Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 101562 |
Name | oriT_pMFDS1015939 |
Organism | Escherichia coli strain MFDS1015939 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_JAQMUZ010000002 (203870..203993 [-], 124 nt) |
oriT length | 124 nt |
IRs (inverted repeats) | 92..99, 113..120 (ATAATGTA..TACATTAT) 90..95, 107..112 (AAATAA..TTATTT) 39..46, 49..56 (GCAAAAAC..GTTTTTGC) 3..10, 15..22 (TTGGTGGT..ACCACCAA) |
Location of nic site | _ |
Conserved sequence flanking the nic site |
_ |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 124 nt
GGTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGCTATTTGAATCATTAACTTATGTCTTAAATAATGTATTTTAATTTATTTTACATTATAAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 1385 | GenBank | WP_000986971 |
Name | traI_V6M05_RS22370_pMFDS1015939 | UniProt ID | _ |
Length | 1756 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 1756 a.a. Molecular weight: 192129.86 Da Isoelectric Point: 5.8508
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGKLPDGADLSRMQ
DGSNRHRPGYDLTFSAPKSVSMMAMPGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIRAAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAADQRAESRMQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTTGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELAMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLISDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKIPVAEGERLRVTGKIPGLRISGGDRLQVASVSEDAMTVVVPGRAEPASLPVGYSPFTALKL
ESGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVVESKNLAFSQVDLLTEAKSFAAEGTSFTDLGREINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETIDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDAIVLVDNVYHRIAGISRDDGLITLQDTDGNSRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVMAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIALGRKYPQPYVALPAFDRNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSEDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAEEKPDQP
DDKTEQAVRDIAGQERDRAVTSEREAVLPESVLREPQRVQDAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
ID | 506 | GenBank | WP_001254381 |
Name | WP_001254381_pMFDS1015939 | UniProt ID | _ |
Length | 75 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 75 a.a. Molecular weight: 9037.10 Da Isoelectric Point: 9.9851
MRRRNAKAGTSRTISVYLDEDTNNRLIRAKDRSGRSKTIEVQIRLRDHLKRYPDFYNEEIFREVTEESES
TFKEL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
ID | 507 | GenBank | WP_001151555 |
Name | WP_001151555_pMFDS1015939 | UniProt ID | _ |
Length | 127 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 127 a.a. Molecular weight: 14461.36 Da Isoelectric Point: 4.5221
MAKVQAYVSDEIVYKINEIVERRRAEGAKSTDVSFSSISTMLLELGLRVYEAQMERKESAFNQAEFNKVL
LECAIKTQSTVAKILGIESLSPHVSGNPKFEYANMVEDIRDKVSSEMERFFPENDEE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
ID | 1018 | GenBank | WP_000009375 |
Name | traD_V6M05_RS22385_pMFDS1015939 | UniProt ID | _ |
Length | 714 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 714 a.a. Molecular weight: 81137.72 Da Isoelectric Point: 5.1669
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKLSWQTFVNGCIYWWCTTLEGMR
DLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATIVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDLNPEMENRLSAVLAAREAEGRQMASLFEPEVVSGEDVTQAEQPQQPVSSVINDK
KSDAGVSVPAGGIAQELKMKPEEEMEQQLPPGISESGEVVDMAVYEAWQREQNPDIQQQMQRREEVNINV
HRERGEDVEPGDDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
ID | 1019 | GenBank | WP_332829366 |
Name | traC_V6M05_RS22500_pMFDS1015939 | UniProt ID | _ |
Length | 817 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 817 a.a. Molecular weight: 92622.60 Da Isoelectric Point: 5.7355
MNNPLEAVTQAVNSLVTALKLPDESSKANEILGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANETIVEALDHMLRTKLPRGIPLCIHLMSSQLVGERIKYGLREFSWSGEQAERFNAITRAYYMKAAE
TLFPLPEGLNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGAYITTQTVDAQAFIDIVG
EMINHNPEIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWF
PSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRN
FLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNT
NYNMAVCGTSGAGKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDID
QSAERVRDQLSVMASPNGNLDEVHEGLLLQAVRASWLVKENRARIDDVVDFLKNASDSEQYAESPTIRSR
LDEMIVLLDQYTANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRN
LKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKII
LKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPD
FEFVQQKRQEGLSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 175713..204565
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
V6M05_RS22375 | 175079..175306 | + | 228 | WP_000450526 | toxin-antitoxin system antitoxin VapB | - |
V6M05_RS22380 | 175306..175704 | + | 399 | WP_000911316 | type II toxin-antitoxin system VapC family toxin | - |
V6M05_RS22385 | 175713..177857 | - | 2145 | WP_000009375 | type IV conjugative transfer system coupling protein TraD | virb4 |
V6M05_RS22390 | 177913..178643 | - | 731 | Protein_187 | hypothetical protein | - |
V6M05_RS22395 | 178878..179357 | - | 480 | WP_001355324 | hypothetical protein | - |
V6M05_RS22400 | 179561..180292 | - | 732 | WP_001364068 | conjugal transfer complement resistance protein TraT | - |
V6M05_RS22405 | 180324..180827 | - | 504 | WP_000605506 | hypothetical protein | - |
V6M05_RS22410 | 180843..183686 | - | 2844 | WP_001006995 | conjugal transfer mating-pair stabilization protein TraG | traG |
V6M05_RS22415 | 183683..185056 | - | 1374 | WP_001137366 | conjugal transfer pilus assembly protein TraH | traH |
V6M05_RS22420 | 185043..185435 | - | 393 | WP_000138366 | F-type conjugal transfer protein TrbF | - |
V6M05_RS22425 | 185389..185703 | - | 315 | WP_001244895 | P-type conjugative transfer protein TrbJ | - |
V6M05_RS22430 | 185693..186229 | - | 537 | WP_000059854 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
V6M05_RS22435 | 186216..186500 | - | 285 | WP_000624195 | type-F conjugative transfer system pilin chaperone TraQ | - |
V6M05_RS22440 | 186581..186916 | + | 336 | WP_000415768 | hypothetical protein | - |
V6M05_RS22445 | 186897..187202 | - | 306 | WP_236922840 | conjugal transfer protein TrbA | - |
V6M05_RS22450 | 187247..187990 | - | 744 | WP_001030399 | type-F conjugative transfer system pilin assembly protein TraF | traF |
V6M05_RS22455 | 187983..188240 | - | 258 | WP_000864356 | conjugal transfer protein TrbE | - |
V6M05_RS22460 | 188254..190158 | - | 1905 | WP_000662057 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
V6M05_RS22465 | 190186..190404 | + | 219 | WP_001364024 | hypothetical protein | - |
V6M05_RS22470 | 190393..190773 | - | 381 | WP_000009645 | hypothetical protein | - |
V6M05_RS22475 | 190770..191408 | - | 639 | WP_000087541 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
V6M05_RS22480 | 191435..191954 | - | 520 | Protein_205 | hypothetical protein | - |
V6M05_RS22485 | 192230..193222 | - | 993 | WP_001355328 | conjugal transfer pilus assembly protein TraU | traU |
V6M05_RS22490 | 193219..193851 | - | 633 | WP_000650119 | type-F conjugative transfer system protein TraW | traW |
V6M05_RS22495 | 193848..194234 | - | 387 | WP_000214089 | type-F conjugative transfer system protein TrbI | - |
V6M05_RS22500 | 194231..196684 | - | 2454 | WP_332829366 | type IV secretion system protein TraC | virb4 |
V6M05_RS22505 | 196844..197065 | - | 222 | WP_001278688 | conjugal transfer protein TraR | - |
V6M05_RS22510 | 197200..197715 | - | 516 | WP_000802746 | type IV conjugative transfer system lipoprotein TraV | traV |
V6M05_RS22515 | 197712..198032 | - | 321 | WP_001057269 | DUF2689 domain-containing protein | - |
V6M05_RS22520 | 198019..198606 | - | 588 | WP_000002892 | conjugal transfer pilus-stabilizing protein TraP | - |
V6M05_RS22525 | 198596..200026 | - | 1431 | WP_000146619 | F-type conjugal transfer pilus assembly protein TraB | traB |
V6M05_RS22530 | 200026..200754 | - | 729 | WP_001355329 | type-F conjugative transfer system secretin TraK | traK |
V6M05_RS22535 | 200741..201307 | - | 567 | WP_000399757 | type IV conjugative transfer system protein TraE | traE |
V6M05_RS22540 | 201329..201640 | - | 312 | WP_000012121 | type IV conjugative transfer system protein TraL | traL |
V6M05_RS22545 | 201655..202014 | - | 360 | WP_000340277 | type IV conjugative transfer system pilin TraA | - |
V6M05_RS22550 | 202048..202275 | - | 228 | WP_001254381 | conjugal transfer relaxosome protein TraY | - |
V6M05_RS22555 | 202369..203055 | - | 687 | WP_000332479 | PAS domain-containing protein | - |
V6M05_RS22560 | 203249..203632 | - | 384 | WP_001151555 | conjugal transfer relaxosome DNA-binding protein TraM | - |
V6M05_RS22565 | 203918..204565 | + | 648 | WP_000614283 | transglycosylase SLT domain-containing protein | virB1 |
V6M05_RS22570 | 204862..205683 | - | 822 | Protein_223 | DUF932 domain-containing protein | - |
V6M05_RS22575 | 205802..206089 | - | 288 | WP_000107535 | hypothetical protein | - |
V6M05_RS22580 | 206340..207344 | + | 1005 | WP_024166184 | IS110 family transposase | - |
V6M05_RS22585 | 207438..207698 | - | 261 | WP_000547943 | hypothetical protein | - |
V6M05_RS22590 | 207768..207940 | + | 173 | Protein_227 | hypothetical protein | - |
Host bacterium
ID | 2006 | GenBank | NZ_JAQMUZ010000002 |
Plasmid name | pMFDS1015939 | Incompatibility group | IncFIB |
Plasmid size | 226505 bp | Coordinate of oriT [Strand] | 203870..203993 [-] |
Host baterium | Escherichia coli strain MFDS1015939 |
Cargo genes
Drug resistance gene | - |
Virulence gene | hlyD, hlyB, hlyA, hlyC, estIa, faeJ, faeI, faeH, faeF, faeE, faeD, faeC |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |