Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   101562
Name   oriT_pMFDS1015939 in_silico
Organism   Escherichia coli strain MFDS1015939
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JAQMUZ010000002 (203870..203993 [-], 124 nt)
oriT length   124 nt
IRs (inverted repeats)      92..99, 113..120  (ATAATGTA..TACATTAT)
 90..95, 107..112  (AAATAA..TTATTT)
 39..46, 49..56  (GCAAAAAC..GTTTTTGC)
 3..10, 15..22  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 124 nt

>oriT_pMFDS1015939
GGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGCTATTTGAATCATTAACTTATGTCTTAAATAATGTATTTTAATTTATTTTACATTATAAAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   1385 GenBank   WP_000986971
Name   traI_V6M05_RS22370_pMFDS1015939 insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 192129.86 Da        Isoelectric Point: 5.8508

>WP_000986971.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Escherichia]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGKLPDGADLSRMQ
DGSNRHRPGYDLTFSAPKSVSMMAMPGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIRAAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAESRMQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTTGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELAMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLISDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKIPVAEGERLRVTGKIPGLRISGGDRLQVASVSEDAMTVVVPGRAEPASLPVGYSPFTALKL
ESGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVVESKNLAFSQVDLLTEAKSFAAEGTSFTDLGREINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETIDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDAIVLVDNVYHRIAGISRDDGLITLQDTDGNSRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVMAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIALGRKYPQPYVALPAFDRNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSEDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAEEKPDQP
DDKTEQAVRDIAGQERDRAVTSEREAVLPESVLREPQRVQDAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

(10-283)

(1434-1557)

(633-712)

(575-626)

(969-1156)


  Protein structure



No available structure.




Auxiliary protein


ID   506 GenBank   WP_001254381
Name   WP_001254381_pMFDS1015939 insolico UniProt ID   _
Length   75 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 75 a.a.        Molecular weight: 9037.10 Da        Isoelectric Point: 9.9851

>WP_001254381.1 MULTISPECIES: conjugal transfer relaxosome protein TraY [Escherichia]
MRRRNAKAGTSRTISVYLDEDTNNRLIRAKDRSGRSKTIEVQIRLRDHLKRYPDFYNEEIFREVTEESES
TFKEL

  Protein domains


Predicted by InterproScan.

(14-61)


  Protein structure



No available structure.



ID   507 GenBank   WP_001151555
Name   WP_001151555_pMFDS1015939 insolico UniProt ID   _
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14461.36 Da        Isoelectric Point: 4.5221

>WP_001151555.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Escherichia]
MAKVQAYVSDEIVYKINEIVERRRAEGAKSTDVSFSSISTMLLELGLRVYEAQMERKESAFNQAEFNKVL
LECAIKTQSTVAKILGIESLSPHVSGNPKFEYANMVEDIRDKVSSEMERFFPENDEE

  Protein domains


Predicted by InterproScan.

(1-126)


  Protein structure



No available structure.




T4CP


ID   1018 GenBank   WP_000009375
Name   traD_V6M05_RS22385_pMFDS1015939 insolico UniProt ID   _
Length   714 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 714 a.a.        Molecular weight: 81137.72 Da        Isoelectric Point: 5.1669

>WP_000009375.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Escherichia]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKLSWQTFVNGCIYWWCTTLEGMR
DLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATIVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDLNPEMENRLSAVLAAREAEGRQMASLFEPEVVSGEDVTQAEQPQQPVSSVINDK
KSDAGVSVPAGGIAQELKMKPEEEMEQQLPPGISESGEVVDMAVYEAWQREQNPDIQQQMQRREEVNINV
HRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

(32-128)

(173-560)

  Protein structure



No available structure.



ID   1019 GenBank   WP_332829366
Name   traC_V6M05_RS22500_pMFDS1015939 insolico UniProt ID   _
Length   817 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 817 a.a.        Molecular weight: 92622.60 Da        Isoelectric Point: 5.7355

>WP_332829366.1 type IV secretion system protein TraC [Escherichia coli]
MNNPLEAVTQAVNSLVTALKLPDESSKANEILGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANETIVEALDHMLRTKLPRGIPLCIHLMSSQLVGERIKYGLREFSWSGEQAERFNAITRAYYMKAAE
TLFPLPEGLNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGAYITTQTVDAQAFIDIVG
EMINHNPEIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWF
PSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRN
FLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNT
NYNMAVCGTSGAGKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDID
QSAERVRDQLSVMASPNGNLDEVHEGLLLQAVRASWLVKENRARIDDVVDFLKNASDSEQYAESPTIRSR
LDEMIVLLDQYTANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRN
LKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKII
LKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPD
FEFVQQKRQEGLSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

(38-219)

(231-388)

(409-713)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 175713..204565

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
V6M05_RS22375 175079..175306 + 228 WP_000450526 toxin-antitoxin system antitoxin VapB -
V6M05_RS22380 175306..175704 + 399 WP_000911316 type II toxin-antitoxin system VapC family toxin -
V6M05_RS22385 175713..177857 - 2145 WP_000009375 type IV conjugative transfer system coupling protein TraD virb4
V6M05_RS22390 177913..178643 - 731 Protein_187 hypothetical protein -
V6M05_RS22395 178878..179357 - 480 WP_001355324 hypothetical protein -
V6M05_RS22400 179561..180292 - 732 WP_001364068 conjugal transfer complement resistance protein TraT -
V6M05_RS22405 180324..180827 - 504 WP_000605506 hypothetical protein -
V6M05_RS22410 180843..183686 - 2844 WP_001006995 conjugal transfer mating-pair stabilization protein TraG traG
V6M05_RS22415 183683..185056 - 1374 WP_001137366 conjugal transfer pilus assembly protein TraH traH
V6M05_RS22420 185043..185435 - 393 WP_000138366 F-type conjugal transfer protein TrbF -
V6M05_RS22425 185389..185703 - 315 WP_001244895 P-type conjugative transfer protein TrbJ -
V6M05_RS22430 185693..186229 - 537 WP_000059854 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
V6M05_RS22435 186216..186500 - 285 WP_000624195 type-F conjugative transfer system pilin chaperone TraQ -
V6M05_RS22440 186581..186916 + 336 WP_000415768 hypothetical protein -
V6M05_RS22445 186897..187202 - 306 WP_236922840 conjugal transfer protein TrbA -
V6M05_RS22450 187247..187990 - 744 WP_001030399 type-F conjugative transfer system pilin assembly protein TraF traF
V6M05_RS22455 187983..188240 - 258 WP_000864356 conjugal transfer protein TrbE -
V6M05_RS22460 188254..190158 - 1905 WP_000662057 type-F conjugative transfer system mating-pair stabilization protein TraN traN
V6M05_RS22465 190186..190404 + 219 WP_001364024 hypothetical protein -
V6M05_RS22470 190393..190773 - 381 WP_000009645 hypothetical protein -
V6M05_RS22475 190770..191408 - 639 WP_000087541 type-F conjugative transfer system pilin assembly protein TrbC trbC
V6M05_RS22480 191435..191954 - 520 Protein_205 hypothetical protein -
V6M05_RS22485 192230..193222 - 993 WP_001355328 conjugal transfer pilus assembly protein TraU traU
V6M05_RS22490 193219..193851 - 633 WP_000650119 type-F conjugative transfer system protein TraW traW
V6M05_RS22495 193848..194234 - 387 WP_000214089 type-F conjugative transfer system protein TrbI -
V6M05_RS22500 194231..196684 - 2454 WP_332829366 type IV secretion system protein TraC virb4
V6M05_RS22505 196844..197065 - 222 WP_001278688 conjugal transfer protein TraR -
V6M05_RS22510 197200..197715 - 516 WP_000802746 type IV conjugative transfer system lipoprotein TraV traV
V6M05_RS22515 197712..198032 - 321 WP_001057269 DUF2689 domain-containing protein -
V6M05_RS22520 198019..198606 - 588 WP_000002892 conjugal transfer pilus-stabilizing protein TraP -
V6M05_RS22525 198596..200026 - 1431 WP_000146619 F-type conjugal transfer pilus assembly protein TraB traB
V6M05_RS22530 200026..200754 - 729 WP_001355329 type-F conjugative transfer system secretin TraK traK
V6M05_RS22535 200741..201307 - 567 WP_000399757 type IV conjugative transfer system protein TraE traE
V6M05_RS22540 201329..201640 - 312 WP_000012121 type IV conjugative transfer system protein TraL traL
V6M05_RS22545 201655..202014 - 360 WP_000340277 type IV conjugative transfer system pilin TraA -
V6M05_RS22550 202048..202275 - 228 WP_001254381 conjugal transfer relaxosome protein TraY -
V6M05_RS22555 202369..203055 - 687 WP_000332479 PAS domain-containing protein -
V6M05_RS22560 203249..203632 - 384 WP_001151555 conjugal transfer relaxosome DNA-binding protein TraM -
V6M05_RS22565 203918..204565 + 648 WP_000614283 transglycosylase SLT domain-containing protein virB1
V6M05_RS22570 204862..205683 - 822 Protein_223 DUF932 domain-containing protein -
V6M05_RS22575 205802..206089 - 288 WP_000107535 hypothetical protein -
V6M05_RS22580 206340..207344 + 1005 WP_024166184 IS110 family transposase -
V6M05_RS22585 207438..207698 - 261 WP_000547943 hypothetical protein -
V6M05_RS22590 207768..207940 + 173 Protein_227 hypothetical protein -


Host bacterium


ID   2006 GenBank   NZ_JAQMUZ010000002
Plasmid name   pMFDS1015939 Incompatibility group   IncFIB
Plasmid size   226505 bp Coordinate of oriT [Strand]   203870..203993 [-]
Host baterium   Escherichia coli strain MFDS1015939

Cargo genes


Drug resistance gene   -
Virulence gene   hlyD, hlyB, hlyA, hlyC, estIa, faeJ, faeI, faeH, faeF, faeE, faeD, faeC
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -