Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 101552
• Name: oriT_pL1M1-01d
• Organism: Escherichia coli strain L1M1-01
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_JAPZDR010000004 (977..1036 [+], 60 nt)
• oriT length: 60 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 60 nt

>oriT_pL1M1-01d
GGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_O8D02_RS24595_pL1M1-01d

• ID: 1375
• GenBank: WP_327643590
• Name: Relaxase_O8D02_RS24595_pL1M1-01d
• UniProt ID: _
• Length: 185 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 185 a.a.
• Molecular weight: 20541.51 Da
• Isoelectric Point: 9.5883

>WP_327643590.1 relaxase/mobilization nuclease domain-containing protein, partial [Escherichia coli]
MIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELIDASPYAKKYTSGVLSFAEAELPPGQR
EQIMASFERVLMPGLDKDQYSILWVEHTDKGRLELNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTVVN
GRLGLHDPNAPKNRRLLVTPSALPETKLEAAQAITRGLLALASSG

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (55-164)

Protein structure

No available structure.

Auxiliary protein

WP_000957082_pL1M1-01d

• ID: 494
• GenBank: WP_000957082
• Name: WP_000957082_pL1M1-01d
• UniProt ID: _
• Length: 106 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 106 a.a.
• Molecular weight: 11739.54 Da
• Isoelectric Point: 9.4905

>WP_000957082.1 MULTISPECIES: MobC family plasmid mobilization relaxosome protein [Enterobacteriaceae]
MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSISPALLRQLAGMGNNLNQIARKVNTG
GAGHDRVQIVAALMAIDAGLERLRHAVLEKGGNDDR

Protein domains

Predicted by InterproScan.

• Bacterial mobilisation protein (MobC) (MobC) (50-93)

Protein structure

No available structure.

WP_001749519_pL1M1-01d

• ID: 495
• GenBank: WP_001749519
• Name: WP_001749519_pL1M1-01d
• UniProt ID: A0A6Y5XIL9
• Length: 161 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 161 a.a.
• Molecular weight: 17752.43 Da
• Isoelectric Point: 9.6101

>WP_001749519.1 MULTISPECIES: MbeB family mobilization protein [Enterobacteriaceae]
MSSLLALAKDLEKQSKAQQQNTCEMLKAAFSEHEKSVKAELSASAKRISDAISAHEQGMTAAMQSNRLSV
LRMVGRTWLTITLVSVLLIATSGSILWWQGQQITGNYQTIRAQERTQAMLSEKNHGVQLSLCGEQKLSCV
KVNPKAGAYGEEGNWMVLERK

Protein domains

Predicted by InterproScan.

• MbeB-like, N-term conserved region (MbeB_N) (1-52)

Protein structure

• AlphaFold DB: A0A6Y5XIL9

Host bacterium

• ID: 1996
• GenBank: NZ_JAPZDR010000004
• Plasmid name: pL1M1-01d
• Incompatibility group: Col440II
• Plasmid size: 5429 bp
• Coordinate of oriT [Strand]: 977..1036 [+]
• Host baterium: Escherichia coli strain L1M1-01

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -