVRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNI
AIREIITSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKAGGKKVM
VAGGNVITNPDLDNHDKTDVSE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 31436..54606
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| V5G27_RS26245 (V5G27_26245) | 27828..28199 | - | 372 | WP_176478117 | pilus assembly protein | - |
| V5G27_RS26250 (V5G27_26250) | 28208..28363 | + | 156 | WP_001358489 | hypothetical protein | - |
| V5G27_RS26255 (V5G27_26255) | 29000..29326 | + | 327 | WP_333482995 | hypothetical protein | - |
| V5G27_RS26260 (V5G27_26260) | 29740..30784 | - | 1045 | Protein_38 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| V5G27_RS26265 (V5G27_26265) | 30797..31432 | - | 636 | WP_000934979 | A24 family peptidase | - |
| V5G27_RS26270 (V5G27_26270) | 31436..31918 | - | 483 | WP_001258095 | lytic transglycosylase domain-containing protein | virB1 |
| V5G27_RS26275 (V5G27_26275) | 31984..32541 | - | 558 | WP_000095048 | type 4 pilus major pilin | - |
| V5G27_RS26280 (V5G27_26280) | 32586..33695 | - | 1110 | WP_000974903 | type II secretion system F family protein | - |
| V5G27_RS26285 (V5G27_26285) | 33686..35224 | - | 1539 | WP_000466225 | ATPase, T2SS/T4P/T4SS family | virB11 |
| V5G27_RS26290 (V5G27_26290) | 35249..35743 | - | 495 | WP_000912553 | type IV pilus biogenesis protein PilP | - |
| V5G27_RS26295 (V5G27_26295) | 35727..37037 | - | 1311 | WP_001454111 | type 4b pilus protein PilO2 | - |
| V5G27_RS26300 (V5G27_26300) | 37088..38731 | - | 1644 | WP_001035589 | PilN family type IVB pilus formation outer membrane protein | - |
| V5G27_RS26305 (V5G27_26305) | 38724..39242 | - | 519 | WP_010895890 | sigma 54-interacting transcriptional regulator | virb4 |
| V5G27_RS26310 (V5G27_26310) | 39289..41247 | - | 1959 | WP_000338974 | type IV secretory system conjugative DNA transfer family protein | - |
| V5G27_RS26315 (V5G27_26315) | 41263..42318 | - | 1056 | WP_001059977 | P-type DNA transfer ATPase VirB11 | virB11 |
| V5G27_RS26320 (V5G27_26320) | 42377..43516 | - | 1140 | WP_000790641 | TrbI/VirB10 family protein | virB10 |
| V5G27_RS26325 (V5G27_26325) | 43506..44207 | - | 702 | WP_000274524 | TrbG/VirB9 family P-type conjugative transfer protein | virB9 |
| V5G27_RS26330 (V5G27_26330) | 44273..45007 | - | 735 | WP_000432283 | type IV secretion system protein | virB8 |
| V5G27_RS26335 (V5G27_26335) | 45007..45141 | - | 135 | WP_000701233 | hypothetical protein | - |
| V5G27_RS26340 (V5G27_26340) | 45173..47530 | - | 2358 | WP_023223346 | VirB4 family type IV secretion system protein | virb4 |
| V5G27_RS26345 (V5G27_26345) | 47536..47856 | - | 321 | WP_010895893 | VirB3 family type IV secretion system protein | virB3 |
| V5G27_RS26350 (V5G27_26350) | 47927..48217 | - | 291 | WP_000865478 | TrbC/VirB2 family protein | virB2 |
| V5G27_RS26355 (V5G27_26355) | 48217..48801 | - | 585 | WP_023223347 | lytic transglycosylase domain-containing protein | virB1 |
| V5G27_RS26360 (V5G27_26360) | 48822..49220 | - | 399 | WP_001153669 | hypothetical protein | - |
| V5G27_RS26365 (V5G27_26365) | 49560..49997 | - | 438 | WP_000539666 | type IV pilus biogenesis protein PilM | - |
| V5G27_RS26370 (V5G27_26370) | 50003..51238 | - | 1236 | WP_000733400 | toxin co-regulated pilus biosynthesis Q family protein | - |
| V5G27_RS26375 (V5G27_26375) | 51241..51528 | - | 288 | WP_001326593 | TrbM/KikA/MpfK family conjugal transfer protein | - |
| V5G27_RS26380 (V5G27_26380) | 51700..52335 | - | 636 | WP_000835773 | hypothetical protein | - |
| V5G27_RS26385 (V5G27_26385) | 52408..52695 | - | 288 | WP_001032611 | EexN family lipoprotein | - |
| V5G27_RS26390 (V5G27_26390) | 52708..52962 | - | 255 | WP_001043555 | EexN family lipoprotein | - |
| V5G27_RS26395 (V5G27_26395) | 52964..53605 | - | 642 | WP_001425343 | type IV secretion system protein | - |
| V5G27_RS26400 (V5G27_26400) | 53611..54606 | - | 996 | WP_001028543 | type IV secretion system protein | virB6 |
| V5G27_RS26405 (V5G27_26405) | 54610..54867 | - | 258 | WP_000739144 | hypothetical protein | - |
| V5G27_RS26410 (V5G27_26410) | 54864..55166 | - | 303 | WP_001360345 | hypothetical protein | - |
| V5G27_RS26415 (V5G27_26415) | 55182..55397 | - | 216 | WP_001360344 | hypothetical protein | - |
| V5G27_RS26420 (V5G27_26420) | 55581..56042 | - | 462 | WP_001243166 | hypothetical protein | - |
| V5G27_RS26425 (V5G27_26425) | 56053..56223 | - | 171 | WP_000550720 | hypothetical protein | - |
| V5G27_RS26430 (V5G27_26430) | 56227..56670 | - | 444 | WP_000964330 | NfeD family protein | - |
| V5G27_RS26435 (V5G27_26435) | 57044..57997 | - | 954 | WP_072089442 | SPFH domain-containing protein | - |
| V5G27_RS26440 (V5G27_26440) | 58024..58200 | - | 177 | WP_000753049 | hypothetical protein | - |
| V5G27_RS26445 (V5G27_26445) | 58193..58408 | - | 216 | WP_001127357 | DUF1187 family protein | - |
| V5G27_RS26450 (V5G27_26450) | 58401..58853 | - | 453 | WP_000101552 | CaiF/GrlA family transcriptional regulator | - |
Host bacterium
| ID | 2042 | GenBank | NZ_JBAGRT010000005 |
| Plasmid name | pCrieF1144_3 | Incompatibility group | IncI2 |
| Plasmid size | 59984 bp | Coordinate of oriT [Strand] | 13460..13512 [-] |
| Host baterium | Escherichia coli strain CrieF1144 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |