Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101539
Name   oriT_p7861H_1 in_silico
Organism   Klebsiella pneumoniae strain 7861H
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JAJAGC010000010 (98438..98588 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_p7861H_1
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1365 GenBank   WP_001337757
Name   mobH_LGA70_RS26315_p7861H_1 insolico UniProt ID   A0A709FRI2
Length   992 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A709FRI2


T4CP

ID   991 GenBank   WP_000178856
Name   traD_LGA70_RS26320_p7861H_1 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   992 GenBank   WP_000637386
Name   traC_LGA70_RS26400_p7861H_1 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 96522..121713

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGA70_RS26305 (LGA70_26290) 92547..92780 - 234 WP_001191892 hypothetical protein -
LGA70_RS26310 (LGA70_26295) 92762..93379 - 618 WP_001249396 hypothetical protein -
LGA70_RS26315 (LGA70_26300) 93547..96525 + 2979 WP_001337757 MobH family relaxase -
LGA70_RS26320 (LGA70_26305) 96522..98387 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
LGA70_RS26325 (LGA70_26310) 98437..98982 + 546 WP_000228720 hypothetical protein -
LGA70_RS26330 (LGA70_26315) 98939..99568 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
LGA70_RS26335 (LGA70_26320) 99578..100024 + 447 WP_000122506 hypothetical protein -
LGA70_RS26340 (LGA70_26325) 100034..100411 + 378 WP_000869261 hypothetical protein -
LGA70_RS26345 (LGA70_26330) 100411..101073 + 663 WP_001231463 hypothetical protein -
LGA70_RS26350 (LGA70_26335) 101254..101397 + 144 WP_001275801 hypothetical protein -
LGA70_RS26355 (LGA70_26340) 101409..101774 + 366 WP_001052531 hypothetical protein -
LGA70_RS26360 (LGA70_26345) 101919..102200 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
LGA70_RS26365 (LGA70_26350) 102197..102823 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
LGA70_RS26370 (LGA70_26355) 102807..103724 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
LGA70_RS26375 (LGA70_26360) 103724..105037 + 1314 WP_024131605 TraB/VirB10 family protein traB
LGA70_RS26380 (LGA70_26365) 105034..105612 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
LGA70_RS26385 (LGA70_26370) 105616..106008 + 393 WP_000479535 TraA family conjugative transfer protein -
LGA70_RS26390 (LGA70_26375) 106209..111740 + 5532 WP_109829093 Ig-like domain-containing protein -
LGA70_RS26395 (LGA70_26380) 111889..112596 + 708 WP_001259347 DsbC family protein -
LGA70_RS26400 (LGA70_26385) 112593..115040 + 2448 WP_000637386 type IV secretion system protein TraC virb4
LGA70_RS26405 (LGA70_26390) 115055..115372 + 318 WP_000351984 hypothetical protein -
LGA70_RS26410 (LGA70_26395) 115369..115899 + 531 WP_001010738 S26 family signal peptidase -
LGA70_RS26415 (LGA70_26400) 115862..117127 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
LGA70_RS26420 (LGA70_26405) 117124..117795 + 672 WP_001337754 EAL domain-containing protein -
LGA70_RS26425 (LGA70_26410) 117795..118799 + 1005 WP_043940352 TraU family protein traU
LGA70_RS26430 (LGA70_26415) 118915..121713 + 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
LGA70_RS26435 (LGA70_26420) 121752..122612 - 861 WP_000709517 hypothetical protein -
LGA70_RS26440 (LGA70_26425) 122735..123376 - 642 WP_000796664 hypothetical protein -
LGA70_RS26445 (LGA70_26430) 123670..123990 + 321 WP_000547566 hypothetical protein -
LGA70_RS26450 (LGA70_26435) 124294..124479 + 186 WP_001186917 hypothetical protein -
LGA70_RS26455 (LGA70_26440) 124699..125667 + 969 WP_000085162 AAA family ATPase -
LGA70_RS26460 (LGA70_26445) 125678..126586 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   1983 GenBank   NZ_JAJAGC010000010
Plasmid name   p7861H_1 Incompatibility group   IncA/C2
Plasmid size   172930 bp Coordinate of oriT [Strand]   98438..98588 [+]
Host baterium   Klebsiella pneumoniae strain 7861H

Cargo genes

Drug resistance gene   erm(42), rmtB, tet(G), floR, aadA2, dfrA12, aac(3)-IId, aph(6)-Id, aph(3'')-Ib, sul2, blaCTX-M-14
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -