Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 101540 |
| Name | oriT_p7861H_3  |
| Organism | Klebsiella pneumoniae strain 7861H |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_JAJAGC010000011 (19365..19414 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_p7861H_3
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 1366 | GenBank | WP_004153029 |
| Name | traI_LGA70_RS27055_p7861H_3 |
UniProt ID | A0A422WEA5 |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190808.90 Da Isoelectric Point: 6.2081
>WP_004153029.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGQQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKKAEQTAREMAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGQQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKKAEQTAREMAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 993 | GenBank | WP_004152630 |
| Name | traD_LGA70_RS27050_p7861H_3 |
UniProt ID | _ |
| Length | 769 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 769 a.a. Molecular weight: 85951.02 Da Isoelectric Point: 5.0632
>WP_004152630.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKITEGFIPRRLDTRVDARLSALLEEREAEGSLARALFTPDAPASGPADTDSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAAEPSVRATEPPVLRVTTVPLIKPKAAAAAAAASTASSAGIPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTGFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKITEGFIPRRLDTRVDARLSALLEEREAEGSLARALFTPDAPASGPADTDSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAAEPSVRATEPPVLRVTTVPLIKPKAAAAAAAASTASSAGIPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 18807..47225
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LGA70_RS26835 (LGA70_26830) | 13824..14174 | + | 351 | WP_004152758 | hypothetical protein | - |
| LGA70_RS26840 (LGA70_26835) | 14810..15166 | + | 357 | WP_004152717 | hypothetical protein | - |
| LGA70_RS26845 (LGA70_26840) | 15227..15439 | + | 213 | WP_004152718 | hypothetical protein | - |
| LGA70_RS26850 (LGA70_26845) | 15450..15674 | + | 225 | WP_004152719 | hypothetical protein | - |
| LGA70_RS26855 (LGA70_26850) | 15755..16075 | + | 321 | WP_004152720 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| LGA70_RS26860 (LGA70_26855) | 16065..16343 | + | 279 | WP_004152721 | helix-turn-helix transcriptional regulator | - |
| LGA70_RS26865 (LGA70_26860) | 16344..16757 | + | 414 | WP_004152722 | helix-turn-helix domain-containing protein | - |
| LGA70_RS26870 (LGA70_26865) | 17591..18412 | + | 822 | WP_004178064 | DUF932 domain-containing protein | - |
| LGA70_RS26875 (LGA70_26870) | 18445..18774 | + | 330 | WP_011977736 | DUF5983 family protein | - |
| LGA70_RS26880 (LGA70_26875) | 18807..19292 | - | 486 | WP_004178063 | transglycosylase SLT domain-containing protein | virB1 |
| LGA70_RS26885 (LGA70_26880) | 19726..20118 | + | 393 | WP_004178062 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| LGA70_RS26890 (LGA70_26885) | 20332..21027 | + | 696 | WP_004178061 | transcriptional regulator TraJ family protein | - |
| LGA70_RS26895 (LGA70_26890) | 21111..21482 | + | 372 | WP_004208838 | TraY domain-containing protein | - |
| LGA70_RS26900 (LGA70_26895) | 21536..21904 | + | 369 | WP_004178060 | type IV conjugative transfer system pilin TraA | - |
| LGA70_RS26905 (LGA70_26900) | 21918..22223 | + | 306 | WP_004178059 | type IV conjugative transfer system protein TraL | traL |
| LGA70_RS26910 (LGA70_26905) | 22243..22809 | + | 567 | WP_004152602 | type IV conjugative transfer system protein TraE | traE |
| LGA70_RS26915 (LGA70_26910) | 22796..23536 | + | 741 | WP_004152601 | type-F conjugative transfer system secretin TraK | traK |
| LGA70_RS26920 (LGA70_26915) | 23536..24960 | + | 1425 | WP_004152600 | F-type conjugal transfer pilus assembly protein TraB | traB |
| LGA70_RS26925 (LGA70_26920) | 24953..25549 | + | 597 | WP_004152599 | conjugal transfer pilus-stabilizing protein TraP | - |
| LGA70_RS26930 (LGA70_26925) | 25572..26141 | + | 570 | WP_004152598 | type IV conjugative transfer system lipoprotein TraV | traV |
| LGA70_RS26935 (LGA70_26930) | 26273..26683 | + | 411 | WP_004152597 | lipase chaperone | - |
| LGA70_RS26940 (LGA70_26935) | 26688..26978 | + | 291 | WP_004152596 | hypothetical protein | - |
| LGA70_RS26945 (LGA70_26940) | 27002..27220 | + | 219 | WP_004171484 | hypothetical protein | - |
| LGA70_RS26950 (LGA70_26945) | 27221..27538 | + | 318 | WP_004152595 | hypothetical protein | - |
| LGA70_RS26955 (LGA70_26950) | 27605..28009 | + | 405 | WP_004152594 | hypothetical protein | - |
| LGA70_RS26960 (LGA70_26955) | 28305..28703 | + | 399 | WP_004153071 | hypothetical protein | - |
| LGA70_RS26965 (LGA70_26960) | 28775..31414 | + | 2640 | WP_004152592 | type IV secretion system protein TraC | virb4 |
| LGA70_RS26970 (LGA70_26965) | 31414..31803 | + | 390 | WP_004152591 | type-F conjugative transfer system protein TrbI | - |
| LGA70_RS26975 (LGA70_26970) | 31803..32429 | + | 627 | WP_004152590 | type-F conjugative transfer system protein TraW | traW |
| LGA70_RS26980 (LGA70_26975) | 32473..33432 | + | 960 | WP_015065634 | conjugal transfer pilus assembly protein TraU | traU |
| LGA70_RS26985 (LGA70_26980) | 33445..34083 | + | 639 | WP_004152682 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| LGA70_RS26990 (LGA70_26985) | 34131..36086 | + | 1956 | WP_004153093 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| LGA70_RS26995 (LGA70_26990) | 36118..36354 | + | 237 | WP_004152683 | conjugal transfer protein TrbE | - |
| LGA70_RS27000 | 36351..36536 | + | 186 | WP_004152684 | hypothetical protein | - |
| LGA70_RS27005 (LGA70_26995) | 36582..36908 | + | 327 | WP_004152685 | hypothetical protein | - |
| LGA70_RS27010 (LGA70_27000) | 36929..37681 | + | 753 | WP_004152686 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| LGA70_RS27015 (LGA70_27005) | 37692..37931 | + | 240 | WP_004152687 | type-F conjugative transfer system pilin chaperone TraQ | - |
| LGA70_RS27020 (LGA70_27010) | 37903..38475 | + | 573 | WP_004152688 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| LGA70_RS27025 (LGA70_27015) | 38468..38896 | + | 429 | WP_004152689 | hypothetical protein | - |
| LGA70_RS27030 (LGA70_27020) | 38883..40253 | + | 1371 | WP_004178055 | conjugal transfer pilus assembly protein TraH | traH |
| LGA70_RS27035 (LGA70_27025) | 40253..43102 | + | 2850 | WP_004178054 | conjugal transfer mating-pair stabilization protein TraG | traG |
| LGA70_RS27040 (LGA70_27030) | 43108..43635 | + | 528 | WP_004153030 | hypothetical protein | - |
| LGA70_RS27045 (LGA70_27035) | 43825..44556 | + | 732 | WP_004152629 | conjugal transfer complement resistance protein TraT | - |
| LGA70_RS27050 (LGA70_27040) | 44916..47225 | + | 2310 | WP_004152630 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
| ID | 1984 | GenBank | NZ_JAJAGC010000011 |
| Plasmid name | p7861H_3 | Incompatibility group | IncFII |
| Plasmid size | 68762 bp | Coordinate of oriT [Strand] | 19365..19414 [-] |
| Host baterium | Klebsiella pneumoniae strain 7861H |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |