Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 101585 |
Name | oriT_pMFDS1016229 |
Organism | Escherichia coli strain MFDS1016229 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_JAQMVH010000002 (90690..90814 [-], 125 nt) |
oriT length | 125 nt |
IRs (inverted repeats) | 102..107, 120..125 (TTTAAT..ATTAAA) 92..100, 114..122 (AATAATGTA..TACATTATT) 91..96, 108..113 (AAATAA..TTATTT) 41..48, 61..68 (AAAAACAA..TTGTTTTT) 39..46, 49..56 (GCAAAAAC..GTTTTTGC) 3..10, 15..22 (TTGGTGGT..ACCACCAA) |
Location of nic site | _ |
Conserved sequence flanking the nic site |
_ |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 125 nt
GGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGTTTTTTTTAATCATTAGTTTATGTTCTAAATAATGTATTTTAATTTATTTTACATTATTAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 1406 | GenBank | WP_096136349 |
Name | traI_V6L98_RS24590_pMFDS1016229 | UniProt ID | _ |
Length | 1756 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 1756 a.a. Molecular weight: 192263.11 Da Isoelectric Point: 5.9188
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGRNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLGERKVQFTYTDVLARTVGILPPE
NGVIERVRAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGRPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSNSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVSDSPFMALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYETEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLLQRSGETPNFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKSDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVELPAFDRNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLQ
DGRTEQAVREIAGQERERAVTSEREAALPESVLREPQREREAVREVVRENLLQERLQQMERDMVRDLQKE
KTLGGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
ID | 547 | GenBank | WP_001254386 |
Name | WP_001254386_pMFDS1016229 | UniProt ID | A0A3Z6KJB5 |
Length | 75 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 75 a.a. Molecular weight: 9005.11 Da Isoelectric Point: 10.1422
MRRRNARGGISRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEIFREVTEESES
TFKEL
Protein domains
Predicted by InterproScan.
Protein structure
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A3Z6KJB5 |
T4CP
ID | 1059 | GenBank | WP_062873762 |
Name | traD_V6L98_RS24605_pMFDS1016229 | UniProt ID | _ |
Length | 717 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 717 a.a. Molecular weight: 81434.90 Da Isoelectric Point: 5.0485
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCTTLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAASLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQTRPKVAPEFIPRDINPDMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPVSPAI
NDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDIQQQMQRREEVN
INVHRERGEDVEPGDDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
ID | 1060 | GenBank | WP_001064245 |
Name | traC_V6L98_RS24695_pMFDS1016229 | UniProt ID | _ |
Length | 875 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 875 a.a. Molecular weight: 99159.81 Da Isoelectric Point: 5.8486
MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAA
TQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNVADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIVLLDQYT
ANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR
LLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 65231..88452
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
V6L98_RS24595 | 64597..64824 | + | 228 | WP_000450532 | toxin-antitoxin system antitoxin VapB | - |
V6L98_RS24600 | 64824..65222 | + | 399 | WP_000911317 | type II toxin-antitoxin system VapC family toxin | - |
V6L98_RS24605 | 65231..67384 | - | 2154 | WP_062873762 | type IV conjugative transfer system coupling protein TraD | virb4 |
V6L98_RS24610 | 67636..68367 | - | 732 | WP_024187508 | conjugal transfer complement resistance protein TraT | - |
V6L98_RS24615 | 68381..68890 | - | 510 | WP_032082913 | conjugal transfer entry exclusion protein TraS | - |
V6L98_RS24620 | 68887..71712 | - | 2826 | WP_062873763 | conjugal transfer mating-pair stabilization protein TraG | traG |
V6L98_RS24625 | 71709..73082 | - | 1374 | WP_000944319 | conjugal transfer pilus assembly protein TraH | traH |
V6L98_RS24630 | 73069..73461 | - | 393 | WP_032082924 | F-type conjugal transfer protein TrbF | - |
V6L98_RS24635 | 73448..73789 | - | 342 | WP_001442101 | P-type conjugative transfer protein TrbJ | - |
V6L98_RS24640 | 73719..74264 | - | 546 | WP_032082911 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
V6L98_RS24645 | 74251..74535 | - | 285 | WP_000624108 | type-F conjugative transfer system pilin chaperone TraQ | - |
V6L98_RS24650 | 74616..74891 | + | 276 | WP_074430089 | toxin ArtA | - |
V6L98_RS24655 | 74893..75240 | - | 348 | WP_032082949 | conjugal transfer protein TrbA | - |
V6L98_RS24660 | 75256..75999 | - | 744 | WP_135460420 | type-F conjugative transfer system pilin assembly protein TraF | traF |
V6L98_RS24665 | 75992..76249 | - | 258 | WP_000864318 | conjugal transfer protein TrbE | - |
V6L98_RS24670 | 76276..78084 | - | 1809 | WP_032082947 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
V6L98_RS24675 | 78081..78719 | - | 639 | WP_000777695 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
V6L98_RS24680 | 78728..79720 | - | 993 | WP_000830183 | conjugal transfer pilus assembly protein TraU | traU |
V6L98_RS24685 | 79717..80349 | - | 633 | WP_001203720 | type-F conjugative transfer system protein TraW | traW |
V6L98_RS24690 | 80346..80732 | - | 387 | WP_032082946 | type-F conjugative transfer system protein TrbI | - |
V6L98_RS24695 | 80729..83356 | - | 2628 | WP_001064245 | type IV secretion system protein TraC | virb4 |
V6L98_RS24700 | 83516..83737 | - | 222 | WP_001278689 | conjugal transfer protein TraR | - |
V6L98_RS24705 | 83872..84387 | - | 516 | WP_000809838 | type IV conjugative transfer system lipoprotein TraV | traV |
V6L98_RS24710 | 84384..84634 | - | 251 | Protein_110 | conjugal transfer protein TrbG | - |
V6L98_RS24715 | 84646..84843 | - | 198 | WP_001324648 | conjugal transfer protein TrbD | - |
V6L98_RS24720 | 84830..85420 | - | 591 | WP_000002787 | conjugal transfer pilus-stabilizing protein TraP | - |
V6L98_RS24725 | 85410..86837 | - | 1428 | WP_000146685 | F-type conjugal transfer pilus assembly protein TraB | traB |
V6L98_RS24730 | 86837..87566 | - | 730 | Protein_114 | type-F conjugative transfer system secretin TraK | - |
V6L98_RS24735 | 87553..88119 | - | 567 | WP_000399792 | type IV conjugative transfer system protein TraE | traE |
V6L98_RS24740 | 88141..88452 | - | 312 | WP_000012106 | type IV conjugative transfer system protein TraL | traL |
V6L98_RS24745 | 88467..88821 | - | 355 | Protein_117 | type IV conjugative transfer system pilin TraA | - |
V6L98_RS24750 | 88869..89096 | - | 228 | WP_001254386 | conjugal transfer relaxosome protein TraY | - |
V6L98_RS24755 | 89196..89883 | - | 688 | Protein_119 | PAS domain-containing protein | - |
V6L98_RS24760 | 90074..90459 | - | 386 | Protein_120 | conjugal transfer relaxosome DNA-binding protein TraM | - |
V6L98_RS24765 | 90784..91390 | + | 607 | Protein_121 | transglycosylase SLT domain-containing protein | - |
V6L98_RS24770 | 91687..92508 | - | 822 | Protein_122 | DUF932 domain-containing protein | - |
V6L98_RS24775 | 92615..92910 | - | 296 | Protein_123 | hypothetical protein | - |
V6L98_RS24780 | 92942..93064 | - | 123 | Protein_124 | single-stranded DNA-binding protein | - |
V6L98_RS24785 | 93134..93412 | + | 279 | Protein_125 | hypothetical protein | - |
Host bacterium
ID | 2029 | GenBank | NZ_JAQMVH010000002 |
Plasmid name | pMFDS1016229 | Incompatibility group | IncFII |
Plasmid size | 94370 bp | Coordinate of oriT [Strand] | 90690..90814 [-] |
Host baterium | Escherichia coli strain MFDS1016229 |
Cargo genes
Drug resistance gene | - |
Virulence gene | estIa |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |