Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101554 |
| Name | oriT_pMFDS1007784  |
| Organism | Escherichia coli strain MFDS1007784 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JAQJCX010000002 (77985..78070 [+], 86 nt) |
| oriT length | 86 nt |
| IRs (inverted repeats) | 27..34, 37..44 (GCAAAAAC..GTTTTTGC) |
| Location of nic site | 53..54 |
| Conserved sequence flanking the nic site |
GGTGTGGTGC |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 86 nt
>oriT_pMFDS1007784
AACTACTTATTTGCAAAGAAAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGCGGTGAGAACCACCAACCTGTT
AACTACTTATTTGCAAAGAAAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGCGGTGAGAACCACCAACCTGTT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1377 | GenBank | WP_052934445 |
| Name | traI_PI248_RS26070_pMFDS1007784 |
UniProt ID | _ |
| Length | 1756 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1756 a.a. Molecular weight: 191804.37 Da Isoelectric Point: 5.7425
>WP_052934445.1 conjugative transfer relaxase/helicase TraI [Escherichia coli]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGKLPDGADLSRMQ
DGSNRHRPGYDLTFSAPKSVSMMAMPGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAVDQRAESRMQTPGPASQDGPDVQQAVTQAIAGLSERNVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLISLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTTGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELAMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLISDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKIPVAEGERLRVTGKIPGLRISGGDRLQVASVSEDAMTVVVPGRAEPASLPVGYSPFTALKL
ESGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVVESKNLAFSQVDLLTEAKSFAAEGTSFTDLGREINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETIDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALVLVDNVYHRIAGISKDDGLITLQDTDGNSRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYGANSVWTVMAVSGDSVTLSDGQQTRVIRPGQEQAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGGRKQMAGFESAYVALSRMKQHVQVYTDNCQGWTDAINNAVQKGTAHDVL
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPGNSVQPGGGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAEEKPDQP
DDKTEQAVRDIAGQERDRAVTSEREAVLPESVLREPQRVQDAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGKLPDGADLSRMQ
DGSNRHRPGYDLTFSAPKSVSMMAMPGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQT
LKETGFDIRAYRDAVDQRAESRMQTPGPASQDGPDVQQAVTQAIAGLSERNVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLISLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTTGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELAMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLISDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKIPVAEGERLRVTGKIPGLRISGGDRLQVASVSEDAMTVVVPGRAEPASLPVGYSPFTALKL
ESGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVVESKNLAFSQVDLLTEAKSFAAEGTSFTDLGREINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETIDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALVLVDNVYHRIAGISKDDGLITLQDTDGNSRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYGANSVWTVMAVSGDSVTLSDGQQTRVIRPGQEQAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGGRKQMAGFESAYVALSRMKQHVQVYTDNCQGWTDAINNAVQKGTAHDVL
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPGNSVQPGGGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAEEKPDQP
DDKTEQAVRDIAGQERDRAVTSEREAVLPESVLREPQRVQDAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 497 | GenBank | WP_053265379 |
| Name | WP_053265379_pMFDS1007784 |
UniProt ID | _ |
| Length | 124 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 124 a.a. Molecular weight: 14464.67 Da Isoelectric Point: 10.2984
>WP_053265379.1 conjugal transfer relaxosome DNA-bindin protein TraY [Escherichia coli]
MKRFGTRSATGKMVKLKLPVDVEALLIEASERSGRSRSFEAVIRLKDHLHRYPKFNRISNVYGKSLVKYL
TMRLDDETNKLLIAAKNRSGWCKTDEAADRVIDHLIKFPDFYNSEIYREATEQR
MKRFGTRSATGKMVKLKLPVDVEALLIEASERSGRSRSFEAVIRLKDHLHRYPKFNRISNVYGKSLVKYL
TMRLDDETNKLLIAAKNRSGWCKTDEAADRVIDHLIKFPDFYNSEIYREATEQR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 498 | GenBank | WP_069894497 |
| Name | WP_069894497_pMFDS1007784 |
UniProt ID | _ |
| Length | 127 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 127 a.a. Molecular weight: 14446.44 Da Isoelectric Point: 5.0286
>WP_069894497.1 conjugal transfer relaxosome DNA-binding protein TraM [Escherichia coli]
MAKIQVYVNDHVSEKINAIAVQRRAEGAKEKDVSYSSIASMLLELGLRVYEAQMERKETAFNQTEFNKVL
LECVVKTQSSVAKILGIESLSPHVAGNSKFEYANMVEDIRDKVSVEMERFFPRNDEE
MAKIQVYVNDHVSEKINAIAVQRRAEGAKEKDVSYSSIASMLLELGLRVYEAQMERKETAFNQTEFNKVL
LECVVKTQSSVAKILGIESLSPHVAGNSKFEYANMVEDIRDKVSVEMERFFPRNDEE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 1010 | GenBank | WP_332391478 |
| Name | traD_PI248_RS26085_pMFDS1007784 |
UniProt ID | _ |
| Length | 744 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 744 a.a. Molecular weight: 84719.52 Da Isoelectric Point: 5.1066
>WP_332391478.1 type IV conjugative transfer system coupling protein TraD [Escherichia coli]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKLSWQTFMNGCIYWWCTTLEGMR
DLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGYLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPREINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVVQAEQPQQPQQPQQ
PQQPQQPQQPQQPQQPQQPQQPVSSVINDKKSDAGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVV
DMAAYEAWQQENHPDTQQQMQRREEVNINVHRERGEDVEPGDDF
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKLSWQTFMNGCIYWWCTTLEGMR
DLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGYLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPREINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVVQAEQPQQPQQPQQ
PQQPQQPQQPQQPQQPQQPQQPVSSVINDKKSDAGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVV
DMAAYEAWQQENHPDTQQQMQRREEVNINVHRERGEDVEPGDDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 1011 | GenBank | WP_069894487 |
| Name | traC_PI248_RS26195_pMFDS1007784 |
UniProt ID | _ |
| Length | 875 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 875 a.a. Molecular weight: 99339.99 Da Isoelectric Point: 5.8499
>WP_069894487.1 type IV secretion system protein TraC [Escherichia coli]
MNNPLEAVTQAVNSLLTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAA
TQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGDSITTQTVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIVLLDQYT
ANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNFKKLNVIDEGWR
LLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA
MNNPLEAVTQAVNSLLTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAIP
INGANESIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAA
TQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGDSITTQTVDAQAFIDIVG
EMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFL
WNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWGE
LRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKG
LFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGA
GKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSV
MASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIVLLDQYT
ANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNFKKLNVIDEGWR
LLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYN
QLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGL
SIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 48986..78638
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PI248_RS26075 | 48352..48579 | + | 228 | WP_000450526 | toxin-antitoxin system antitoxin VapB | - |
| PI248_RS26080 | 48579..48977 | + | 399 | WP_000911317 | type II toxin-antitoxin system VapC family toxin | - |
| PI248_RS26085 | 48986..51220 | - | 2235 | WP_332391478 | type IV conjugative transfer system coupling protein TraD | virb4 |
| PI248_RS26090 | 51276..52006 | - | 731 | Protein_55 | hypothetical protein | - |
| PI248_RS26095 | 52241..52720 | - | 480 | WP_097467486 | hypothetical protein | - |
| PI248_RS26100 | 52923..53654 | - | 732 | WP_123008208 | conjugal transfer complement resistance protein TraT | - |
| PI248_RS26105 | 53703..54182 | - | 480 | WP_137545333 | surface exclusion protein | - |
| PI248_RS26110 | 54204..57041 | - | 2838 | WP_137545332 | conjugal transfer mating-pair stabilization protein TraG | traG |
| PI248_RS26115 | 57038..58411 | - | 1374 | WP_097467439 | conjugal transfer pilus assembly protein TraH | traH |
| PI248_RS26120 | 58398..58790 | - | 393 | WP_244584896 | F-type conjugal transfer protein TrbF | - |
| PI248_RS26125 | 58744..59058 | - | 315 | WP_244584892 | P-type conjugative transfer protein TrbJ | - |
| PI248_RS26130 | 59048..59593 | - | 546 | WP_097446509 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| PI248_RS26135 | 59580..59870 | - | 291 | WP_097446493 | type-F conjugative transfer system pilin chaperone TraQ | - |
| PI248_RS26140 | 60268..60612 | - | 345 | WP_097446491 | conjugal transfer protein TrbA | - |
| PI248_RS26145 | 60744..61109 | - | 366 | WP_097446490 | hypothetical protein | - |
| PI248_RS26150 | 61123..61866 | - | 744 | WP_001030392 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| PI248_RS26155 | 61859..62116 | - | 258 | WP_000864351 | conjugal transfer protein TrbE | - |
| PI248_RS26160 | 62130..64028 | - | 1899 | WP_069894479 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| PI248_RS26165 | 64122..64502 | - | 381 | WP_032206670 | hypothetical protein | - |
| PI248_RS26170 | 64499..65138 | - | 640 | Protein_71 | type-F conjugative transfer system pilin assembly protein TrbC | - |
| PI248_RS26175 | 65367..65699 | - | 333 | WP_069894483 | hypothetical protein | - |
| PI248_RS26180 | 65726..66718 | - | 993 | WP_065225974 | conjugal transfer pilus assembly protein TraU | traU |
| PI248_RS26185 | 66715..67347 | - | 633 | WP_069894485 | type-F conjugative transfer system protein TraW | traW |
| PI248_RS26190 | 67344..67730 | - | 387 | WP_047088907 | type-F conjugative transfer system protein TrbI | - |
| PI248_RS26195 | 67727..70354 | - | 2628 | WP_069894487 | type IV secretion system protein TraC | virb4 |
| PI248_RS26200 | 70514..70735 | - | 222 | WP_042966711 | conjugal transfer protein TraR | - |
| PI248_RS26205 | 70870..71385 | - | 516 | WP_042966709 | type IV conjugative transfer system lipoprotein TraV | traV |
| PI248_RS26210 | 71382..71633 | - | 252 | WP_042966708 | conjugal transfer protein TrbG | - |
| PI248_RS26215 | 71626..71946 | - | 321 | WP_042966707 | conjugal transfer protein TrbD | virb4 |
| PI248_RS26220 | 71933..72520 | - | 588 | WP_069894489 | conjugal transfer pilus-stabilizing protein TraP | - |
| PI248_RS26225 | 72510..73928 | - | 1419 | WP_069894491 | F-type conjugal transfer pilus assembly protein TraB | traB |
| PI248_RS26230 | 73928..74656 | - | 729 | WP_001355329 | type-F conjugative transfer system secretin TraK | traK |
| PI248_RS26235 | 74643..75209 | - | 567 | WP_000399757 | type IV conjugative transfer system protein TraE | traE |
| PI248_RS26240 | 75231..75542 | - | 312 | WP_000012108 | type IV conjugative transfer system protein TraL | traL |
| PI248_RS26245 | 75557..75910 | - | 354 | WP_069894493 | type IV conjugative transfer system pilin TraA | - |
| PI248_RS26250 | 75966..76340 | - | 375 | WP_053265379 | conjugal transfer relaxosome DNA-bindin protein TraY | - |
| PI248_RS26255 | 76439..77128 | - | 690 | WP_069894495 | conjugal transfer transcriptional regulator TraJ | - |
| PI248_RS26260 | 77313..77696 | - | 384 | WP_069894497 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| PI248_RS26265 | 78036..78638 | + | 603 | WP_077888346 | transglycosylase SLT domain-containing protein | virB1 |
| PI248_RS26270 | 78935..79756 | - | 822 | WP_137461907 | DUF932 domain-containing protein | - |
| PI248_RS26275 | 79874..80163 | - | 290 | Protein_92 | hypothetical protein | - |
| PI248_RS26280 | 80414..81418 | + | 1005 | WP_069894512 | IS110 family transposase | - |
| PI248_RS26285 | 81859..82014 | + | 156 | Protein_94 | hypothetical protein | - |
Host bacterium
| ID | 1998 | GenBank | NZ_JAQJCX010000002 |
| Plasmid name | pMFDS1007784 | Incompatibility group | IncFIB |
| Plasmid size | 153254 bp | Coordinate of oriT [Strand] | 77985..78070 [+] |
| Host baterium | Escherichia coli strain MFDS1007784 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | estIa |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |