Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 101544
• Name: oriT1_FWSEC0398|unnamed6
• Organism: Escherichia coli strain FWSEC0398
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_RRNV01000100 (22642..22727 [-], 86 nt)
• oriT length: 86 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 86 nt

>oriT1_FWSEC0398|unnamed6
CGGGGTGTCGGGGCTAAGCCCTGACCAGGTGGTAATCGTATAGCCGTGCGTGCGCGGTTATACGATTACACATCCTGTCCCGTTTT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_C9173_RS23595_FWSEC0398|unnamed6

• ID: 1369
• GenBank: WP_136748012
• Name: Relaxase_C9173_RS23595_FWSEC0398|unnamed6
• UniProt ID: _
• Length: 906 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 906 a.a.
• Molecular weight: 104931.66 Da
• Isoelectric Point: 8.1134

>WP_136748012.1 relaxase/mobilization nuclease domain-containing protein [Escherichia coli]
MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEIPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCLSLESAAEEMQKAAELAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIDQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDKAREGIALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRHFAERLPAMARNGELKSCLAVHRILAEAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVVPGERFRGHSIGSQPVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
GLIQCRSLIEEMIADGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRECREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRTTNVDRCVVLCEPGGTPLFNNVAKLEARLQK
NGSVHFRDTRTGKNVCTDYGDRVVFYHHTDRNELAEKLNLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM
VAWHNAAGITGNGHFTITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWEPPSPGM

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (62-315)

Protein structure

No available structure.

Auxiliary protein

WP_001291057_FWSEC0398|unnamed6

• ID: 483
• GenBank: WP_001291057
• Name: WP_001291057_FWSEC0398|unnamed6
• UniProt ID: A0A142CND4
• Length: 110 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 110 a.a.
• Molecular weight: 12596.69 Da
• Isoelectric Point: 10.7335

>WP_001291057.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVSTLIRKTVLGKQINARIDEDFLKELMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITELANTLRRNLMGR

Protein domains

Predicted by InterproScan.

• Family of unknown function (DUF6290) (DUF6290) (19-66)

Protein structure

• AlphaFold DB: A0A142CND4

T4CP

t4cp2_C9173_RS24440_FWSEC0398|unnamed6

• ID: 997
• GenBank: WP_162534295
• Name: t4cp2_C9173_RS24440_FWSEC0398|unnamed6
• UniProt ID: _
• Length: 489 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 489 a.a.
• Molecular weight: 55147.40 Da
• Isoelectric Point: 5.5566

>WP_162534295.1 TraM recognition domain-containing protein [Escherichia coli]
MSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQ
FSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSALGRMFVTQQSMILARDLGY
RLEGTDAQPLEVKKYKGSFPYICILDEVGAYYTDRIAVEATQVRSLEFSLIMMAQDQERIEGQTSATNTA
TLMQNAGTKFAGRIVSDDKTARTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLI
RLNAGEFFTVFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPEHVS
SIIGVLTAKPSRKRRKNRTEPYRIIDTFQRRLATAQTSLDLLPQYDTDIESRANELWKKAVHTINNTTRE
ERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEILLPVAENNKSKTINNDHPQKSCGAQPTTDEKY

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (162-251)

Protein structure

No available structure.

t4cp1_C9173_RS24515_FWSEC0398|unnamed6

• ID: 998
• GenBank: WP_274536677
• Name: t4cp1_C9173_RS24515_FWSEC0398|unnamed6
• UniProt ID: _
• Length: 428 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 428 a.a.
• Molecular weight: 49200.79 Da
• Isoelectric Point: 10.4408

>WP_274536677.1 hypothetical protein [Escherichia coli]
MSYQHSPTTEDPMNIWYIVGIICLLFALLIWLFLPDIVFATCLILHTMWGMTDWGPFHHFAAPRYNLLAN
TANNAATITFSQWLDVMSQTVRILWLILLPMTIGFLWMWFQHPAQPRFTRRPLNIHTLPHLFSSLSPAIA
PVLADGDSNRLFHGQKRPERRVALTPEVFVAQNNLIRNMQLDVAATRQRFMAQLGQPLTTWKDMAPHEKA
LFAVFGLQFFLGDRKAAVSLMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQQWLRQHRYR
RINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNPLCWGRGFTFVDGKAQNDTTRTIWYLSRRF
GREDDIEVINFMNGGKSRSEVIQSGDKSRPQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAM
NKALVFGT

Protein domains

No domain identified.

Protein structure

No available structure.

Host bacterium

• ID: 1988
• GenBank: NZ_RRNV01000100
• Plasmid name: FWSEC0398|unnamed6
• Incompatibility group: -
• Plasmid size: 42320 bp
• Coordinate of oriT [Strand]: 22642..22727 [-]; 39924..40181 [+]
• Host baterium: Escherichia coli strain FWSEC0398

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -