Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   101534
Name   oriT_pAA578 in_silico
Organism   Escherichia coli strain DDWI-01
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JAZGLZ010000080 (1033..1189 [+], 157 nt)
oriT length   157 nt
IRs (inverted repeats)      101..108, 118..125  (ACAAACTC..GAGTTTGT)
Location of nic site      65..66
Conserved sequence flanking the
  nic site    		  ACTGGCATAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 157 nt

>oriT_pAA578
GTAACTCCGTGCGCGGTGAAAAATCGCATTTTAGCGCGTCACTGGTAGTTTAAAAACTGAACTGGCATAATGCACAGCAAATCACGAAGTGCGCACTTATACAAACTCTCCTTCGTCGAGTTTGTGTGCGCCCTGCGGGGCTAAAAGAAAACGGCAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   1362 GenBank   WP_011666798
Name   mobQ_V2H89_RS23645_pAA578 insolico UniProt ID   A0A1K8
Length   715 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 715 a.a.        Molecular weight: 81916.31 Da        Isoelectric Point: 7.1893

>WP_011666798.1 MULTISPECIES: MobQ family relaxase [Enterobacteriaceae]
MAIYHLSMKILSRSKGYSAVASAAYRAGEKILDERTGVIHDYTRKNGVASAVILTPANAPAWCANRAELW
NAVEKAERRKNSQLAREFELAIPRELAQDAARETVLNFVRENFVSRGMIADVAFHNLGGSNPHAHIMLSM
RAITPDGFGEKVREWNDWTHAETWRGSWADHANRALANAGYQEEIDHRSYERQGVEKTPGIHLGKSACAM
EKWGIETERGEQNRLINRLNLEIQVSRDELRNSELTEPARRMADLLNIELPDNATSYILHDIIEALPTDS
NAAWKLTSKAMSMMADMQATRRRWEDLNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWVAPEYGREQEK
IQSLSQRMEKLRQHHWKVEKQDIPASQKAFETQWQQWGASGLADLREQLRKREEELRRKEQEETERRRAE
QLRRNDNAVMDSGVLKAVVTGYGEAPMPGNGEMTCYLLLHNRNGEYTLWGNELEKYRARIFESVDLMRDR
SGYICERSEIGQRPPLQHVYSSATFEQLLTQVCQTWPQHTRDLRQPKTWPESFCLGEDRQPAMPSLAARK
VDFTQGRLPPTLMPVMSSVDRETRQLQLLLVMGVDDSMGGVVRLNGTLYPAFAVPTADNSQLVINALTDK
GLRFAGYGEAVNHDADSPARPAPELMQFHLKTWSEPLFAIVNTPEKQPDHLFRSLGFERTWDEWKRDEYA
RTHATERRHDRGWSQ

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(18-212)


  Protein structure


Source ID Structure
AlphaFold DB A0A1K8


Host bacterium

ID   1978 GenBank   NZ_JAZGLZ010000080
Plasmid name   pAA578 Incompatibility group   -
Plasmid size   4091 bp Coordinate of oriT [Strand]   1033..1189 [+]
Host baterium   Escherichia coli strain DDWI-01

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -