Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101542
Name   oriT_FWSEC0398|unnamed3 in_silico
Organism   Escherichia coli strain FWSEC0398
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_RRNV01000097 (23801..23903 [-], 103 nt)
oriT length   103 nt
IRs (inverted repeats)      65..70, 73..78  (CACCGT..ACGGTG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 103 nt

>oriT_FWSEC0398|unnamed3
AGCCTCGCAGAGCAGGACTCCCGTTGAGCAGCCCCGCTGCGAATAAGGGAAGGTAAAGTAGAAGCACCGTTTACGGTGGGCCTACTTTACATGTCCTGCCCGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1367 GenBank   WP_169073250
Name   Relaxase_C9173_RS23270_FWSEC0398|unnamed3 insolico UniProt ID   _
Length   877 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 877 a.a.        Molecular weight: 97988.48 Da        Isoelectric Point: 10.6063

>WP_169073250.1 TraI/MobA(P) family conjugative relaxase [Escherichia coli]
MISRHIPMRNVKKSSFFRLAEYLQDSQNKQERVGAVKTTNCHQENILDAARYEIEPTQRLNTRAKGDKTY
HLVISFRPGENPPQEVLDVIEERMCTALGYGEHQRVSAVHYDTDNVHIQIAINKVHPKTHNIHTPYNDYK
IRTAVCQELEKEFGLEKDNHRASKTRSENNAADMERHSGRESLLGWIKRECADQLKSAANWQELHKAMRQ
NGLELRQKGNGFVITGPDGLAVKASSVDRSLSRAALEKKLGSFESSIPDTKPSRLSPEAIKQRFGSAVHV
KRPGERPPPIRENRQVHLGEIPVMSINNGKRYEGKPLGSQGASTSVLYGRYQSEQQRLTAARNEALRLAK
VKRDRLIENAKRTGRLKRAAIKLLKGPGVNKRHLYGLASKALGEELERANAIYKKDRQAAYDIHKRRTWA
DWLQFKAAGGDDEALKILRQRNAQQARKEANILHGDKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIRDG
GKRLDVSRDAGDDGLHAALLMAKHRYGDKLTVNGSFEFKKRIVAVAAARELDVSFTDPELEKLRQILAKK
AAREREYIHIPFEQRGKARQAGAMWDTEKKSWFVGPYATRDRIENFIRMNEEKDNEQRRQRYAGTERTEA
GRERSGNGNATGANEPGVTGRSPSADAGNGYGTSNSGRRTGSGDGVTTAGSQPGRSDDTRTGRSRAAESN
GERGHRKPNVARPGRQPPPTAKNRLRAMSELGMVRIAGGSEVLLPGNVPDQLEHKKAERTDQLRRDMAGA
GGITTPAEVKKPLSSAEKYINEREQKRLKGFDIPKHRGYNSTDKGLFEYAGQRKSGLETLALLKRDDEIV
VMPVSDYTARRLSNLKVGESVTLTPSGSVRTTKGRSR

  Protein domains


Predicted by InterproScan.

Domain of unknown function (DUF5710) (DUF5710)

(565-606)

Large polyvalent protein-associated domain 7 (LPD7)

(471-555)

Relaxase/Mobilisation nuclease domain (Relaxase)

(12-251)


  Protein structure



No available structure.




T4CP

ID   994 GenBank   WP_001231127
Name   t4cp2_C9173_RS23280_FWSEC0398|unnamed3 insolico UniProt ID   _
Length   647 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 647 a.a.        Molecular weight: 71661.84 Da        Isoelectric Point: 8.3842

>WP_001231127.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacteriaceae]
MRKPNNAVGPQVRNKKKAAKGKLIPAMAITSLAAGIQAATQYFAYSFNYQELLGPNFNHIYAPWSYFKWY
SAWHERLPDAFFAAGSVGAAVAAGGLVLTALTSMMKANSSKANEYLHGSARWADEQDIRDAGLLNDEGVY
VGAWEDKNGQLRYLRHNGPEHVLTYAPTRSGKGVGLVVPTLLSWKHSTVVADLKGELWAMTAGWRKEYAK
NLCLKFEPAAANGSVAWNPLDEIRVGTENEVGDVQNLATLIVDPDGKGLNDHWQKTSQALLVGVILHVLY
KHKNEGTPATLPYVDSIMADPERGAGELWMEMTQYSHINGENHPVVGAAGRDMMDRPEEEAGSVLSTAKS
YLALFRDPVVARNVSESHFKIKDLMNHDDPVSLYIVTQPNDKARLRPLVRIMLNMIVRLLADKMEFERVD
NNLTLWQRFKQAFGFSVQGTKRVQTKKTYKHRLLGMIDEFPSLGKLEIIQESLAFLAGYGIKFYLICQDI
NQLRSRETGYGPDETITSNCHVQNAYPPNRTETAEHLSKLTGQTTVIKEQITTSGKRASAILGGVSKTIQ
EVQRPLLTVDECLRMPGPKKNAEGLITERGDMVIYVAGFPAIYGKQPLFFQDEIFSLRASVPAPKVTDRI
RAVAAANDDASNEAIAI

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(445-629)

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(121-417)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 1109..11403

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9173_RS23115 (C9173_23185) 1..774 - 774 WP_001146459 transglycosylase SLT domain-containing protein -
C9173_RS23120 (C9173_23190) 790..1098 - 309 WP_023146507 hypothetical protein -
C9173_RS23125 (C9173_23195) 1109..2653 - 1545 WP_024187738 P-type conjugative transfer protein TrbL virB6
C9173_RS23130 (C9173_23200) 2658..2831 - 174 WP_019842051 entry exclusion lipoprotein TrbK -
C9173_RS23135 (C9173_23205) 2844..3614 - 771 WP_023146486 P-type conjugative transfer protein TrbJ virB5
C9173_RS23140 (C9173_23210) 3627..5021 - 1395 WP_031626058 TrbI/VirB10 family protein virB10
C9173_RS23145 (C9173_23215) 5025..5468 - 444 WP_000642284 hypothetical protein -
C9173_RS23150 (C9173_23220) 5471..6361 - 891 WP_000748212 P-type conjugative transfer protein TrbG virB9
C9173_RS23155 (C9173_23225) 6377..7084 - 708 WP_000765386 VirB8/TrbF family protein virB8
C9173_RS23160 (C9173_23230) 7081..9624 - 2544 WP_001090276 VirB4 family type IV secretion/conjugal transfer ATPase virb4
C9173_RS23165 (C9173_23235) 9621..9947 - 327 WP_001208102 conjugal transfer protein TrbD virB3
C9173_RS23170 (C9173_23240) 9950..10333 - 384 WP_023352257 TrbC/VirB2 family protein virB2
C9173_RS23175 (C9173_23245) 10447..11403 - 957 WP_021519988 P-type conjugative transfer ATPase TrbB virB11
C9173_RS23180 (C9173_23250) 11586..11990 - 405 WP_021294253 transcriptional regulator -
C9173_RS23185 (C9173_23255) 12095..12469 + 375 WP_023352258 single-stranded DNA-binding protein -
C9173_RS23190 (C9173_23260) 12544..13422 + 879 WP_019842081 plasmid replication initiator TrfA -
C9173_RS23195 (C9173_23265) 14574..15041 + 468 WP_023352239 antirestriction protein -
C9173_RS23200 (C9173_23270) 15089..16207 + 1119 WP_023352240 hypothetical protein -

Host bacterium

ID   1986 GenBank   NZ_RRNV01000097
Plasmid name   FWSEC0398|unnamed3 Incompatibility group   IncP1
Plasmid size   41775 bp Coordinate of oriT [Strand]   23801..23903 [-]
Host baterium   Escherichia coli strain FWSEC0398

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -