Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 101569 |
Name | oriT_pMFDS1009773 |
Organism | Escherichia coli strain MFDS1009773 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_JAQMUO010000003 (218225..218348 [-], 124 nt) |
oriT length | 124 nt |
IRs (inverted repeats) | 92..99, 113..120 (ATAATGTA..TACATTAT) 90..95, 107..112 (AAATAA..TTATTT) 39..46, 49..56 (GCAAAAAC..GTTTTTGC) 3..10, 15..22 (TTGGTGGT..ACCACCAA) |
Location of nic site | _ |
Conserved sequence flanking the nic site |
_ |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
GGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGCTATTTGAATCATTAACTTATGTCTTAAATAATGTATTTTAATTTATTTTACATTATAAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 1391 | GenBank | WP_000986971 |
Name | traI_V6M11_RS26500_pMFDS1009773 | UniProt ID | _ |
Length | 1756 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 1756 a.a. Molecular weight: 192129.86 Da Isoelectric Point: 5.8508
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGKLPDGADLSRMQ
DGSNRHRPGYDLTFSAPKSVSMMAMPGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIRAAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAESRMQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTTGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELAMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLISDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKIPVAEGERLRVTGKIPGLRISGGDRLQVASVSEDAMTVVVPGRAEPASLPVGYSPFTALKL
ESGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVVESKNLAFSQVDLLTEAKSFAAEGTSFTDLGREINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETIDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDAIVLVDNVYHRIAGISRDDGLITLQDTDGNSRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVMAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIALGRKYPQPYVALPAFDRNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSEDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAEEKPDQP
DDKTEQAVRDIAGQERDRAVTSEREAVLPESVLREPQRVQDAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
ID | 517 | GenBank | WP_332831925 |
Name | WP_332831925_pMFDS1009773 | UniProt ID | _ |
Length | 75 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 75 a.a. Molecular weight: 9051.13 Da Isoelectric Point: 9.9851
MRRRNAKAGTSRTISVYLDEDTNNRLIRAKDRSGRSKTIEIQIRLRDHLKRYPDFYNEEIFREVTEESES
TFKEL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
ID | 518 | GenBank | WP_001151555 |
Name | WP_001151555_pMFDS1009773 | UniProt ID | _ |
Length | 127 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 127 a.a. Molecular weight: 14461.36 Da Isoelectric Point: 4.5221
MAKVQAYVSDEIVYKINEIVERRRAEGAKSTDVSFSSISTMLLELGLRVYEAQMERKESAFNQAEFNKVL
LECAIKTQSTVAKILGIESLSPHVSGNPKFEYANMVEDIRDKVSSEMERFFPENDEE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
ID | 1029 | GenBank | WP_000009375 |
Name | traD_V6M11_RS26515_pMFDS1009773 | UniProt ID | _ |
Length | 714 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 714 a.a. Molecular weight: 81137.72 Da Isoelectric Point: 5.1669
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKLSWQTFVNGCIYWWCTTLEGMR
DLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATIVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDLNPEMENRLSAVLAAREAEGRQMASLFEPEVVSGEDVTQAEQPQQPVSSVINDK
KSDAGVSVPAGGIAQELKMKPEEEMEQQLPPGISESGEVVDMAVYEAWQREQNPDIQQQMQRREEVNINV
HRERGEDVEPGDDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
ID | 1030 | GenBank | WP_001064271 |
Name | traC_V6M11_RS26630_pMFDS1009773 | UniProt ID | _ |
Length | 875 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 875 a.a. Molecular weight: 99434.22 Da Isoelectric Point: 5.9665
MNNPLEAVTQAVNSLVTALKLPDESSKANEILGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANETIVEALDHMLRTKLPRGIPLCIHLMSSQLVGERIKYGLREFSWSGEQAERFNAITRAYYMKAAE
TLFPLPEGLNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGAYITTQTVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLVKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIVLLDQYT
ANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR
LLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRQEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 189893..218920
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
V6M11_RS26505 | 189259..189486 | + | 228 | WP_000450526 | toxin-antitoxin system antitoxin VapB | - |
V6M11_RS26510 | 189486..189884 | + | 399 | WP_000911316 | type II toxin-antitoxin system VapC family toxin | - |
V6M11_RS26515 | 189893..192037 | - | 2145 | WP_000009375 | type IV conjugative transfer system coupling protein TraD | virb4 |
V6M11_RS26520 | 192093..192823 | - | 731 | Protein_212 | hypothetical protein | - |
V6M11_RS26525 | 193058..193537 | - | 480 | WP_001355324 | hypothetical protein | - |
V6M11_RS26530 | 193741..194472 | - | 732 | WP_001364068 | conjugal transfer complement resistance protein TraT | - |
V6M11_RS26535 | 194504..195007 | - | 504 | WP_000605506 | hypothetical protein | - |
V6M11_RS26540 | 195023..197866 | - | 2844 | WP_001006995 | conjugal transfer mating-pair stabilization protein TraG | traG |
V6M11_RS26545 | 197863..199236 | - | 1374 | WP_001137366 | conjugal transfer pilus assembly protein TraH | traH |
V6M11_RS26550 | 199223..199615 | - | 393 | WP_000138366 | F-type conjugal transfer protein TrbF | - |
V6M11_RS26555 | 199569..199883 | - | 315 | WP_001244895 | P-type conjugative transfer protein TrbJ | - |
V6M11_RS26560 | 199873..200409 | - | 537 | WP_000059854 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
V6M11_RS26565 | 200396..200680 | - | 285 | WP_000624195 | type-F conjugative transfer system pilin chaperone TraQ | - |
V6M11_RS26570 | 200761..201096 | + | 336 | WP_000415768 | hypothetical protein | - |
V6M11_RS26575 | 201077..201382 | - | 306 | WP_236922840 | conjugal transfer protein TrbA | - |
V6M11_RS26580 | 201427..202170 | - | 744 | WP_001030399 | type-F conjugative transfer system pilin assembly protein TraF | traF |
V6M11_RS26585 | 202163..202420 | - | 258 | WP_000864356 | conjugal transfer protein TrbE | - |
V6M11_RS26590 | 202434..204338 | - | 1905 | WP_000662057 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
V6M11_RS26595 | 204366..204584 | + | 219 | WP_001364024 | hypothetical protein | - |
V6M11_RS26600 | 204573..204953 | - | 381 | WP_000009645 | hypothetical protein | - |
V6M11_RS26605 | 204950..205588 | - | 639 | WP_000087541 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
V6M11_RS26610 | 205615..206134 | - | 520 | Protein_230 | hypothetical protein | - |
V6M11_RS26615 | 206410..207402 | - | 993 | WP_001355328 | conjugal transfer pilus assembly protein TraU | traU |
V6M11_RS26620 | 207399..208031 | - | 633 | WP_000650119 | type-F conjugative transfer system protein TraW | traW |
V6M11_RS26625 | 208028..208354 | - | 327 | WP_332831924 | type-F conjugative transfer system protein TrbI | - |
V6M11_RS26630 | 208412..211039 | - | 2628 | WP_001064271 | type IV secretion system protein TraC | virb4 |
V6M11_RS26635 | 211199..211420 | - | 222 | WP_001278688 | conjugal transfer protein TraR | - |
V6M11_RS26640 | 211555..212070 | - | 516 | WP_000802746 | type IV conjugative transfer system lipoprotein TraV | traV |
V6M11_RS26645 | 212067..212387 | - | 321 | WP_001057269 | DUF2689 domain-containing protein | - |
V6M11_RS26650 | 212374..212961 | - | 588 | WP_000002892 | conjugal transfer pilus-stabilizing protein TraP | - |
V6M11_RS26655 | 212951..214381 | - | 1431 | WP_000146619 | F-type conjugal transfer pilus assembly protein TraB | traB |
V6M11_RS26660 | 214381..215109 | - | 729 | WP_001355329 | type-F conjugative transfer system secretin TraK | traK |
V6M11_RS26665 | 215096..215662 | - | 567 | WP_000399757 | type IV conjugative transfer system protein TraE | traE |
V6M11_RS26670 | 215684..215995 | - | 312 | WP_000012121 | type IV conjugative transfer system protein TraL | traL |
V6M11_RS26675 | 216010..216369 | - | 360 | WP_000340277 | type IV conjugative transfer system pilin TraA | - |
V6M11_RS26680 | 216403..216630 | - | 228 | WP_332831925 | conjugal transfer relaxosome protein TraY | - |
V6M11_RS26685 | 216724..217410 | - | 687 | WP_000332479 | PAS domain-containing protein | - |
V6M11_RS26690 | 217604..217987 | - | 384 | WP_001151555 | conjugal transfer relaxosome DNA-binding protein TraM | - |
V6M11_RS26695 | 218273..218920 | + | 648 | WP_000614283 | transglycosylase SLT domain-containing protein | virB1 |
V6M11_RS26700 | 219217..220038 | - | 822 | Protein_248 | DUF932 domain-containing protein | - |
V6M11_RS26705 | 220157..220444 | - | 288 | WP_000107535 | hypothetical protein | - |
V6M11_RS26710 | 220695..221699 | + | 1005 | WP_332831926 | IS110 family transposase | - |
V6M11_RS26715 | 221793..222053 | - | 261 | WP_000547943 | hypothetical protein | - |
Host bacterium
ID | 2013 | GenBank | NZ_JAQMUO010000003 |
Plasmid name | pMFDS1009773 | Incompatibility group | IncFIB |
Plasmid size | 225299 bp | Coordinate of oriT [Strand] | 218225..218348 [-] |
Host baterium | Escherichia coli strain MFDS1009773 |
Cargo genes
Drug resistance gene | - |
Virulence gene | hlyD, hlyB, hlyA, hlyC, estIa, faeJ, faeI, faeH, faeF, faeE, faeD, faeC |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | AcrIF11 |