Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   101585
Name   oriT_pMFDS1016229 in_silico
Organism   Escherichia coli strain MFDS1016229
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JAQMVH010000002 (90690..90814 [-], 125 nt)
oriT length   125 nt
IRs (inverted repeats)      102..107, 120..125  (TTTAAT..ATTAAA)
 92..100, 114..122  (AATAATGTA..TACATTATT)
 91..96, 108..113  (AAATAA..TTATTT)
 41..48, 61..68  (AAAAACAA..TTGTTTTT)
 39..46, 49..56  (GCAAAAAC..GTTTTTGC)
 3..10, 15..22  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 125 nt

>oriT_pMFDS1016229
GGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGTTTTTTTTAATCATTAGTTTATGTTCTAAATAATGTATTTTAATTTATTTTACATTATTAAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   1406 GenBank   WP_096136349
Name   traI_V6L98_RS24590_pMFDS1016229 insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 192263.11 Da        Isoelectric Point: 5.9188

>WP_096136349.1 conjugative transfer relaxase/helicase TraI [Escherichia coli]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGRNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLGERKVQFTYTDVLARTVGILPPE
NGVIERVRAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGRPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSNSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVSDSPFMALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYETEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLLQRSGETPNFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKSDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVELPAFDRNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLQ
DGRTEQAVREIAGQERERAVTSEREAALPESVLREPQREREAVREVVRENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

(969-1156)

(575-626)

(10-283)

(633-712)

(1434-1557)


  Protein structure



No available structure.




Auxiliary protein


ID   547 GenBank   WP_001254386
Name   WP_001254386_pMFDS1016229 insolico UniProt ID   A0A3Z6KJB5
Length   75 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 75 a.a.        Molecular weight: 9005.11 Da        Isoelectric Point: 10.1422

>WP_001254386.1 MULTISPECIES: conjugal transfer relaxosome protein TraY [Gammaproteobacteria]
MRRRNARGGISRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEIFREVTEESES
TFKEL

  Protein domains


Predicted by InterproScan.

(14-61)


  Protein structure


Source ID Structure
AlphaFold DB A0A3Z6KJB5


T4CP


ID   1059 GenBank   WP_062873762
Name   traD_V6L98_RS24605_pMFDS1016229 insolico UniProt ID   _
Length   717 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 717 a.a.        Molecular weight: 81434.90 Da        Isoelectric Point: 5.0485

>WP_062873762.1 type IV conjugative transfer system coupling protein TraD [Escherichia coli]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCTTLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAASLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQTRPKVAPEFIPRDINPDMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPVSPAI
NDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDIQQQMQRREEVN
INVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

(32-128)

(173-560)

  Protein structure



No available structure.



ID   1060 GenBank   WP_001064245
Name   traC_V6L98_RS24695_pMFDS1016229 insolico UniProt ID   _
Length   875 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 875 a.a.        Molecular weight: 99159.81 Da        Isoelectric Point: 5.8486

>WP_001064245.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacteriaceae]
MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAA
TQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNVADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIVLLDQYT
ANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR
LLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

(289-446)

(38-276)

(467-771)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 65231..88452

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
V6L98_RS24595 64597..64824 + 228 WP_000450532 toxin-antitoxin system antitoxin VapB -
V6L98_RS24600 64824..65222 + 399 WP_000911317 type II toxin-antitoxin system VapC family toxin -
V6L98_RS24605 65231..67384 - 2154 WP_062873762 type IV conjugative transfer system coupling protein TraD virb4
V6L98_RS24610 67636..68367 - 732 WP_024187508 conjugal transfer complement resistance protein TraT -
V6L98_RS24615 68381..68890 - 510 WP_032082913 conjugal transfer entry exclusion protein TraS -
V6L98_RS24620 68887..71712 - 2826 WP_062873763 conjugal transfer mating-pair stabilization protein TraG traG
V6L98_RS24625 71709..73082 - 1374 WP_000944319 conjugal transfer pilus assembly protein TraH traH
V6L98_RS24630 73069..73461 - 393 WP_032082924 F-type conjugal transfer protein TrbF -
V6L98_RS24635 73448..73789 - 342 WP_001442101 P-type conjugative transfer protein TrbJ -
V6L98_RS24640 73719..74264 - 546 WP_032082911 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
V6L98_RS24645 74251..74535 - 285 WP_000624108 type-F conjugative transfer system pilin chaperone TraQ -
V6L98_RS24650 74616..74891 + 276 WP_074430089 toxin ArtA -
V6L98_RS24655 74893..75240 - 348 WP_032082949 conjugal transfer protein TrbA -
V6L98_RS24660 75256..75999 - 744 WP_135460420 type-F conjugative transfer system pilin assembly protein TraF traF
V6L98_RS24665 75992..76249 - 258 WP_000864318 conjugal transfer protein TrbE -
V6L98_RS24670 76276..78084 - 1809 WP_032082947 type-F conjugative transfer system mating-pair stabilization protein TraN traN
V6L98_RS24675 78081..78719 - 639 WP_000777695 type-F conjugative transfer system pilin assembly protein TrbC trbC
V6L98_RS24680 78728..79720 - 993 WP_000830183 conjugal transfer pilus assembly protein TraU traU
V6L98_RS24685 79717..80349 - 633 WP_001203720 type-F conjugative transfer system protein TraW traW
V6L98_RS24690 80346..80732 - 387 WP_032082946 type-F conjugative transfer system protein TrbI -
V6L98_RS24695 80729..83356 - 2628 WP_001064245 type IV secretion system protein TraC virb4
V6L98_RS24700 83516..83737 - 222 WP_001278689 conjugal transfer protein TraR -
V6L98_RS24705 83872..84387 - 516 WP_000809838 type IV conjugative transfer system lipoprotein TraV traV
V6L98_RS24710 84384..84634 - 251 Protein_110 conjugal transfer protein TrbG -
V6L98_RS24715 84646..84843 - 198 WP_001324648 conjugal transfer protein TrbD -
V6L98_RS24720 84830..85420 - 591 WP_000002787 conjugal transfer pilus-stabilizing protein TraP -
V6L98_RS24725 85410..86837 - 1428 WP_000146685 F-type conjugal transfer pilus assembly protein TraB traB
V6L98_RS24730 86837..87566 - 730 Protein_114 type-F conjugative transfer system secretin TraK -
V6L98_RS24735 87553..88119 - 567 WP_000399792 type IV conjugative transfer system protein TraE traE
V6L98_RS24740 88141..88452 - 312 WP_000012106 type IV conjugative transfer system protein TraL traL
V6L98_RS24745 88467..88821 - 355 Protein_117 type IV conjugative transfer system pilin TraA -
V6L98_RS24750 88869..89096 - 228 WP_001254386 conjugal transfer relaxosome protein TraY -
V6L98_RS24755 89196..89883 - 688 Protein_119 PAS domain-containing protein -
V6L98_RS24760 90074..90459 - 386 Protein_120 conjugal transfer relaxosome DNA-binding protein TraM -
V6L98_RS24765 90784..91390 + 607 Protein_121 transglycosylase SLT domain-containing protein -
V6L98_RS24770 91687..92508 - 822 Protein_122 DUF932 domain-containing protein -
V6L98_RS24775 92615..92910 - 296 Protein_123 hypothetical protein -
V6L98_RS24780 92942..93064 - 123 Protein_124 single-stranded DNA-binding protein -
V6L98_RS24785 93134..93412 + 279 Protein_125 hypothetical protein -


Host bacterium


ID   2029 GenBank   NZ_JAQMVH010000002
Plasmid name   pMFDS1016229 Incompatibility group   IncFII
Plasmid size   94370 bp Coordinate of oriT [Strand]   90690..90814 [-]
Host baterium   Escherichia coli strain MFDS1016229

Cargo genes


Drug resistance gene   -
Virulence gene   estIa
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -