Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101589
Name   oriT_pEW669-NDM-1 in_silico
Organism   Klebsiella quasipneumoniae subsp. quasipneumoniae strain EW669
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JBAFZS010000001 (5169..5786 [-], 618 nt)
oriT length   618 nt
IRs (inverted repeats)      589..594, 608..613  (TCCATG..CATGGA)
 592..597, 602..607  (ATGCCC..GGGCAT)
 520..527, 530..537  (CCTCCCGT..ACGGGAGG)
 455..460, 464..469  (ATTCGC..GCGAAT)
 197..202, 208..213  (TTCAAT..ATTGAA)
 85..90, 96..101  (TGCTTT..AAAGCA)
Location of nic site      315..316
Conserved sequence flanking the
  nic site    		  TCCTGCATCG
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 618 nt

>oriT_pEW669-NDM-1
TTCTTTGTTTCCCTGTCAAATACATTGTGGTTTTGATAAAATCATCATTGTCTTTTGACACATTAACATCTTCATTTTTAACCTTGCTTTTCAGTAAAGCATTGGCAAGCAGCTTCATTCCTTTTGCCACGGTTAAACCCGGTTCATCTGGATAAAGTGTTTTAAGACAATCAAGTAGATCATCATGTTGGCTTTCTTCAATCCTGAATTGAACTTTTTTCATAAAACTCTCCAACAAGAACCGACTGTAGGTCACCGGGCAAACGTTGCGGAATGGCGTCAGAGACGTCATTTTGCGGCGTTTGCCCTATCCTGCATCGCAGTGAACTACCTCCACACATAAATTGTGCTTCAGCGTCCATCGAAATGCAAAAAATGAAAGGCTATCGTGGCTGTGATCTGGTGCGTCTGCGACCGGAGACAACGGATTCGCGGCTAACTTGGGCGGAAATAAATTCGCTACGCGAATGGCCTGGCAGGGGGCGCGAGCGCTGTTTTACGGAATATACAAAAAAAGCACCTCCCGTAAACGGGAGGGCTTCGGCGATTCAGGAACGGGAATTTTATTCTGCCTCTGGTGTGTCGCCTTCCATGCCCTGCCGGGCATCATGGAGCCAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1410 GenBank   WP_000539528
Name   mobP1_V5H46_RS00060_pEW669-NDM-1 insolico UniProt ID   A0A5E1W1I9
Length   386 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 386 a.a.        Molecular weight: 44411.86 Da        Isoelectric Point: 10.6017

>WP_000539528.1 MULTISPECIES: MobP1 family relaxase [Enterobacteriaceae]
MGVYVDKEFRVKRKSSEAGRKSAFAHKVKNGGKNYQRNVQERINRKGASKEVVVKISGGAITRQGVRNSI
DYMSRESELPVMSESGQVWKGDEIQEAKEHMIDRANDPQNVFDDKGKENKKVTQNIVFSPPVSAKVKPED
LLESVRKTMHKKYPNHRFVLGYHNDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKMKGYD
VKATHKQQHGLNQSIKDAHKTAPKRQKGVYEVVDVGYDHYQNDKTKPKQHFIKLKTLNKGVEKTYWGADF
GELTTRENVKKGDLVKLKKLGQKEVKIPALDKNGVQHGWKTAHRNEWQLENLGVKGIDRISSSSKELVLN
SAEMIKKQQLQMRNFSQIKQSMIQSEQKVKIGIRLG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(90-240)


  Protein structure


Source ID Structure
AlphaFold DB A0A5E1W1I9


T4CP

ID   1065 GenBank   WP_000108726
Name   t4cp2_V5H46_RS00080_pEW669-NDM-1 insolico UniProt ID   _
Length   917 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 917 a.a.        Molecular weight: 104822.73 Da        Isoelectric Point: 6.2666

>WP_000108726.1 MULTISPECIES: VirB3 family type IV secretion system protein [Enterobacterales]
MSTVFKGLTRPALIRGLGVPLYPFLGMCVICVLLGVWIHEAMYALILPGWYAIKRVTKIDERFFDLLYLR
MQIKGNPLANKRFNAVHYAGSSYDAVDISKVDNFMKLKDQSSLEELIPYSSHITDNLIVTRNHDLLATWQ
IDGAYFECVDEADLALLTDQLNTLIRSFDGKPVTFYTHRIRVRKEVRPVFDSKIPFVNRVMNDYYESLSA
AEYFENKLYLTVCYKPFSAEDKVTHFLSRKKGNKNIFEEPINDMNEICGRLSTYLSRFHSRRLGLYEENN
IVYSEQLTLFQKLLSGRWQKVRVTNSPFYTYLGGKDLFFGNDAGQITASDHARYFRCIEIKDYFQETDAG
IFDALMYLPVEYVQTSSFTPIDKQSAIKALDDQIDKLEMTDDAAKSLLADLKVGLDMVSSGYISFGKSHQ
TLIVYADSPERLVKDTNIVTTTLEDLGLIVTYSTLSLGAAYFAQLPGNYTLRPRLSSISSLNFAEMESFH
NFFTGKEKGNTWGNNLITLGGSGNDIYNLNYHMTTEHQNYFGKNPTLGHTEILGTSNVGKTVVMMTKAFA
AQQFGTPESFPPERKLKKLTTVFFDKDRAAEPGIRSMGGAYFRVKEGEPTGWNPAALPPTKRNISFMKDL
VRLLCTLNSEPLDDYQNRLISDAVERLMQRSNRSYPISKLRPLILEPDDTETRRHGLKARLNAWVQGGEF
GWVFDNPDDTFDVDNLDVFGIDGTEFLDNKVLSSAASFYLIYRVTMLADGRRLLIYMDEFWQWINNEAFR
DFVYNKLKTARKLDMVLVVATQSPDELIKSPIAAAVREQCATHIYLANPKAKRSEYVDELEVRELYFDKI
KAIDPLSRQFLVVKNPQRKGESDDFAAFARLDLGKAAYYLPVLSASKPQLELFDEIWKEGMKPEEWLDTY
LERANLI

  Protein domains


Predicted by InterproScan.

Type IV secretory pathway, VirB3-like protein (VirB3)

(2-83)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(272-474)

  Protein structure



No available structure.



ID   1066 GenBank   WP_000053822
Name   t4cp2_V5H46_RS00125_pEW669-NDM-1 insolico UniProt ID   _
Length   611 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 611 a.a.        Molecular weight: 69204.04 Da        Isoelectric Point: 7.4342

>WP_000053822.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacterales]
MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIRTDIRLTAIPS
LLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFASDNDLRKSKLLKWEKENDTDILVGAYKGKYLWYTAPD
FVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKITSKVREKLLGNKVYLLDPFNSKTHQFNPLF
YIDLKEESGAKDLLKLIEILFPSYGLTGAEAHFNNLAGQYWTGLAKLLHFFINYDPSWLGEFGLKPVFSI
GSVVDLYSNIDRELILSKREDLEGTKGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYL
PTVRKCTDGNDFDLRQLRREDITVYVGVNAEDMSLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGVEGAKTLMSAHPCRIIYAVSEEDDAKKIS
EKLGYITTKSTGSSKTSGRSTSKSSSESEAQRALVLPQELGTLDFKEEFIILKGENPVKAEKALYFLDPY
FMDRLMMVSPKLTELTASLNKTKKVLGVKGLKYPSKEKMLSVGELESEVLL

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(102-572)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 8367..18476

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
V5H46_RS00030 (V5H46_00030) 3797..4066 + 270 WP_001006717 hypothetical protein -
V5H46_RS00035 (V5H46_00035) 4056..4298 + 243 WP_000008707 hypothetical protein -
V5H46_RS00040 (V5H46_00040) 4392..4730 + 339 WP_001099725 hypothetical protein -
V5H46_RS00045 (V5H46_00045) 4805..4969 + 165 WP_000004083 hypothetical protein -
V5H46_RS00050 (V5H46_00050) 4966..5223 + 258 WP_004199355 hypothetical protein -
V5H46_RS00055 (V5H46_00055) 5564..6109 + 546 WP_001348698 DNA distortion polypeptide 1 -
V5H46_RS00060 (V5H46_00060) 6112..7272 + 1161 WP_000539528 MobP1 family relaxase -
V5H46_RS00065 (V5H46_00065) 7625..8134 + 510 WP_000455437 transcription termination/antitermination NusG family protein -
V5H46_RS00070 (V5H46_00070) 8367..9011 + 645 WP_000953538 lytic transglycosylase domain-containing protein virB1
V5H46_RS00075 (V5H46_00075) 8995..9285 + 291 WP_000916156 TrbC/VirB2 family protein virB2
V5H46_RS00080 (V5H46_00080) 9310..12063 + 2754 WP_000108726 VirB3 family type IV secretion system protein virb4
V5H46_RS00085 (V5H46_00085) 12073..12843 + 771 WP_000716326 type IV secretion system protein -
V5H46_RS00090 (V5H46_00090) 12853..13110 + 258 WP_000759841 EexN family lipoprotein -
V5H46_RS00095 (V5H46_00095) 13122..14177 + 1056 WP_000235886 type IV secretion system protein virB6
V5H46_RS00100 (V5H46_00100) 14372..15100 + 729 WP_000505940 type IV secretion system protein virB8
V5H46_RS00105 (V5H46_00105) 15106..16035 + 930 WP_000783386 TrbG/VirB9 family P-type conjugative transfer protein virB9
V5H46_RS00110 (V5H46_00110) 16032..17246 + 1215 WP_001295060 VirB10/TraB/TrbI family type IV secretion system protein virB10
V5H46_RS00115 (V5H46_00115) 17248..17445 + 198 WP_000629109 DNA-binding protein -
V5H46_RS00120 (V5H46_00120) 17442..18476 + 1035 WP_000650512 P-type DNA transfer ATPase VirB11 virB11
V5H46_RS00125 (V5H46_00125) 18479..20314 + 1836 WP_000053822 type IV secretory system conjugative DNA transfer family protein -
V5H46_RS00130 (V5H46_00130) 20311..20703 + 393 WP_000738524 cag pathogenicity island Cag12 family protein -
V5H46_RS00135 (V5H46_00135) 20791..21105 + 315 WP_000754496 TrbM/KikA/MpfK family conjugal transfer protein -
V5H46_RS00140 (V5H46_00140) 21191..21517 + 327 WP_004208538 hypothetical protein -
V5H46_RS00145 (V5H46_00145) 21514..22008 + 495 WP_001215543 thermonuclease family protein -
V5H46_RS00150 (V5H46_00150) 22091..23353 + 1263 WP_000517490 ATP-binding protein -

Host bacterium

ID   2033 GenBank   NZ_JBAFZS010000001
Plasmid name   pEW669-NDM-1 Incompatibility group   IncX3
Plasmid size   50989 bp Coordinate of oriT [Strand]   5169..5786 [-]
Host baterium   Klebsiella quasipneumoniae subsp. quasipneumoniae strain EW669

Cargo genes

Drug resistance gene   blaNDM-1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -