Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   101569
Name   oriT_pMFDS1009773 in_silico
Organism   Escherichia coli strain MFDS1009773
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JAQMUO010000003 (218225..218348 [-], 124 nt)
oriT length   124 nt
IRs (inverted repeats)      92..99, 113..120  (ATAATGTA..TACATTAT)
 90..95, 107..112  (AAATAA..TTATTT)
 39..46, 49..56  (GCAAAAAC..GTTTTTGC)
 3..10, 15..22  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 124 nt

>oriT_pMFDS1009773
GGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGCTATTTGAATCATTAACTTATGTCTTAAATAATGTATTTTAATTTATTTTACATTATAAAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   1391 GenBank   WP_000986971
Name   traI_V6M11_RS26500_pMFDS1009773 insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 192129.86 Da        Isoelectric Point: 5.8508

>WP_000986971.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Escherichia]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGKLPDGADLSRMQ
DGSNRHRPGYDLTFSAPKSVSMMAMPGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIRAAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAESRMQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTTGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELAMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLISDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKIPVAEGERLRVTGKIPGLRISGGDRLQVASVSEDAMTVVVPGRAEPASLPVGYSPFTALKL
ESGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVVESKNLAFSQVDLLTEAKSFAAEGTSFTDLGREINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETIDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDAIVLVDNVYHRIAGISRDDGLITLQDTDGNSRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVMAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIALGRKYPQPYVALPAFDRNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSEDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAEEKPDQP
DDKTEQAVRDIAGQERDRAVTSEREAVLPESVLREPQRVQDAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

(10-283)

(1434-1557)

(633-712)

(575-626)

(969-1156)


  Protein structure



No available structure.




Auxiliary protein


ID   517 GenBank   WP_332831925
Name   WP_332831925_pMFDS1009773 insolico UniProt ID   _
Length   75 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 75 a.a.        Molecular weight: 9051.13 Da        Isoelectric Point: 9.9851

>WP_332831925.1 conjugal transfer relaxosome protein TraY [Escherichia coli]
MRRRNAKAGTSRTISVYLDEDTNNRLIRAKDRSGRSKTIEIQIRLRDHLKRYPDFYNEEIFREVTEESES
TFKEL

  Protein domains


Predicted by InterproScan.

(14-61)


  Protein structure



No available structure.



ID   518 GenBank   WP_001151555
Name   WP_001151555_pMFDS1009773 insolico UniProt ID   _
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14461.36 Da        Isoelectric Point: 4.5221

>WP_001151555.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Escherichia]
MAKVQAYVSDEIVYKINEIVERRRAEGAKSTDVSFSSISTMLLELGLRVYEAQMERKESAFNQAEFNKVL
LECAIKTQSTVAKILGIESLSPHVSGNPKFEYANMVEDIRDKVSSEMERFFPENDEE

  Protein domains


Predicted by InterproScan.

(1-126)


  Protein structure



No available structure.




T4CP


ID   1029 GenBank   WP_000009375
Name   traD_V6M11_RS26515_pMFDS1009773 insolico UniProt ID   _
Length   714 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 714 a.a.        Molecular weight: 81137.72 Da        Isoelectric Point: 5.1669

>WP_000009375.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Escherichia]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKLSWQTFVNGCIYWWCTTLEGMR
DLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATIVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDLNPEMENRLSAVLAAREAEGRQMASLFEPEVVSGEDVTQAEQPQQPVSSVINDK
KSDAGVSVPAGGIAQELKMKPEEEMEQQLPPGISESGEVVDMAVYEAWQREQNPDIQQQMQRREEVNINV
HRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

(32-128)

(173-560)

  Protein structure



No available structure.



ID   1030 GenBank   WP_001064271
Name   traC_V6M11_RS26630_pMFDS1009773 insolico UniProt ID   _
Length   875 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 875 a.a.        Molecular weight: 99434.22 Da        Isoelectric Point: 5.9665

>WP_001064271.1 MULTISPECIES: type IV secretion system protein TraC [Escherichia]
MNNPLEAVTQAVNSLVTALKLPDESSKANEILGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANETIVEALDHMLRTKLPRGIPLCIHLMSSQLVGERIKYGLREFSWSGEQAERFNAITRAYYMKAAE
TLFPLPEGLNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGAYITTQTVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLVKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIVLLDQYT
ANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWR
LLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRQEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

(289-446)

(468-771)

(38-276)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 189893..218920

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
V6M11_RS26505 189259..189486 + 228 WP_000450526 toxin-antitoxin system antitoxin VapB -
V6M11_RS26510 189486..189884 + 399 WP_000911316 type II toxin-antitoxin system VapC family toxin -
V6M11_RS26515 189893..192037 - 2145 WP_000009375 type IV conjugative transfer system coupling protein TraD virb4
V6M11_RS26520 192093..192823 - 731 Protein_212 hypothetical protein -
V6M11_RS26525 193058..193537 - 480 WP_001355324 hypothetical protein -
V6M11_RS26530 193741..194472 - 732 WP_001364068 conjugal transfer complement resistance protein TraT -
V6M11_RS26535 194504..195007 - 504 WP_000605506 hypothetical protein -
V6M11_RS26540 195023..197866 - 2844 WP_001006995 conjugal transfer mating-pair stabilization protein TraG traG
V6M11_RS26545 197863..199236 - 1374 WP_001137366 conjugal transfer pilus assembly protein TraH traH
V6M11_RS26550 199223..199615 - 393 WP_000138366 F-type conjugal transfer protein TrbF -
V6M11_RS26555 199569..199883 - 315 WP_001244895 P-type conjugative transfer protein TrbJ -
V6M11_RS26560 199873..200409 - 537 WP_000059854 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
V6M11_RS26565 200396..200680 - 285 WP_000624195 type-F conjugative transfer system pilin chaperone TraQ -
V6M11_RS26570 200761..201096 + 336 WP_000415768 hypothetical protein -
V6M11_RS26575 201077..201382 - 306 WP_236922840 conjugal transfer protein TrbA -
V6M11_RS26580 201427..202170 - 744 WP_001030399 type-F conjugative transfer system pilin assembly protein TraF traF
V6M11_RS26585 202163..202420 - 258 WP_000864356 conjugal transfer protein TrbE -
V6M11_RS26590 202434..204338 - 1905 WP_000662057 type-F conjugative transfer system mating-pair stabilization protein TraN traN
V6M11_RS26595 204366..204584 + 219 WP_001364024 hypothetical protein -
V6M11_RS26600 204573..204953 - 381 WP_000009645 hypothetical protein -
V6M11_RS26605 204950..205588 - 639 WP_000087541 type-F conjugative transfer system pilin assembly protein TrbC trbC
V6M11_RS26610 205615..206134 - 520 Protein_230 hypothetical protein -
V6M11_RS26615 206410..207402 - 993 WP_001355328 conjugal transfer pilus assembly protein TraU traU
V6M11_RS26620 207399..208031 - 633 WP_000650119 type-F conjugative transfer system protein TraW traW
V6M11_RS26625 208028..208354 - 327 WP_332831924 type-F conjugative transfer system protein TrbI -
V6M11_RS26630 208412..211039 - 2628 WP_001064271 type IV secretion system protein TraC virb4
V6M11_RS26635 211199..211420 - 222 WP_001278688 conjugal transfer protein TraR -
V6M11_RS26640 211555..212070 - 516 WP_000802746 type IV conjugative transfer system lipoprotein TraV traV
V6M11_RS26645 212067..212387 - 321 WP_001057269 DUF2689 domain-containing protein -
V6M11_RS26650 212374..212961 - 588 WP_000002892 conjugal transfer pilus-stabilizing protein TraP -
V6M11_RS26655 212951..214381 - 1431 WP_000146619 F-type conjugal transfer pilus assembly protein TraB traB
V6M11_RS26660 214381..215109 - 729 WP_001355329 type-F conjugative transfer system secretin TraK traK
V6M11_RS26665 215096..215662 - 567 WP_000399757 type IV conjugative transfer system protein TraE traE
V6M11_RS26670 215684..215995 - 312 WP_000012121 type IV conjugative transfer system protein TraL traL
V6M11_RS26675 216010..216369 - 360 WP_000340277 type IV conjugative transfer system pilin TraA -
V6M11_RS26680 216403..216630 - 228 WP_332831925 conjugal transfer relaxosome protein TraY -
V6M11_RS26685 216724..217410 - 687 WP_000332479 PAS domain-containing protein -
V6M11_RS26690 217604..217987 - 384 WP_001151555 conjugal transfer relaxosome DNA-binding protein TraM -
V6M11_RS26695 218273..218920 + 648 WP_000614283 transglycosylase SLT domain-containing protein virB1
V6M11_RS26700 219217..220038 - 822 Protein_248 DUF932 domain-containing protein -
V6M11_RS26705 220157..220444 - 288 WP_000107535 hypothetical protein -
V6M11_RS26710 220695..221699 + 1005 WP_332831926 IS110 family transposase -
V6M11_RS26715 221793..222053 - 261 WP_000547943 hypothetical protein -


Host bacterium


ID   2013 GenBank   NZ_JAQMUO010000003
Plasmid name   pMFDS1009773 Incompatibility group   IncFIB
Plasmid size   225299 bp Coordinate of oriT [Strand]   218225..218348 [-]
Host baterium   Escherichia coli strain MFDS1009773

Cargo genes


Drug resistance gene   -
Virulence gene   hlyD, hlyB, hlyA, hlyC, estIa, faeJ, faeI, faeH, faeF, faeE, faeD, faeC
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIF11